Protein binding study of clozapine by capillary electrophoresis in the frontal analysis mode

Characterization of interaction between doxycycline and human serum albumin by capillary electrophoresis‐frontal analysis

“…It is observed that the resolution between the peaks of unbound doxycycline and HSA decreased and the peak became narrower with an increase in applied voltage. The results were in accordance with the previous work for clozapine and protein-binding study by CE-FA [29]. The plateau region in the electropherogram is not flat when 18 kV was used.…”

In Vitro–In Vivo Extrapolation of Clearance: Modeling Hepatic Metabolic Clearance of Highly Bound Drugs and Comparative Assessment with Existing Calculation Methods

“…For the current drugs, the observed value of fu p ranged from 0.0007 to 0.15, whereas human CL ranged from 0.04 to 15 mL/(min kg), covering a large range of properties for highly bound compounds. 2,10,[16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] The dataset is compiled in Table 1. Clearance refers to plasma kinetics, and it was assumed that this is mainly due to hepatic metabolic CL.…”

“…For each drug studied, the main extracellular binding protein considered in this study is AL or AAG [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] (Table 1). However, the works of Burczynski et al, 13 Qin et al, 38 and Bilello et al 39 suggest that proteinfacilitated metabolism, and hence hepatocyte uptake of drugs, is significantly greater with AL as compared with that of AAG at physiologically relevant concentrations (more explanations are provided in the Discussion section for differences between AL and AAG).…”

A competitive low-affinity binding model for determining the mutual and specific sites of two ligands on protein

“…In this article, we propose a chemical model for determining the mutual and specific lowaffinity binding sites. We use HSA as a model protein because it is a major drug carrier protein in blood and has large binding capacity for most drugs and endogenous metabolites [26][27][28][29][30][31]. Two non-steroidal anti-inflammatory drugs, tolmetin (TOL) and salicylic acid (SAL), were used as ligands.…”