Protein-aggregating ability of different protoporphyrin-IX nanostructures is dependent on their oxidation and protein-binding capacity

Abstract

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Gallium Mesoporphyrin IX-Mediated Photodestruction: A Pharmacological Trojan Horse Strategy To Eliminate Multidrug-Resistant Staphylococcus aureus

“…Ga 3+ PPIX in 0.1 M NaOH titrated to PBS generated a double peak, which is most likely responsible for the appearance of oligomeric forms in the solution (Figure S1). The addition of 50% DMSO shifted the equilibrium of monomeric and oligomeric forms toward the one resulting in a higher absorption signal (Figure S1) and most likely corresponding to a monomer . Thus, the difference in the structure of both compounds (ethyl vs. vinyl groups) has a significant impact on their solubility in an aqueous solution.…”

“…The addition of 50% DMSO shifted the equilibrium of monomeric and oligomeric forms toward the one resulting in a higher absorption signal (Figure S1) and most likely corresponding to a monomer. 54 Thus, the difference in the structure of both compounds (ethyl vs. vinyl groups) has a significant impact on their solubility in an aqueous solution. This feature is extremely important from a clinical point of view.…”

Albumin Nanoparticles Loaded with Hemin as Peroxidase Mimics for Immunoassay**

“…These results demonstrate that hemin itself can stabilize BSA nanoparticles. Despite the ability of BSA to non‐covalently bind hemin is well known, [9] direct mixing of BSA with protoporphyrin‐IX does not cause protein aggregation [25] . Therefore, the mechanism of stabilization remains unclear.…”

Synthetic biology-instructed transdermal microneedle patch for traceable photodynamic therapy

“…administration of 5-ALA or CCPCA, especially in the brain (Fig. 8a, b), which may cause organelle-selective protein aggregation and phototoxicity to normal tissues as a higher concentration of PpIX than physiological condition 7,26,52 . Obvious FL signals of PpIX in both brain and normal skin tissues around tumors were also observed after i.t.…”