Protein adsorption on preadsorbed polyampholytic monolayers

Mahltig, Boris; Werner, Carsten; Müller, Martin; Jérôme, Robert; Stamm, Manfred

doi:10.1163/156856201753252525

Cited by 8 publications

(7 citation statements)

References 42 publications

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“…Our previous biosensor work has shown that these materials have excellent nonfouling characteristics only when the oppositely charged monomer subunits are present as a statistical copolymer mixture at the molecular level . Others have also completed surface-based investigations, confirming that polyampholyte polymer systems have nonfouling properties. − …”

Section: Introductionmentioning

confidence: 97%

Multifunctional Polyampholyte Hydrogels with Fouling Resistance and Protein Conjugation Capacity

Schroeder¹,

Zurick²,

McGrath³

et al. 2013

Biomacromolecules

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Materials that are resistant to nonspecific protein adsorption are critical in the biomedical community. Specifically, nonfouling implantable biomaterials are necessary to reduce the undesirable, but natural foreign body response. The focus of this investigation is to demonstrate that polyampholyte hydrogels prepared with equimolar quantities of positively charged [2-(acryloyloxy)ethyl] trimethylammonium chloride (TMA) and negatively charged 2-carboxyethyl acrylate (CAA) monomers are a viable solution to this problem. TMA/CAA hydrogels were prepared and their physical and chemical properties were characterized. The fouling resistance of the TMA/CAA hydrogels were assessed at varying cross-linker densities using enzyme-linked immunosorbant assays (ELISAs). The results clearly demonstrate that TMA/CAA hydrogels are resistant to nonspecific protein adsorption. A unique advantage of the fouling resistant TMA/CAA system is that bioactive proteins can be covalently attached to these materials using standard conjugation chemistry. This was demonstrated in this study through a combination of ELISA investigations and short-term cell adhesion assays. The multifunctional properties of the TMA/CAA polyampholyte hydrogels shown in this work clearly demonstrate the potential for these materials for use as tissue regeneration scaffolds for many biomedical applications.

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Section: Introductionmentioning

confidence: 97%

Multifunctional Polyampholyte Hydrogels with Fouling Resistance and Protein Conjugation Capacity

Schroeder¹,

Zurick²,

McGrath³

et al. 2013

Biomacromolecules

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“…Our previous work demonstrated that this concept could be applied to polyampholyte statistical copolymer brushes. These polymer brush coatings have been demonstrated to be nonfouling, as long as the individual charged units were homogeneously mixed at the molecular level. − This approach has since been adapted by others in additional polymer brush systems. − Similarly, investigators have examined polyampholyte polymer coatings that are adsorbed to surfaces rather than being grafted from the surface as an alternative mechanism for creating nonfouling surface coatings. , …”

Section: Introductionmentioning

confidence: 99%

“…28−30 Similarly, investigators have examined polyampholyte polymer coatings that are adsorbed to surfaces rather than being grafted from the surface as an alternative mechanism for creating nonfouling surface coatings. 31,32 Polyampholyte hydrogels and microgels have also been widely investigated as temperature and/or pH responsive systems useful for carrying and delivering drugs and proteins. 33−36 Given this application, it is often desirable for the hydrogel system to have protein adsorption capabilities.…”

Section: Introductionmentioning

confidence: 99%

Nonfouling Hydrogels Formed from Charged Monomer Subunits

2012

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A critical challenge in the field of biomaterials is the often undesirable, but immediate, coating of implants with nonspecifically adsorbed proteins upon contact with bodily fluids. Prior research has shown that overall neutral materials containing a homologous arrangement of mixed charges exhibit nonfouling properties. This has been widely demonstrated for zwitterionic materials and more recently for coatings containing an equimolar mixture of positively and negatively charged monomer subunits. In this investigation it is demonstrated that nonfouling hydrogels can be formed through this approach, and the physical properties of the resulting materials are thoroughly characterized. In particular, hydrogels were formed from mixtures of [2-(methacryloyloxy)ethyl]trimethylammonium chloride (TM) and 3-sulfopropyl methacrylate potassium salt (SA) monomers with varying concentrations of a triethylene glycol dimethacrylate (TEGDMA) cross-linker. The swelling, weight percentage water, surface zeta potential, and compressional properties of the gels were characterized, and the nonfouling properties were demonstrated using enzyme-linked immunosorbant assays for both negatively charged fibrinogen and positively charged lysozyme. The results confirm that the TM:SA hydrogel systems have nonfouling properties that are equivalent to established nonfouling controls. Additionally, even though the gels were resistant to nonspecific protein adsorption, a composition analysis suggests that there is room to further improve the nonfouling performance because there is a slight enrichment of the SA monomer relative to the TM monomer.

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“…This article will report the desorption behaviour of a diblock polyampholyte PMAA-b-PDMAEMA, poly(metha-crylic acid)-block-poly((dimethylamino)ethyl methacrylate), The used diblock system with a molecular weight of 15000 g/mol and a block ratio of PMAA: PDMAEMA 33 :67 is well characterized and known to adsorb in highly regular adsorbed structures [32]. Previous studies report also on the high stability of these polyampholyte layers under treatment with phosphate buffered protein solutions [33]. High adsorbed amounts of around 16 mg/m 2 on silicon substrates and regular adsorbed structures facilitate investigations on the desorption by ellipsometry and atomic force microscopy (AFM).…”

Section: Introductionmentioning

confidence: 99%

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Mahltig

Jérôme

Pfütze

et al. 2003

Journal of Polymer Research

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The desorption behaviour of the diblock polyampholyte PMAA-b-PDMAEMA, poly(methacrylic acid)-block-poly((dimethylamino)ethyl methacrylate), preadsorbed on silicon substrates was investigated under the influence of several desorption agents. The investigated polyampholyte is known to adsorb in regular structures directly from aqueous solutions onto silicon substrates While the adsorption process is mainly determined by electrostatic interactions, two kinds of desorp-tion mechanism should be assumed The first mechanism is based on changed electrostatic conditions caused for instance by a strong change in pH of the aqueous solution. The other mechanism is observed after treatment with hydrophobic organic solvent, which leads to the desorption of hydrophobic adsorbed polyampholyte chains, while the electrostatically attached ones will not be influenced. To complete the desorption experiments with organic solvents also adsorption experiments from analogous polyampholytic solutions in the same organic solvents were performed. The amount of polymer at the substrate surface after adsorption or desorption experiments was determined using ellipsometry. Atomic force microscopy (AFM) was used to investigate the surface topography of dried samples after the desorption process.

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Protein adsorption on preadsorbed polyampholytic monolayers

Cited by 8 publications

References 42 publications

Multifunctional Polyampholyte Hydrogels with Fouling Resistance and Protein Conjugation Capacity

Multifunctional Polyampholyte Hydrogels with Fouling Resistance and Protein Conjugation Capacity

Nonfouling Hydrogels Formed from Charged Monomer Subunits

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