Protein composition of follicular fluid and oocyte cleavage occurrence in in vitro fertilization (IVF)

Gonzalès, Jacques; Lesourd, Sylvie; Dreden, Patrick Van; Richard, Peter; Lefèbvre, Gilles; Brouzes, D. Vauthier

doi:10.1007/bf01203815

Cited by 17 publications

(10 citation statements)

References 21 publications

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“…Gonzales et al (1992) found significantly higher concentrations of the C3 complement fraction in FF from oocytes that had been fertilized in comparison with those non-fertilized, which confirms our study [47]. …”

Section: Discussionsupporting

confidence: 92%

A Proteomic Analysis of Human Follicular Fluid: Comparison between Younger and Older Women with Normal FSH Levels

Hashemitabar

Bahmanzadeh

Mostafaie

et al. 2014

IJMS

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The follicular fluid (FF) is produced during folliculogenesis and contains a variety of proteins that play important roles in follicle development and oocyte maturation. Age-related infertility is usually considered as a problem that can be solved by assisted reproduction technology. Therefore, the identiﬁcation of novel biomarkers that are linked to reproductive aging is the subject of this study. FF was obtained from healthy younger (20–32 years old) and older (38–42 years old) women undergoing intracytoplasmic sperm injection (ICSI) due to male factor infertility. The FF was analyzed by two-dimensional gel electrophoresis (2-DE). The power of two-dimensional gel electrophoresis and the identiﬁcation of proteins were exploited using matrix-assisted laser desorption-ionization time-of-flight/time-of-flight (MALDI-TOF-TOF) mass spectrometry. Twenty three protein spots showed reproducible and significant changes in the aged compared to the young group. Of these, 19 protein spots could be identified using MALDI-TOF-TOF-MS. As a result of MASCOT search, five unique downregulated proteins were identified in the older group. These were identified as serotransferrin, hemopexin precursor, complement C3, C4 and kininogen. A number of protein markers were found that may help develop diagnostic methods of infertility.

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Section: Discussionsupporting

confidence: 92%

A Proteomic Analysis of Human Follicular Fluid: Comparison between Younger and Older Women with Normal FSH Levels

Hashemitabar

Bahmanzadeh

Mostafaie

et al. 2014

IJMS

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“…Some of the proteins present in follicular fluid are derived from plasma, whereas others are locally synthesized within the follicle, which accounts for the similar, but not identical, protein profiles observed in these compartments [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Thrombin Generation and Presence of Thrombin Receptor in Ovarian Follicles1

Roach

Petrik

Plante³

et al. 2002

Biology of Reproduction

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Prothrombin, once converted to its enzymatically active form (i.e., thrombin), induces a broad spectrum of cellular responses in both vascular and avascular tissues. Bovine ovarian granulosa cells isolated from healthy follicles of various sizes contain both prothrombin mRNA and immunologically reactive prothrombin that appears to be identical to prothrombin in follicular fluid and plasma. When tissue factor, the primary physiological activator of thrombin generation in plasma, is used to initiate thrombin formation, the profile of prothrombin-to-thrombin conversion is similar in follicular fluid and plasma. The conclusion that biologically functional prothrombin is synthesized by granulosa cells is further supported by evidence that mRNA for gamma-glutamyl carboxylase, an enzyme essential for the vitamin K-dependent posttranslational modification of prothrombin, is expressed in granulosa cells in a manner similar to prothrombin mRNA. Thrombin's biological effects are mediated through selective proteolytic cleavage and activation of specific receptors. Bovine granulosa cells possess thrombin receptor (PAR-1) mRNA, and as seen with prothrombin mRNA and gamma-glutamyl carboxylase mRNA, cells isolated from small follicles possess more PAR-1 mRNA than cells from large follicles. Thrombin receptor expression by cells in close proximity to an active thrombin-generating system suggests that these factors may be important mediators of cellular function in the ovarian follicle.

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“…Moreover, granulosa cells could play an active role in altering the FF by selectively filtering and perhaps transforming some of the metabolites. There is increasing interest in determining whether the metabolic composition of the FF can provide information about the quality of the oocytes and embryo viability (13)(14)(15)(16)(17), or even about the development of possible diseases in the adult (18). The studies that (www.interscience.wiley.com) DOI:10.1002/nbm.…”

Section: Introductionmentioning

confidence: 99%

NMR metabolic profile of human follicular fluid

et al. 2010

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The environment of the oocyte during its in vivo maturation consists of follicular fluid (FF) and is surrounded by granulosa cells. The FF is derived from the sanguineous plasma and secretions, synthesised in the follicle wall, that contain a large variety of growth factors, cytokines, amino acids, and other metabolites. These metabolites are presumably involved in the physiology of the oocyte. The identification, quantification and study of FF metabolites can provide additional information about the oocyte state which can be helpful in distinguishing those oocytes that have a greater capacity to be fertilised and to develop properly. The aim of this work is to identify the metabolic profile of FF samples exhaustively using High Resolution Nuclear Magnetic Resonance (NMR). A total of 30 FF samples from oocyte donors (<35 years) were analysed. Different monodimensional (1D) and bidimensional (2D) (homo and heteronuclear) NMR experiments were acquired. A total of 131 chemical shifts were assigned and 42 metabolites, including as example glucose, lactate, acetate, acetoacetate, pyruvate and b-hydroxybutyrate, were identified. High correlations were found between these important intermediaries of the energetic metabolic pathways of the follicle which can indicate the importance of these pathways in oocyte development. Some of these identified metabolites might be useful as biomarkers of the follicular maturation state, allowing oocytes with a higher fertilisation potential to be selected, thereby increasing pregnancy rates in women following in vitro fertilisation (IVF) treatments.

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Protein composition of follicular fluid and oocyte cleavage occurrence in in vitro fertilization (IVF)

Cited by 17 publications

References 21 publications

A Proteomic Analysis of Human Follicular Fluid: Comparison between Younger and Older Women with Normal FSH Levels

A Proteomic Analysis of Human Follicular Fluid: Comparison between Younger and Older Women with Normal FSH Levels

Thrombin Generation and Presence of Thrombin Receptor in Ovarian Follicles1

NMR metabolic profile of human follicular fluid

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