Protein adsorption and separation with chitosan-based amphoteric membranes

Novel ultrafiltration membranes with adjustable charge density based on sulfonated poly(arylene ether sulfone) block copolymers and their tunable protein separation performance

“…The pH of protein solution plays an important role in UF separations using charged membranes [6,7,14,19,30,48]. The electrical charge on the proteins and membranes can be…”

“…The effect of solution pH on the adsorption of protein resulted from the change in the electrostatic potential of the protein and the membrane[6,7,14,30,48]. The net charge on BSA changed from positive to negative when solution pH was changed from 3 to 7; while the membrane surface remained at about the same negative value in this pH range (cf.…”

Adsorption of Cellulase Isolated fromAspergillus Nigeron Chitosan/Alginate Particles Functionalized with Epichlorohydrin

“…Even though these biopolymers have been widely reported individually for purification of bioproducts (Shi et al 2003;Hoven et al 2007;Torres et al 2007;Feng et al 2009;Xu et al 2009), very few records exist regarding their combined use for biomolecule adsorption. In the case of cellulase, to our knowledge, there are no such studies to recover the enzyme, apart from conventional salt precipitation techniques and ion-exchange or gel permeation chromatography using state-of-the-art polymeric resins (Avelino et al 1999;Roy et al 1999;Amritkar et al 2004;Mirzaakhmedov et al 2007;Wang et al 2009).…”

Adsorption kinetic character of copper ions onto a modified chitosan transparent thin membrane from aqueous solution

“…Heavy metal ions can be removed when the ions are in contact with the surface of the membrane, even when the pore sizes of the membrane are much lager than the dimensions of the metal ions. Adsorptive adsorbents prepared by polymer chitosan (CS) have attracted obvious attentions in biomedical or biochemical field for separation and purification, and in the environmental field for the removal of heavy metal ions, such as the removal of protein [4,10], the removal of dyestuffs [11,12], and adsorption of heavy metal ions [3,9,[13][14][15][16]. Chitosan is a heteropolymer with high content of amine (-NH 2 ) functional group and remarkable availability.…”