Protein Antigen in Serotype <i>k Streptococcus mutans</i> Clinical Isolates

Nakano, Kazuhiko; Nomura, Ryota; Nemoto, Hirotoshi; Lapirattanakul, Jinthana; Taniguchi, Nobuaki; Grönroos, Lisa; Alaluusua, Satu; Ooshima, Takashi

doi:10.1177/154405910808701001

Cited by 26 publications

(35 citation statements)

References 24 publications

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“…Eight allele numbers for each strain defined the allelic profile and, consequently, sequence type (ST) of that strain. STs 1-108 were assigned in our previous studies (Lapirattanakul et al, 2008;Nakano et al, 2007). All new alleles defined in the present study are shown in bold in Table 2.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, internal fragments of eight housekeeping gene loci were amplified (Nakano et al, 2007), and their nucleotide sequences determined (Table 1). The sequences for each housekeeping gene fragment were compared with the previously described data (Lapirattanakul et al, 2008;Nakano et al, 2007) in order to assign allele numbers using a non-redundant database (http://linux.mlst.net/ nrdb/nrdb.htm). Eight allele numbers for each strain defined the allelic profile and, consequently, sequence type (ST) of that strain.…”

Section: Methodsmentioning

confidence: 99%

“…All allelic profiles determined in this project, together with our previous MLST data of 108 STs (Lapirattanakul et al, 2008;Nakano et al, 2007), were analysed with computational programs as follows. The START (sequence type analysis and recombinational tests) tools (http://pubmlst.org/software/analysis/start2) (Jolley et al, 2001) were used to produce a matrix of pairwise differences in the allelic profiles and a dendrogram was constructed from the matrix using UPGMA.…”

Section: Methodsmentioning

confidence: 99%

“…The Cnm protein possesses a collagen-binding domain (CBD) in its structure, which can bind to both collagen and laminin. Our previous studies have indicated the predominant frequency of this cnm gene in minor serotypes f and k, which usually showed unique characteristics concerning the pathogenesis of IE (Nakano et al, 2004a(Nakano et al, , 2007(Nakano et al, , 2008). An unambiguous typing method based on the nucleotide sequences of eight housekeeping genes, multilocus sequence typing (MLST), was developed for S. mutans, and showed a close evolutional relationship among cnmpositive strains (Nakano et al, 2007).…”

mentioning

confidence: 99%

“…Briefly, internal fragments of eight housekeeping gene loci were amplified (Nakano et al, 2007), and their nucleotide sequences determined (Table 1). The sequences for each housekeeping gene fragment were compared with the previously described data (Lapirattanakul et al, 2008;Nakano et al, 2007) in order to assign et al, 2001) were used to produce a matrix of pairwise differences in the allelic profiles and a dendrogram was constructed from the matrix using UPGMA. Then, related STs were grouped into lineages or clonal complexes using the eBURST (enhanced based upon related sequence types) program, version 3 (http://eburst.mlst.net) (Feil et al, 2004).…”

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confidence: 99%

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Multilocus sequence typing analysis of Streptococcus mutans strains with the cnm gene encoding collagen-binding adhesin

Lapirattanakul

Nakano

Nomura

et al. 2011

Journal of Medical Microbiology

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Streptococcus mutans is one of the oral pathogens associated with infective endocarditis (IE).With respect to bacterial binding ability to the extracellular matrix, the Cnm protein, a cell surface collagen-binding adhesin of S. mutans, is known as one of the possible virulence factors with regard to IE. In this study, we aimed to determine the distribution of the cnm gene, which encodes Cnm, in a large number of clinical isolates of S. mutans from Thai subjects. Then, the cnm-positive strains were classified using a multilocus sequence typing (MLST) scheme, which we constructed previously. In addition, the data were analysed together with our previous MLST data of cnmpositive strains from Japan and Finland in order to evaluate the clonal relationship among S. mutans strains harbouring the cnm gene. The cnm gene was detected in 12.4 % of all 750 Thai isolates, and serotype f showed the highest rate of detection (54.5 %). According to the MLST data, two clonal complex groups were revealed as the important clones related to cnm-positive S. mutans from various origins of isolation. Moreover, the collagen-binding properties of S. mutans strains with the cnm gene were significantly greater than those of strains without the gene, although four cnm-negative strains classified into two sequence types (STs), ST110 and ST136, showed extremely high collagen-binding rates suggesting the presence of additional genes involved with collagen binding in these STs. Taken together, these results provided information on both epidemiological as well as evolutional aspects of S. mutans possessing the cnm gene. INTRODUCTIONStreptococcus mutans is a well-known pathogen of dental caries (Hamada & Slade, 1980), which consists of four serotypes, c, e, f and k (Linzer et al., 1986;). The detection rate of serotype c is highest among the strains isolated from oral cavities, followed by serotype e, while serotypes f and k are regarded as being present in minor proportions . Various pieces of evidence show the association of S. mutans with some life-threatening diseases such as infective endocarditis (IE), although the major species related to IE belong to the mitis group of oral streptococci (Banas, 2004;Mylonakis & Calderwood, 2001). Moreover, continuous detections of this bacterium in specimens from patients suffering from such disease emphasize the importance of S. mutans in IE (Gauduchon et al., 2001;Nomura et al., 2006;Vose et al., 1987;Ullman et al., 1988). Indeed, studies to clarify specific components involved in IE in S. mutans have also been performed worldwide (Beg et al., 2002;Jung et al., 2009; MatsumotoNakano et al., 2009;Miller-Torbert et al., 2008).IE is initiated by the binding of bacterial surface molecules to host extracellular matrix (ECM) proteins exposed on damaged heart tissue (Moreillon & Que, 2004). In S. mutans, collagen-binding adhesin or Cnm protein, encoded by the cnm gene, was reported as a strain-specific collagen-binding molecule (Sato et al., 2004). The Cnm protein possesses a collagen-binding domain (CBD) in ...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

“…Briefly, internal fragments of eight housekeeping gene loci were amplified (Nakano et al, 2007), and their nucleotide sequences determined (Table 1). The sequences for each housekeeping gene fragment were compared with the previously described data (Lapirattanakul et al, 2008;Nakano et al, 2007) in order to assign et al, 2001) were used to produce a matrix of pairwise differences in the allelic profiles and a dendrogram was constructed from the matrix using UPGMA. Then, related STs were grouped into lineages or clonal complexes using the eBURST (enhanced based upon related sequence types) program, version 3 (http://eburst.mlst.net) (Feil et al, 2004).…”

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confidence: 99%

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