Effects of microenvironmental factors on assessing nanoparticle toxicity

Seo, Suyoung; Lee, Ji Eun; Lee, Kangwon; Kim, Hong Nam

doi:10.1039/d1en00925g

Cited by 7 publications

(6 citation statements)

References 195 publications

(239 reference statements)

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“…However, these models have several limitations in accurately assessing PM-induced tissue toxicity and its underlying mechanisms. [14] In the 2D culture system, the culture environment loses the 3D extracellular matrix-based architecture and cellular interactions owing to the dissolution of cell-secreted cytokines and limited cell numbers in co-culture. [15,16] The absence of these factors implies a low relevance in vivo, and these dissimilarities make it difficult to accurately predict PM-induced cytotoxic effects.…”

Section: Introductionmentioning

confidence: 99%

Neuro‐Glia‐Vascular‐on‐a‐Chip System to Assess Aggravated Neurodegeneration via Brain Endothelial Cells upon Exposure to Diesel Exhaust Particles

Seo

Jang

Kim

et al. 2022

Adv Funct Materials

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Air pollution induces neurodegeneration, including cognitive deficits, neuroinflammation, and disruption of the blood-brain barrier. The mechanisms underlying air pollution-mediated neurodegeneration have not yet been fully elucidated given the limited knowledge on intercellular interactions. A brainon-a-chip platform is presented comprising neurons, glia, and brain endothelial cells (bECs; neuro-glia-vascular, NGV) and diesel exhaust particle (DEP)-induced neurodegeneration is evaluated with a particular focus on the intercellular interactions. DEP exposure in the NGV model yields Alzheimer's diseaselike signatures, including amyloid beta accumulation, tau phosphorylation, hydrogen peroxide (H 2 O 2 )/reactive oxygen species (ROS) production, and neuronal cell death. bEC-secreted granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates microglial activation and the overproduction of H 2 O 2 / ROS in microglia, suggesting that the bEC-microglia-neuron is a neurodegeneration cascade. Pharmacological inhibition at each step of the cascade, including GM-CSF neutralization, microglial activation suppression, and ROS scavenging, prohibits neurodegeneration in the NGV model. Therefore, intercellular interactions should be further studied of air pollution-induced neurodegeneration.

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Section: Introductionmentioning

confidence: 99%

Neuro‐Glia‐Vascular‐on‐a‐Chip System to Assess Aggravated Neurodegeneration via Brain Endothelial Cells upon Exposure to Diesel Exhaust Particles

Seo

Jang

Kim

et al. 2022

Adv Funct Materials

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“…The radius of curvature of the nanoparticles is a factor for protein corona formation. A planar surface is provided by a large radius of curvature, thus resulting in more effective protein binding [29].…”

Section: Morphologymentioning

confidence: 99%

Toxicity Evaluation and Biocompatibility of Nanostructured Biomaterials

Thomas¹,

Varghese²,

Kalarikkal³

et al. 2023

Cytotoxicity - Understanding Cellular Damage and Response

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Biomaterials have occupied a prominent place in regenerative procedures to restore human health. Moreover, there is a greater need in understanding, analyzing and establishing their toxicity profile. These, when made into nano-sized constructions called nanostructured biomaterials, their regenerative potential is enhanced, which could influence their toxicity nature. This chapter intends to give comprehensive information on their nanotoxicology pathways at the cellular level, their entry pathways into the human body, and their potential consequences on human health. It clearly explains the cytocompatibility and biocompatibility of various nanostructured biomaterials for potential human health applications like drug delivery and tissue engineering. A detailed overview of various in vitro and in vivo evaluation methods of biocompatibility of nanomaterials are outlined in this chapter that researchers should address as they move forward in developing new systems for the field of regeneration.

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“…Another critical problem in the current studies on MSN toxicity is the poor correlation between in vitro and in vivo results caused by the complex physiological environment. In traditional in vitro cell models, cells are usually cultured under the static condition and the introduced nanoparticles tend to deposit on the cells, which is very different from the fluidic environment in the body . Although in vivo animal models mimic the circulation of nanoparticles in the blood, it is difficult to monitor the interaction between nanoparticles and the body in real time and explain the underlying mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…In traditional in vitro cell models, cells are usually cultured under the static condition and the introduced nanoparticles tend to deposit on the cells, which is very different from the fluidic environment in the body. 22 Although in vivo animal models mimic the circulation of nanoparticles in the blood, it is difficult to monitor the interaction between nanoparticles and the body in real time and explain the underlying mechanism. To address these challenges, an in vitro blood flow model has been developed to simulate the transportation of nanoparticles in the bloodstream, which has been utilized to accurately predict the biological behavior, 23−27 targetability, 28−31 and toxicity 32−34 of various nanoparticles.…”

Section: ■ Introductionmentioning

confidence: 99%

Shape and Shear Stress Impact on the Toxicity of Mesoporous Silica Nanoparticles: In Vitro and In Vivo Evidence

et al. 2023

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Mesoporous silica nanoparticles (MSNs) are widely used in the biomedical field because of their unique and excellent properties. However, the potential toxicity of different shaped MSNs via injection has not been fully studied. This study aims to systematically explore the impact of shape and shear stress on the toxicity of MSNs after injection. An in vitro blood flow model was developed to investigate the cytotoxicity and the underlying mechanisms of spherical MSNs (S-MSN) and rodlike MSNs (R-MSN) in human umbilical vein endothelial cells (HUVECs). The results suggested that the interactions between MSNs and HUVECs under the physiological flow conditions were significantly different from that under static conditions. Whether under static or flow conditions, R-MSN showed better cellular uptake and less oxidative damage than S-MSN. The main mechanism of cytotoxicity induced by R-MSN was due to shear stress-dependent mechanical damage of the cell membrane, while the toxicity of S-MSN was attributed to mechanical damage and oxidative damage. The addition of fetal bovine serum (FBS) alleviated the toxicity of S-MSN by reducing cellular uptake and oxidative stress under static and flow conditions. Moreover, the in vivo results showed that both S-MSN and R-MSN caused cardiovascular toxicity in zebrafish and mouse models due to the high shear stress, especially in the heart. S-MSN led to severe oxidative damage at the accumulation site, such as liver, spleen, and lung in mice, while R-MSN did not cause significant oxidative stress. The results of in vitro blood flow and in vivo models indicated that particle shape and shear stress are crucial to the biosafety of MSNs, providing new evidence for the toxicity mechanisms of the injected MSNs.

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Effects of microenvironmental factors on assessing nanoparticle toxicity

Abstract: Nanoparticles, such as dust or fine particles produced from diverse sources, are regarded as hazardous materials to human organs, and the interest in understanding their biological mechanisms and evaluating the...

Cited by 7 publications

References 195 publications

Neuro‐Glia‐Vascular‐on‐a‐Chip System to Assess Aggravated Neurodegeneration via Brain Endothelial Cells upon Exposure to Diesel Exhaust Particles

Neuro‐Glia‐Vascular‐on‐a‐Chip System to Assess Aggravated Neurodegeneration via Brain Endothelial Cells upon Exposure to Diesel Exhaust Particles

Toxicity Evaluation and Biocompatibility of Nanostructured Biomaterials

Shape and Shear Stress Impact on the Toxicity of Mesoporous Silica Nanoparticles: In Vitro and In Vivo Evidence

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