Protein composition of crevicular fluid before and after treatment

Abstract

Grevicular fluid (GF) analysis is a potential tool for site-speciflc diagnosis of periodontal disease activity. In this study, GE was collected using a novel washing method from 91 sites in 18 adult periodontitis patients both before and after conventional periodontal treatment. The sites studied were classified according to their clinical status and the number of polymorphonuclear leukocytes (PMN's) in GE samples. GF proteins were analyzed from individual sites with gel electrophoresis (SDS-PAGE). Furthermore, both the cell-bound and soluble neutral proteolytie activities of the samples were determined. Albumin was the main protein both in healthy and slightly inflamed sites. The most severely inflamed sites were characterized by high levels of low molecular weight (LMW) proteins (mol. weight ca. 12 000) and strong cell-bound neutral proteolytie activity. Scaling and root planing reduced both the LMW proteins and neutral proteolytie activity markedly in pockets responding well to treatment. The levels ofthe LMW proteins in GF correlated with the cell-bound neutral proteolytie activity, which reflected the number of PMN's in the sample. The present results suggest that the appearance of the LMW proteins in GF is associated with the periodontal inflammatory status of the site.

Effects of various culture environments on expression of major outer membrane proteins fromPorphyromonas gingivalis

“…Although the outer fluid after dialysis would be rich in lower molecular mass components, outer membrane protein levels were not affected (data not shown). It has been suggested that the appearance of low molecular mass proteins in crevicular fluid is associated with the periodontal inflammatory status [33]. Moreover, asaccharolytic P. gingivalis has been known to utilize mainly peptides instead of single amino acids [21,34,35].…”

Inhibition of Sodium Hypochlorite Antimicrobial Activity in the Presence of Bovine Serum Albumin

“…It is present in inflammatory exudates (19,20), gingival crevicular fluid (24,25), and dentinal fluid (26). Its concentration in plasma is between 3.5% and 5% and up to 4% in inflammatory exudate (36).…”

“…Albumin is the main protein in human serum, and it is also a major component of inflammatory exudate (22,23). Its presence in gingival crevicular fluid and in the fluid from dentinal tubules has also been reported (24)(25)(26). In this study, the effect of bovine serum albumin on the antibacterial activity of NaOCl against four different microorganisms was investigated.…”

An in vitro study of plasticized poly(lactic‐co‐glycolic acid) films as possible guided tissue regeneration membranes: Material properties and drug release kinetics

“…Such additional in vitro investigations might include: (1) Monitoring degradation in a crevicular fluid like solution. Crevicular fluid contains high amounts of albumin 65, 66. A study by Çatiker et al67 showed that the hydrolytic breakdown of PLGA is strongly influenced by the presence of BSA as it increases the initial velocity of the hydrolytic de‐esterification process of glycolic‐acid containing copolymer.…”