Protein‐Based Hydrogels with Tunable Dynamic Responses

Sui, Zhijie; King, William J.; Murphy, William L.

doi:10.1002/adfm.200701288

Cited by 88 publications

(91 citation statements)

References 35 publications

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“…[ 38,39 ] For silk protein based hydrogels, the β-sheet structure serves as crosslinking sites, which gives rise to the gel network formation and immobilize the molecular chain throughout the whole hydrogels. Our work illuminated that intermolecular β-sheet structure of RSF molecules may be essential for the hydrogel network, thus, the differences between β-sheet structure in the mechanically strong RSF/HPMC9 hydrogel and that of in pure RSF hydrogel should be focused.…”

Section: Size Of the Crosslinking Sites Of The Rsf/hpmc9 Hydrogelmentioning

confidence: 99%

Physically Crosslinked Biocompatible Silk‐Fibroin‐Based Hydrogels with High Mechanical Performance

Luo

Yang

Shao

2015

Adv Funct Materials

191

174

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have indeed shown greater mechanical performance than the traditional hydrogels. [8][9][10][11][12][13] However, problems still remained in terms of the applications of these hydrogels in biomedical fi elds. First, even if the elasticity of these hydrogels is improved signifi cantly, the modulus is far worse to meet the needs. Beyond that, poor biocompatibility as well as the possible toxicity of degradation product of these hydrogels poses a potential risk for the health of living beings. [ 6,7,14 ] Natural polymers, such as agarose, gelatin, hyaluronic acid and silk, possessing good biocompatibility, and the nontoxic degradation products, have received increasing interest in biomedical fi elds in the past decades. [ 15 ] Although these natural polymer based hydrogels have been extensively studied as the potential matrix for tissue engineering, the poor mechanical performance of the natural polymer based hydrogels is still a major limitation or a disadvantage for their medical applications. [ 16 ] Therefore, many methods were employed to improve the mechanical properties of the natural polymer based hydrogels. Chemical crosslinking was used as a facile routine approach to boost the mechanical properties of the natural polymer based hydrogels, but the safety of the chemical crosslinking reagent involved was a primary concern and the use of that might cause some unfavorable problems. [ 14 ] Besides chemical crosslinking, other efforts such as physical treatments [ 17,18 ] or mixing with other natural/synthetic polymers, [ 15,19 ] have been made to strengthen the natural polymer based hydrogels, yet the problem is still no closer to be solved.Among all natural polymers, Bombyx mori silk fi broin (SF) is one of the ideal candidates for biomedical applications due to it combining suffi cient mechanical performance, biocompatibility, and biodegradability. [20][21][22][23][24] Many approaches were employed to fabricate the physically crosslinked silk based hydrogels, such as sonication, vortex, addition of ethanol, and electrical fi eld. [25][26][27][28][29] However, most of the SF based hydrogels show poor mechanical performance which is a major stumbling block for applications. Recently, though a new covalently crosslinked SF based hydrogel with signifi cant elasticity was engineered via a kind of bio-crosslink reagent, i.e., HRP (horseradish peroxide), [ 7 ] it still could not escape the fate of poor mechanical properties in terms of the softness. Besides, the formation of the heterogeneous aggregates of crosslinking sites in the process of gelation can result in the poor optical properties Physically Crosslinked Biocompatible Silk-Fibroin-Based Hydrogels with High Mechanical PerformanceKunyuan Luo , Yuhong Yang , and Zhengzhong Shao * Developing hydrogel which combines superior mechanical performance and biocompatibility attracts researchers' attention in recent years. Here, a novel biocompatible hydrogel with excellent mechanical performance, comprised of regenerated silk fi broin (RSF) and hydroxypropyl methyl ce...

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Section: Size Of the Crosslinking Sites Of The Rsf/hpmc9 Hydrogelmentioning

confidence: 99%

Physically Crosslinked Biocompatible Silk‐Fibroin‐Based Hydrogels with High Mechanical Performance

Luo

Yang

Shao

2015

Adv Funct Materials

191

174

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“…Specifically, our approach relies on a ligand-induced protein conformational change to induce changes in hydrogel properties. In our general approach, which has been detailed previously, [5][6][7] In this study we formed and characterized dynamic hydrogel microspheres in which a protein conformational change was used to control microsphere volume changes and the release of an encapsulated drug. In particular, a specific biochemical ligand, trifluoperazine, induced calmodulin's nanometer scale conformation change, which translated to a 48.7% microsphere volume decrease.…”

Section: Introductionmentioning

confidence: 99%

“…PEG-CaM-PEG conjugates were synthesized, purified, and characterized as previously described. [5][6][7] PEG 575 -CaM-PEG 575 microspheres were formed using a water-in-oil emulsion polymerization in an Argon environment. The same process was repeated with a different water phase containing PEGDA 575 to form PEG 575 microspheres.…”

Section: Introductionmentioning

confidence: 99%

Triggered Drug Release from Dynamic Microspheres via a Protein Conformational Change

King¹,

Pytel²,

Ng³

et al. 2010

Macromolecular Bioscience

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In this study we formed and characterized dynamic hydrogel microspheres in which a protein conformational change was used to control microsphere volume changes and the release of an encapsulated drug. In particular, a specific biochemical ligand, trifluoperazine, induced calmodulin's nanometer scale conformation change, which translated to a 48.7% microsphere volume decrease. This specific, ligand‐induced volume change triggered the release of a model drug, vascular endothelial growth factor (VEGF), at pre‐determined times. After release from the microspheres, 85.6 ± 10.5% of VEGF was in its native conformation. Taken together, these results suggest that protein conformational change could serve as a useful mechanism to control drug release from dynamic hydrogels.magnified image

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“…In the presence of trifluoperazine, the CaM-containing hydrogels underwent a significant decrease in volume, demonstrating that a nanometer-scale protein conformational change can be translated into macroscopic changes in material properties. More recently, REVIEW www.advmat.de CaM-based materials have been photopolymerized to create materials that undergo substantial (more than five-fold), reversible volume changes, which can be used to synthesize spatially patterned actuators, [154] tunable optical biosensors, [173] and dynamic growth-factor delivery systems [174] (Fig. 8).…”

Section: Allosteric Protein Conformational Changes In Materialsmentioning

confidence: 99%

Bioinspired Design of Dynamic Materials

2009

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An emerging approach for design of dynamic materials involves mimicking natural systems, which are adept at changing their structure and function in response to their environment. Biological systems possess a diverse range of dynamic mechanisms, including competitive ligand–protein binding, enzyme‐catalyzed remodeling, and allosteric protein conformational changes. These dynamic mechanisms are now being exploited by materials scientists and engineers to design “bioinspired” synthetic materials that undergo responsive assembly and disassembly as well as dynamic volume and shape changes. The purpose of this review is to describe recent progress in design and development of bioinspired dynamic materials, with a particular emphasis on hydrogel networks. We specifically focus on emerging approaches that use biological phenomena as an inspiration for design of materials.

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Protein‐Based Hydrogels with Tunable Dynamic Responses

Cited by 88 publications

References 35 publications

Physically Crosslinked Biocompatible Silk‐Fibroin‐Based Hydrogels with High Mechanical Performance

Physically Crosslinked Biocompatible Silk‐Fibroin‐Based Hydrogels with High Mechanical Performance

Triggered Drug Release from Dynamic Microspheres via a Protein Conformational Change

Bioinspired Design of Dynamic Materials

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