Protein adsorption and surface patterning

Ekblad, Tobias; Liedberg, Bo

doi:10.1016/j.cocis.2010.07.008

Cited by 76 publications

(72 citation statements)

References 95 publications

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“…[5]. Adsorption on hydrophilic silicon surfaces is related to the electrostatic interaction between the protein and the surface [6,7] and thus depends on the charge of the protein which itself varies with the isoelectric point (PI) and pH [8][9][10][11]. Van der Waals interactions may also have non-negligible effects on the adsorption, as illustrated recently [12].…”

Section: Introductionmentioning

confidence: 99%

A neutron reflection study of adsorbed deuterated myoglobin layers on hydrophobic surfaces

Brouette

Fragneto²,

Cousin

et al. 2013

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

A neutron reflection study of adsorbed deuterated myoglobin layers on hydrophobic surfaces

Brouette

Fragneto²,

Cousin

et al. 2013

Journal of Colloid and Interface Science

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“…Previous work has investigated various surface modifications strategies to alter the hemocompatibility of biomaterial surfaces [23–25]. Inorganic and organic coatings [26], polymer surface chemical modification [27], and chemically patterned surfaces [28] have been used to alter hemocompatibility. These surfaces have proven to produce favorable hemocompatible response through inertness, chemical and mechanical stability, and low protein adsorption [19, 28].…”

Section: Introductionmentioning

confidence: 99%

Hemocompatibility of polymeric nanostructured surfaces

Leszczak

Smith

Popat

2013

Journal of Biomaterials Science, Polymer Edition

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Tissue integration is an important property when inducing transplant tolerance, however, the hemocompatibility of the biomaterial surface also plays an important role in the ultimate success of the implant. Therefore, in order to induce transplant tolerance, it is critical to understand the interaction of blood components with the material surfaces. In this study, we have investigated the adsorption of key blood serum proteins, in vitro adhesion and activation of platelets and clotting kinetics of whole blood on flat polycaprolactone (PCL) surfaces, nanowire (NW) surfaces and nanofiber (NF) surfaces. Previous studies have shown that polymeric nanostructured surfaces improve cell adhesion, proliferation and viability; however it is unclear how these polymeric nanostructured surfaces interact with the blood and its components. Protein adsorption results indicate that while there were no significant differences in total albumin adsorption on PCL, NW and NF surfaces, NW surfaces had higher total fibrinogen and immunoglobulin-G adsorption compared to NF and PCL surfaces. In contrast, NF surfaces had higher surface FIB and IgG adsorption compared to PCL and NW surfaces. Platelet adhesion and viability studies show more adhesion and clustering of platelets on the NF surfaces as compared to PCL and NW surfaces. Platelet activation studies reveal that NW surfaces have the highest percentage of unactivated platelets, whereas NF surfaces have the highest percentage of fully activated platelets. Whole blood clotting results indicate that NW surfaces maintain an increased amount of free hemoglobin during the clotting process compared to PCL and NF surface, indicating less clotting and slower rate of clotting on their surfaces.

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“…First of all, the active biomolecule (protein, antibody or nucleic acid) must be immobilised onto the selected surface at the appropriate surface density while maintaining its activity. The way they are immobilised (physical adsorption Ekblad & Liedberg, 2010;Kidoaki & Matsuda, 2002;Nakanishi et al, 2001) versus covalent binding (Brady & Jordaan, 2009;Frasconi et al, 2010;Gandhiraman et al, 2009;Stutz, 2009)) and the surface substrate properties (wettability, roughness… (Gandhiraman et al, 2010a)) are, amongst others, parameters affecting biomolecule density and activity. Also, once proteins are attached to surfaces, different factors are affecting their biomolecular recogition (specific binding), i.e.…”

Section: Introductionmentioning

confidence: 99%