Protein adsorption from seawater onto solid substrata: II. Behavior of bound protein and its influence on interfacial properties

Taylor, Gordon T.; Troy, Paul J.; Nullet, Michael A.; Sharma, Shiv K.; Liebert, Bruce E.

doi:10.1016/0304-4203(94)90011-6

Cited by 11 publications

(2 citation statements)

References 38 publications

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“…Because denatured proteins will disengage from the chamber wall after a period of time, chamber walls can become a constant sink for medium proteins. 31 Depletion of medium protein concentrations through protein adsorption affects subsequent culture conditions. In traditional culture, the relatively small surface area does not cause drastic alterations of medium by protein denaturing.…”

Section: Surface Adsorptionmentioning

confidence: 99%

Microenvironment design considerations for cellular scale studies

2004

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In vivo cellular microenvironments are not well-mimicked in present in vitro cell culture systems. Microtechnology, and microfluidics in particular, provides the tools to create in vivo-like cellular microenvironments in vitro. Features of in vitro cellular microenvironments are discussed and compared to macroscale cell culture environments; the concept of an effective culture volume (ECV) is introduced to facilitate the comparison. Current research using microtechnology to investigate in vitro cellular microenvironments is presented and areas where more research is needed in characterizing the in vitro microenvironment are outlined.

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Section: Surface Adsorptionmentioning

confidence: 99%

Microenvironment design considerations for cellular scale studies

2004

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“…Our experiments obviously do not model the chemical complexity of natural colloids, submicron particles and adsorbed organlc matter, but they do model adequately what is probably the most important mechanism, hydrophobic interactions (Kirchman et al 1989, Taylor et al 1994, by which organic matter adsorbs to surfaces in seawater. Furthermore, our results illuminating biochemical interactions between bacterial pro-teases (or protein-binding proteins) and adsorbed protein should hold even for hydrophilic submicron particles.…”

Section: Marine Colloids and Submicron Particles Providementioning

confidence: 99%

Bacterial degradation of protein adsorbed to model submicron particles in seawater

Nagata¹,

Dl²

1996

Mar. Ecol. Prog. Ser.

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We tested the hypothesls that proteln adsorbed to submlcron part~cles In seawater IS more slowly degraded than the same proteln freely d~ssolved Bacterial hydrolysis of methyl-3H-bov~ne serum a l b u m~n ( 3 H -B S~) dlssolved or adsorbed to particles (polystyrene latex beads diameters 0 126 to 1 5 pm) was examlned Mlxed bactenal assemblages cultured on BSA media, 2 bactenal ~solates, and a natural marlne bactelial assemblage hydrolyzed adsorbed 'H-BSA at much slower rates (1/10 to <1/200] than free 'H-BSA We compared our hydrolysls rates wlth the predicted collision probabilities between bactena and 3 H -B S~ freely dlssolved or adsorbed to beads of different diameters These comparisons suggest that the hydrolysls rate is ~nfluenced pnmanly by the transport rate of 'k-I-BSA to bacteria Hydrolysis of adsorbed 3 H -B S~ differed greatly between 2 bactenal strains whlch appears to be explained by differences In the afflnity of bactenal proteases to 3 H -B S~ OUI results support the hypothesls that adsorption onto subm~cron particles greatly reduces degradation rate of protelns and suggest thdt the adsorption of dissol\ied organic matter (DOM) may be a mechanism that temporarily stores lablle DOM in a slowly degrading, seml-labile pool, whlch makes 11 available for export and further modification by geochem~cal processes KEY WORDS-Bacterial degradation Dissolved organlc matter . Adsorption . Collolds INTRODUCTIONThe recent discovery of many detrital colloids and submicron particles in a wide variety of oceanic environments (Koike et al. 1990, Wells & Goldberg 1991 has renewed interests in the role of colloids in biogeochemical cycles of manne environments. These non-living organic collo~ds are much mol-e abundant (10"o 10'' particles ml-l, depending on size) than microorganisms including bacteria and viruses (Ko~ke et al. 1990, Wells & Goldberg 1991) and can represent a significant fraction (10 to 35%) of the organic carbon pool in the oceans (Benner et al. 1992). The chemical nature, sources, sinks, and turnover of colloids have yet to be clarified (Lee & Wakeham 1992, Nagata & Koike 1995).One potentially important role of colloids and submicron particles in oceanic material cycling is that they 'Present address Ocean Research Institute, The University of Tokyo 1-15-1 Mlnami-dal, Tokyo 164, Japan E-mail nagata@ori U-tokyo ac ]p provide large solid surfaces to which dissolved organic matter (DOM) may adsorb. In fact, prevlous studies have shown that adsorption of polymeric DOM such as protein to surfaces in seawater can occur very rapidly (within minutes; Kirchman et al. 1989) and that the adsorption can have substantial effects on degradation rates of organic material (van Loosdrecht et al. 1990, Fletcher 1991). Keil & Kirchman (1994 suggested that DOM adsorbed to colloids was less easily degraded than freely dissolved DOM. They found that dissolved protein aged in filtered seawater 1s abiotically modified to less b~ologically degradable protein in a short period (110 h), an observation that they could ex...

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Role of salts on BSA adsorption on stainless steel in aqueous solutions. I. FT‐IRRAS and XPS characterization

Pradier

Costa

Rubio

et al. 2002

Surface & Interface Analysis

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The understanding of protein interaction with metal surfaces has become a key question for the use of materials in medicine, in the food industry or in marine environment in connection with the process of biofouling. However, the role of the various salts present in natural seawater has never been systematically investigated. In the present work, we studied the adsorption of a protein, bovine serum albumin (BSA), on stainless-steel surfaces in artificial seawater and in aqueous solutions containing NaCl, NaCl + CaCl 2 and NaCl + MgCl 2 , the total concentration of salts being identical to that of the natural seawater (36 g l −1 ); the BSA concentration was fixed at 20 mg l −1 . After rinsing and drying, the surface was analysed by Fourier transform infrared reflection-adsorption spectroscopy (FT-IRRAS) and x-ray photoelectron spectroscopy (XPS). After immersion in aqueous solutions containing NaCl or NaCl + CaCl 2 and rinsing in saline solutions, the amount of adsorbed BSA is small. It increases by almost one order of magnitude if the immersion is performed in NaCl + MgCl 2 aqueous solution; after 24 h of immersion it reaches a saturation value very close to that attained in natural seawater. At saturation of the stainless-steel surface by the protein, the XPS metal signals were still detected, suggesting that the protein layer is heterogeneous, leaving patches of the surface almost uncovered. The amount of adsorbed BSA is reduced by almost one order of magnitude when the samples are rinsed in pure, demineralized water due to the dissolution of Mg 2+ cations and the elimination of loosely adsorbed proteins. The presence of Mg 2+ cations within the BSA layer is confirmed by angle-resolved XPS analyses.

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Protein adsorption from seawater onto solid substrata: II. Behavior of bound protein and its influence on interfacial properties

Cited by 11 publications

References 38 publications

Microenvironment design considerations for cellular scale studies

Microenvironment design considerations for cellular scale studies

Bacterial degradation of protein adsorbed to model submicron particles in seawater

Role of salts on BSA adsorption on stainless steel in aqueous solutions. I. FT‐IRRAS and XPS characterization

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