Protein Adsorption by Artificial Membrane Materials Under Filtration Conditions

Birk, Horst‐Walter; Kistner, Andrea; Wizemann, V.; Schütterle, G

doi:10.1111/j.1525-1594.1995.tb02351.x

Cited by 31 publications

(20 citation statements)

References 14 publications

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“…Birk et al observed that one type of polysulfone (Fresenius F60) showed a higher adsorption of both total proteins and low molecular weight proteins than another polysulfone membrane tested (D30) during an “in vitro” comparison of 11 different membrane materials [15]. Interestingly, Lamy et al enrolled 31 chronic hemodialysis patients in a prospective observational study evaluating the reduction of free light chain values with high-flux dialyzers like FX80 and FX100 [15]. The RRs for k free light chains was 66% with online HDF vs. 52% with bicarbonate dialysis ( p < 0.001), whereas no difference was found between the two techniques tested for λ free light chains (37% RRs with both techniques) [16].…”

Section: Discussionmentioning

confidence: 99%

Removal of free light chains in hemodialysis patients without multiple myeloma: a crossover comparison of three different dialyzers

Donati¹,

Moretti²,

Baraldi³

et al. 2016

BMC Nephrol

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BackgroundImmunoglobulin light chains are classified as middle molecule uremic toxins able to interact with B lymphocyte membranes leading to the activation of transmembrane signaling. The ensuing impairment of neutrophil function can contribute to the chronic inflammation state of uremic patients, and the increased risk of bacterial infections or vascular calcifications. The aim of this crossover observational study was to assess the difference in free light chain removal by three different hemodialysis filters in patients not affected by multiple myeloma.MethodsFree light chain removal was compared in the polymethylmethacrylate (PMMA) membrane Filtryzer BK-F, the polyphenylene HFR17 filter and the conventional polysulfone filter F7HPS. Twenty chronic hemodialysis patients were enrolled: mean age was 67.7 ± 17.0 years, M/F = 14/6, dialysis vintage (months) 25.5 ± 32.0. The patients were randomized into two groups of treatment lasting 6 weeks each. The dialysis sessions checked were the midweek sessions and the blood was drawn at times 0, 120’ and 240’. Kappa (k) and lambda (λ) light chain levels, β2microglobulin (β2M), C reactive protein (CRP) and albumin were checked.ResultsK light chain levels were 345.0 ± 100.0 mg/L, λ light chains were 121.4 ± 27.0 mg/L. The values of k light chains at times 120’ and 240’ were significantly lower with PMMA and HFR17 than those obtained with F7. The reduction ratio per session (RRs) for k light chains was 44.1 ± 4.3% with HFR17, 55.3 ± 3.4% with PMMA, 25.7 ± 8.3% with F7 (p = 0.018). The RRs for λ light chains was 30.3 ± 2.9% with HFR17, 37.8 ± 17.3% with PMMA, 14.0 ± 3.9% with F7 (p = 0.032). As to β2M, RRs was 42.4 ± 3.2% with HFR17 vs. 33.9 ± 2.8% with PMMA vs. 6.3 ± 1.9% with F7 (p = 0.022). The three filters tested showed no differences in CRP or albumin levels.ConclusionIn terms of light chain and β2M removal, the PMMA and on-line HFR filters are similar and both are significantly more effective than the F7 filter in chronic dialysis patients.Trial registrationThe present trial was registered retrospectively (NCT02950389, 31/10/2016).

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Section: Discussionmentioning

confidence: 99%

Removal of free light chains in hemodialysis patients without multiple myeloma: a crossover comparison of three different dialyzers

Donati¹,

Moretti²,

Baraldi³

et al. 2016

BMC Nephrol

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“…BK-H and B1, which have the larger pore sizes among the PMMA membranes, remove not only BMG but also intact PTH (iPTH) as compared with other membranes. There have been successive reports on the general adsorptive removal of proteins by PMMA membranes [18][19][20]. Figure 3 shows Aoike's data [19], a twodimensional electrophoresis pattern of samples from plasma of a hemodialysis patient and a normal subject.…”

Section: Removal Of Various Protein Components By Adsorptionmentioning

confidence: 99%

Polymethylmethacrylate Membrane with a Series of Serendipity

Sakai

2011

Contributions to Nephrology

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Forty years have passed since the polymethylmethacrylate (PMMA) membrane was first developed. This article reviews its history and explains its longevity. The membrane was developed through application of a stereocomplex phenomenon that is observed upon mixture of isotactic and syndiotactic PMMA polymers. Filtryzer(TM) B1 and B2 were approved in Japan in 1977. B1 was the pioneer high-performance membrane model and B2 was a model that simulated a low-flux cellulosic membrane. The development of B1 led to the development of the dialysis machine with an ultrafiltration rate (UFR)-controlling function because the UFR of B1 was too high to control using transmembrane pressure control. B1 was used not only as a dialyzer but also as a hemodiafilter by combination with a UFR controller. Biocompatibility of the dialysis membrane, complement activation and/or transient leukopenia was studied with B2. Cooperative studies between Niigata University and Toray resulted in Gejyo's finding regarding the harmfulness of β(2)-microglobulin (BMG). Long-term follow-up of patients dialyzed using the BK membrane revealed that plasma BMG levels were significantly low and that the occurrence ratio of carpal tunnel syndrome was suppressed. These results were obtained by the adsorptive removal of BMG onto a PMMA membrane. Several papers have discussed new aspects in succession mainly based on clinical experiences that were not aimed at a development stage, i.e. they were kinds of serendipity. For the BK-F membrane with the largest pore size, this includes anemia and removal or modification of furancarboxylic acid, homocysteine, pentosidine and soluble CD40. For the BG membrane with a slightly anionic component, this includes pruritus and removal of free immunoglobulin light chains. Even patients' prognoses may be modified by the use of PMMA membrane. The mechanisms of these findings have been clarified bit by bit and the membrane will further open new frontiers in dialysis treatment.

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“…Previously, membrane adsorption on the polysulfone membrane was investigated using 2-D gel electrophoresis [15]. Because the dominant peaks (6,629 and 6,431) adsorbed onto the polysulfone membrane in the area of less than m/z 11,730 were identified as ApoCI with a pI value of 7.9 and truncated ApoCI with a pI value of 8.2 in this study, spots with a low molecular weight and pH 8–10 on 2-D electrophoresis may be ApoCI and truncated ApoCI.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Serum, Outflow Dialysate and Adsorbed Protein onto Dialysis Membranes (Polysulfone and PMMA) during Hemodialysis Treatment Using SELDI-TOF-MS

et al. 2006

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Aims: Alterations in the profiling of peptides and proteins in the serum, outflow dialysate and adsorbed protein on the dialysis membrane were investigated. Methods: Alterations in the protein profiling of routine hemodialysis using polysulfone (TS-UL) and PMMA (moderate flux membrane of polymethylmethacrylate: BK-U) in 8 patients and that of adsorption onto polysulfone and PMMA membranes in 4 patients were evaluated by SELDI-TOF-MS and ProteinChip array. Mass-to-charge ratios (m/z) between 2,000 and 120,000 were analyzed. Results: The protein with a relative intensity of m/z 11,730 measured by SELDI-TOF-MS was present in a small amount in the outflow dialysate and in a large amount in adsorption (identified as β₂-microglobulin) onto PMMA membrane. Unexpectedly, 68 molecular masses of peptides that were adsorbed more onto polysulfone than onto PMMA membrane were observed. There were more peptides less than m/z 11,730 adsorbed onto polysulfone membrane than onto PMMA membrane. Dominant peaks, m/z 6,629 and 6,431 adsorbed onto polysulfone membrane were identified as apolipoprotein CI and truncated apolipoprotein CI, respectively. 37 proteins with molecular weights larger than m/z 11,730 showed greater filtration through PMMA membrane than through polysulfone membrane. 149 molecular masses that were adsorbed onto PMMA or more onto PMMA membrane than onto polysulfone membrane were observed. Conclusion: This experiment suggests that membrane adsorption is an important mechanism for the removal of middle-molecular-weight proteins by hemodialysis using not only PMMA membrane but also polysulfone membrane. Adsorption of peptide or protein onto a dialysis membrane may depend not only on the membrane material, but also on the peptide or protein.

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Protein Adsorption by Artificial Membrane Materials Under Filtration Conditions

Cited by 31 publications

References 14 publications

Removal of free light chains in hemodialysis patients without multiple myeloma: a crossover comparison of three different dialyzers

Removal of free light chains in hemodialysis patients without multiple myeloma: a crossover comparison of three different dialyzers

Polymethylmethacrylate Membrane with a Series of Serendipity

Proteomic Analysis of Serum, Outflow Dialysate and Adsorbed Protein onto Dialysis Membranes (Polysulfone and PMMA) during Hemodialysis Treatment Using SELDI-TOF-MS

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