Protein amplification technology: New advances in human prion disease diagnosis

Application of α-Syn Real-Time Quaking-Induced Conversion for Brain and Skin Specimens of the Chinese Patients With Parkinson’s Disease

“…Real-time quaking-induced conversion technique, by inducing conversion of recombinant target proteins via intermittent shaking, has been widely evaluated and applied in the diagnosis of some neurodegenerative diseases, such as PrD and PD (Wu et al, 2021). In this study, we have validated the potential of the established α-Syn RT-QuIC on identification and amplification of trace amount of abnormal α-Syn in the brain and skin specimens of patients with PD.…”

“…High accuracies of PD diagnosis are obtained not only from skin tissues but also from many other CNS and peripheral tissues, such as brains, CSF, salivary gland, and colon (Bargar et al, 2021). As an easily obtained biopsy specimen, RT-QuIC of skin tissues has already showed more accessibility in the diagnosis of PD and PrD (Wang et al, 2019;Bargar et al, 2021;Wu et al, 2021;Xiao et al, 2021). Moreover, Manne et al (2020) have reported the usage of α-Syn RT-QuIC with formalin-fixed paraffin-embedded skin sections of PD cases with 75% of sensitivity and 83% of specificity, highlighting a much wide usage.…”