Protein-based polyelectrolyte multilayers

Straeten, Aurélien vander; Lefèvre, Damien; Demoustier‐Champagne, Sophie; Dupont‐Gillain, Christine C.

doi:10.1016/j.cis.2020.102161

Cited by 32 publications

(24 citation statements)

References 108 publications

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“…The substrates' specific characteristics in adsorption experiments, primarily their chemical composition, homogeneity, geometrical topology, roughness, charge distribution, stability, etc., are also prerequisites. Recently, various compounds and preparation methods have been explored to improve stability and water-solubility of polymeric drug delivery systems, which enable sustained and efficient delivery of therapeutic protein over prolonged periods, leading to the formulation of DNA [ 37 ], chitosan, polyelectrolytes [ 38 ], pyrrole, silica, gold, cells, and dendrimer-based nanoparticles. Cytotoxicity of the active core of NP and its poor cellular internalization is among the most critical factors that contribute to unfavourable side effects caused by noneffective active compound delivery.…”

Section: Discussionmentioning

confidence: 99%

The role of the electrokinetic charge of neurotrophis-based nanocarriers: protein distribution, toxicity, and oxidative stress in in vitro setting

et al. 2021

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Background The rational chemical design of nanoparticles can be readily controlled and optimized by quantitatively studying protein adsorption at variously charged polymer carriers, determining their fate in biological fluids. We manufactured brain-derived neurotrophic factor (BDNF) -based electrostatic nanocomplexes with a different type of dendrimer core (anionic or cationic), encapsulated or not in polyethylene glycol (PEG), and studied their physicochemical properties and behavior in a biological setting. We investigated whether the electrokinetic charge of dendrimer core influences BDNF loading and desorption from the nanoparticle and serves as a determinant of nanoparticles’ behavior in in vitro setting, influencing mitochondrial dysfunction, lipid peroxidation, and general nanoparticles’ cellular toxicity. Results We found that the electrokinetic charge of the dendrimer core influences nanoparticles in terms of BDNF release profile from their surfaces and their effect on cell viability, mitochondrial membrane potential, cell phenotype, and induction of oxidative stress. The electrostatic interaction of positively charged core of nanoparticles with cell membranes increases their cytotoxicity, as well as serious phenotype alterations compared to negatively charged nanoparticles core in neuron-like differentiated human neuroblastoma cells. Moreover, PEG adsorption at nanoparticles with negatively charged core presents a distinct decrease in metabolic cell activity. On the contrary, charge neutralization due to PEG adsorption on the surface of nanoparticles with positively charged core does not reduce their cytotoxicity, makes them less biocompatible with differentiated cells, and presumably shows non-specific toxicity. Conclusions The surface charge transformation after adsorption of protein or polyelectrolyte during nanocarriers formulation has an important role not only in designing nanomaterials with potent neuroprotective and neuroregenerative properties but also in applying them in a cellular environment. Graphic abstract

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Section: Discussionmentioning

confidence: 99%

The role of the electrokinetic charge of neurotrophis-based nanocarriers: protein distribution, toxicity, and oxidative stress in in vitro setting

et al. 2021

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“…One of the options to immobilize peptides or proteins exploits the deposition of molecular layers that bind electrostatically to the implant surface. The mechanism consists of the alternate adsorption of opposite-charged polyelectrolytes, which are stabilized because of attraction forces, creating a self-assembled polyelectrolytic multilayer (PEM) [ 40 , 41 ]. As the combination of PEMs and biomolecules can imitate native ECM and furnish adequate osteoconductive properties, LBL has been used to functionalize titanium surfaces by immobilizing organic bioactive molecules [ 42 ].…”

Section: Techniques That Can Be Used To Produce Bmtismentioning

confidence: 99%

Biochemical Modification of Titanium Oral Implants: Evidence from In Vivo Studies

2021

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The discovery of osseointegration of titanium implants revolutionized the dental prosthesis field. Traditionally, implants have a surface that is processed by additive or subtractive techniques, which have positive effects on the osseointegration process by altering the topography. In the last decade, innovative implant surfaces have been developed, on which biologically active molecules have been immobilized with the aim of increasing stimulation at the implant–biological tissue interface, thus favoring the quality of osseointegration. Among these molecules, some are normally present in the human body, and the techniques for the immobilization of these molecules on the implant surface have been called Biochemical Modification of Titanium Surfaces (BMTiS). Different techniques have been described in order to immobilize those biomolecules on titanium implant surfaces. The aim of the present paper is to present evidence, available from in vivo studies, about the effects of biochemical modification of titanium oral implants on osseointegration.

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“…The immobilization of a higher number of enzymes may be achieved by embedding enzymes in a multilayered film using the sequential layer-by-layer (LbL) method, originally developed for polyelectrolytes [ 131 ]. This approach offers the possibility of incorporating biomacromolecules in thin films while preserving their bioactivity [ 132 ] and can be applied to almost all solid supports [ 133 ].…”

Section: Enzymatic Approachmentioning

confidence: 99%

Enzymatic Approach in Calcium Phosphate Biomineralization: A Contribution to Reconcile the Physicochemical with the Physiological View

Guibert

Landoulsi

2021

IJMS

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Biomineralization is the process by which organisms produce hard inorganic matter from soft tissues with outstanding control of mineral deposition in time and space. For this purpose, organisms deploy a sophisticated “toolkit” that has resulted in significant evolutionary innovations, for which calcium phosphate (CaP) is the biomineral selected for the skeleton of vertebrates. While CaP mineral formation in aqueous media can be investigated by studying thermodynamics and kinetics of phase transitions in supersaturated solutions, biogenic mineralization requires coping with the inherent complexity of biological systems. This mainly includes compartmentalization and homeostatic processes used by organisms to regulate key physiological factors, including temperature, pH and ion concentration. A detailed analysis of the literature shows the emergence of two main views describing the mechanism of CaP biomineralization. The first one, more dedicated to the study of in vivo systems and supported by researchers in physiology, often involves matrix vesicles (MVs). The second one, more investigated by the physicochemistry community, involves collagen intrafibrillar mineralization particularly through in vitro acellular models. Herein, we show that there is an obvious need in the biological systems to control both where and when the mineral forms through an in-depth survey of the mechanism of CaP mineralization. This necessity could gather both communities of physiologists and physicochemists under a common interest for an enzymatic approach to better describe CaP biomineralization. Both homogeneous and heterogeneous enzymatic catalyses are conceivable for these systems, and a few preliminary promising results on CaP mineralization for both types of enzymatic catalysis are reported in this work. Through them, we aim to describe the relevance of our point of view and the likely findings that could be obtained when adding an enzymatic approach to the already rich and creative research field dealing with CaP mineralization. This complementary approach could lead to a better understanding of the biomineralization mechanism and inspire the biomimetic design of new materials.

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Protein-based polyelectrolyte multilayers

Cited by 32 publications

References 108 publications

The role of the electrokinetic charge of neurotrophis-based nanocarriers: protein distribution, toxicity, and oxidative stress in in vitro setting

The role of the electrokinetic charge of neurotrophis-based nanocarriers: protein distribution, toxicity, and oxidative stress in in vitro setting

Biochemical Modification of Titanium Oral Implants: Evidence from In Vivo Studies

Enzymatic Approach in Calcium Phosphate Biomineralization: A Contribution to Reconcile the Physicochemical with the Physiological View

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