Inhibition of Sodium Hypochlorite Antimicrobial Activity in the Presence of Bovine Serum Albumin

Pappen, Fernanda Geraldo; Qian, Wei; Aleksejūnienė, Jolanta; Leonardo, Renato de Toledo; Leonardo, Mário Roberto; Haapasalo, Markus

doi:10.1016/j.joen.2009.09.025

Cited by 35 publications

(19 citation statements)

References 35 publications

(54 reference statements)

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“…Thus, the aim of the treatment is clear: ''No bacteria-No reaction.'' One problem of root canal disinfection in vivo is the ability of organic compounds to inactivate the irrigant antimicrobial activity (23). Because the dentin was infected intraorally, a high quantity of organic structures was expected.…”

Section: Discussionmentioning

confidence: 99%

Biofilm Dissolution and Cleaning Ability of Different Irrigant Solutions on Intraorally Infected Dentin

Carpio-Perochena¹,

Bramante²,

Duarte³

et al. 2011

Journal of Endodontics

101

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Section: Discussionmentioning

confidence: 99%

Biofilm Dissolution and Cleaning Ability of Different Irrigant Solutions on Intraorally Infected Dentin

Carpio-Perochena¹,

Bramante²,

Duarte³

et al. 2011

Journal of Endodontics

101

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“…However, it is highly cytotoxic (7) and its effects are significantly affected by organic matter (8). It was also shown that the clinical performance of NaOCl is inferior to its effects in vitro and about 40-60% of the cases of irrigation with NaOCl still harbor bacteria in the main canal (2).…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm and Antibacterial Activities of Farnesol and Xylitol as Potential Endodontic Irrigants

et al. 2013

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This study investigated the antibiofilm and antibacterial effects of farnesol and xylitol in a series of experiments in order to evaluate their potential use as root canal irrigants. The following substances were tested: 0.2% farnesol; 5% and 20% xylitol; 0.2% farnesol plus 20% xylitol; and saline (control). For comparison with an established endodontic irrigant, 2.5% NaOCl was included in each test. Three experiments were conducted: the crystal violet assay, to evaluate the effects on the biofilm biomass; the dentin disinfection test, to evaluate the effects on bacterial viability in biofilms; and the root canal disinfection test, to simulate the use in the root canal environment. Farnesol was the most effective substance in reducing the biofilm biomass, followed by 20% xylitol. All substances affected bacterial viability in biofilms; farnesol showed the best results followed by the farnesol/ xylitol combination. Irrigation with all substances significantly reduced the bacterial load (p<0.001), but only the farnesol/xylitol combination was significantly more effective than saline (p=0.02). NaOCl was more effective than any other substance tested in the three experiments (p<0.001). The findings demonstrated that farnesol affected both the biofilm biomass and the viability of cells in the biofilm, while 20% xylitol affected only the biofilm biomass. Although not more effective than NaOCl, the combination of these two antibiofilm substances has potential to be used in endodontics in certain situations.

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“…This is because of its slower decomposition, and other factors such as release of hypochlorous acid, stable chlorine levels, low toxicity and low corrosive ability (Mazzola et al 2003). Although it is known that in the presence of organic matter, compounds such as sodium hypochlorite can be less effective in suspension (Pappen et al 2010), the household bleach at the recommended concentration for general use was no less effective in simulated dirty conditions than in clean conditions. The antibacterial effect demonstrated by both disinfectants against the four S. aureus strains in suspension meets the EN 1276 standard which recommends that for a disinfectant product to be antibacterial using the suspension test, 5 log reduction should be achieved within 5 min.…”

Section: Discussionmentioning

confidence: 99%

A comparison of the sensitivity of fourStaphylococcus aureusisolates to two chlorine-based disinfectants and an eco-friendly commercially available cleaning agent

Adukwu¹,

Allen²,

Phillips³

2014

International Journal of Environmental Health Research

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The aim of this study was to determine the effect of household bleach, a sodium dichloroisocyanurate (NaDCC)-based disinfectant and an eco-friendly cleaning agent (EFCA) on four Staphylococcus aureus strains, including two isolated from community infections. The products were assessed using the suspension (EN 1276) and surface (EN 13697) tests, while biofilm activity was determined using the 96-well plate method. Bleach and NaDCC caused > 5 log reduction in viable counts within 5 min in suspension, whilst on surfaces the reduction was < 3 log. Bleach at 5000 ppm free available chlorine completely eradicated MSSA NCTC 13297 and PVL CA MSSA biofilms within 10 min, but not at 500 and 50 ppm, NaDCC was less effective against biofilms. The EFCA demonstrated no antimicrobial activity. It is of concern that at the recommended "use" dilution, bleach did not eradicate biofilms. Although increasing contact time and/or concentration should improve the activity, this may not be acceptable to the user.

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Inhibition of Sodium Hypochlorite Antimicrobial Activity in the Presence of Bovine Serum Albumin

Cited by 35 publications

References 35 publications

Biofilm Dissolution and Cleaning Ability of Different Irrigant Solutions on Intraorally Infected Dentin

Biofilm Dissolution and Cleaning Ability of Different Irrigant Solutions on Intraorally Infected Dentin

Antibiofilm and Antibacterial Activities of Farnesol and Xylitol as Potential Endodontic Irrigants

A comparison of the sensitivity of fourStaphylococcus aureusisolates to two chlorine-based disinfectants and an eco-friendly commercially available cleaning agent

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