Protein analysis based on molecular beacon probes and biofunctionalized nanoparticles

Shi, Hui; He, Xiaoxiao; Yang, Xiaohai; Wang, Kemin; Wang, Qing; Guo, Qiuping; Huo, Xiqin

doi:10.1007/s11426-010-0110-3

Cited by 8 publications

(4 citation statements)

References 140 publications

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“…In comparison to other probes (such as linear DNA sequences), MBs with the same number of complementary nucleotides matching their target, are multitasking probes. They have advantages of thermodynamic [15], photostability owing to the reporter groups, flexible ability for conjugation, higher efficient intrinsic signal switching, ultra-sensitivity [15], and high selectivity [16]. Because of these impressive features, MBs have been successfully employed in a diversity of bioanalytical tenders that involve the recognition of DNA/RNA sequences [13,17,18].…”

Section: Introductionmentioning

confidence: 99%

Molecular beacon strategies for sensing purpose

Bidar

Amini

Oroojalian

et al. 2021

TrAC Trends in Analytical Chemistry

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Section: Introductionmentioning

confidence: 99%

Molecular beacon strategies for sensing purpose

Bidar

Amini

Oroojalian

et al. 2021

TrAC Trends in Analytical Chemistry

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“…In the presence of DNA polymerase, a single molecule is combined with the circular DNA (circ-DNA) and produce a long ssDNA (L-ssDNA), which has thousands of continuous complementary repeats of the circ-DNA sequence, which can be marked with fluorescence as the output signal, enabling the detection of targets at the single-molecule level . As a kind of fluorescent probe, the molecular beacon has become an important tool due to its extremely high specificity and sensitivity. − …”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Detection of 17β-Estradiol (E2) Based on Multistep Isothermal Amplification

Wang

Peng²,

Wu³

et al. 2021

Anal. Chem.

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17β-Estradiol (E2) can cause an adverse effect on the human endocrine system even at the nanomolar level. Measurements of very low levels of E2 remain a critical challenge due to insufficient sensitivity. In this study, a multistep isothermal amplification fluorescence strategy was constructed, which could realize the exponential amplification of target E2. Specifically, strand displacement reaction (SDA), rolling circle amplification (RCA), and multiprimed rolling circle amplification (MRCA) were combined in a series to quantify trace complementary strand of E2 (cDNA). The E2 aptamer and cDNA were hybridized and modified on the magnetic beads. E2 could bind to its aptamer and cause the release of the cDNA. Then, cDNA would combine with the template DNA, initiating the SDA−RCA−MRCA. The molecular beacons, possessing low background signal, whose fluorescence was quenched in the state of chain folding, could be specifically recognized by the long single-stranded DNA (L-ssDNA) generated by the multistep isothermal amplification triggered by cDNA, and then the fluorescence of the molecular beacons could be restored. Therefore, the E2 could be quantitatively detected by the recovery fluorescence intensity. The fluorescence value showed a good linear relationship with the concentration of E2 in the range of 0.001836−183.6 nM, and the limit of detection (LOD) was as low as 63.09 fM. In addition, the recovery rates of this method spiked in milk and water were 80.8−107.0%, respectively. This method has the advantage of multistep isothermal amplification to obtain abundant fluorescence signals, which may provide a new possibility for highly sensitive detection of other small-molecule targets.

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“…2,[27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] Other smaller molecules (proteins, antibodies, cofactors) require specialized conjugation to the SiNP. 38,[46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61] Even the addition of magnetic NP conjugation to the FSNP 3,4,62 greatly assists in the recognition and tracking of different organic molecules.…”

Section: Introductionmentioning

confidence: 99%