Proteomic Analysis of Serum, Outflow Dialysate and Adsorbed Protein onto Dialysis Membranes (Polysulfone and PMMA) during Hemodialysis Treatment Using SELDI-TOF-MS

Abstract

Aims: Alterations in the profiling of peptides and proteins in the serum, outflow dialysate and adsorbed protein on the dialysis membrane were investigated. Methods: Alterations in the protein profiling of routine hemodialysis using polysulfone (TS-UL) and PMMA (moderate flux membrane of polymethylmethacrylate: BK-U) in 8 patients and that of adsorption onto polysulfone and PMMA membranes in 4 patients were evaluated by SELDI-TOF-MS and ProteinChip array. Mass-to-charge ratios (m/z) between 2,000 and 120,000 were analyzed. Results: The protein with a relative intensity of m/z 11,730 measured by SELDI-TOF-MS was present in a small amount in the outflow dialysate and in a large amount in adsorption (identified as β₂-microglobulin) onto PMMA membrane. Unexpectedly, 68 molecular masses of peptides that were adsorbed more onto polysulfone than onto PMMA membrane were observed. There were more peptides less than m/z 11,730 adsorbed onto polysulfone membrane than onto PMMA membrane. Dominant peaks, m/z 6,629 and 6,431 adsorbed onto polysulfone membrane were identified as apolipoprotein CI and truncated apolipoprotein CI, respectively. 37 proteins with molecular weights larger than m/z 11,730 showed greater filtration through PMMA membrane than through polysulfone membrane. 149 molecular masses that were adsorbed onto PMMA or more onto PMMA membrane than onto polysulfone membrane were observed. Conclusion: This experiment suggests that membrane adsorption is an important mechanism for the removal of middle-molecular-weight proteins by hemodialysis using not only PMMA membrane but also polysulfone membrane. Adsorption of peptide or protein onto a dialysis membrane may depend not only on the membrane material, but also on the peptide or protein.

Specific adsorption of some complement activation proteins to polysulfone dialysis membranes during hemodialysis

“…[31][32][33][34][35] or clusterin (spot nos. [23][24][25]. The protein identifications were further verified by the comparison of measured pI and MW (estimated by the coordinates of the spots in gel) with their theoretical values (as predicted by the protein database).…”

β2-Microglobulin as a Biomarker in Peripheral Arterial Disease

“…These peaks were putatively identified as representing B2M. 18,19 Other peaks found to be higher in the PAD group were 13-and 15-kDa proteins putatively identified as cystatin C and lysozyme C, respectively. Furthermore, unidentified proteins at 22 and 36 kDa were increased in the PAD group.…”

Cytokine-adsorbing hemofilter: old but new modality for septic acute kidney injury

“…A subsequent investigation revealed that the β 2 -MG level in the dialysate was extremely low when a PMMA dialyzer (BK Series®) was used for hemodialysis and that this phenomenon was due to adsorption of β 2 -MG to PMMA membrane [27]. Another subsequent investigation demonstrated that β 2 -MG was detectable in the filtrate after the adsorption capacity of the PMMA dialyzer reached saturation level over time [28].…”