Protein adsorption and inactivation on surfaces. Influence of heterogeneities

Chemical Characterization of Organic Carbon Dissolved in Natural Waters Using Inorganic Adsorbents

“…The isoelectric point of BSA is 4.7 -5.0, 33 and because most proteins are less than 7, they may well have high adsorptivity to SG under acidic conditions. Sadana 33 interpreted that in addition to the electrostatic interaction, protein adsorption was due to a decrease in the conformational stability of a protein with increasing net charge on the molecule. As the pH is far from the protein's isoelectric point, a structural rearrangement occurs and the rearranged molecules have larger surface areas than the original molecules, and thus the adsorption rates decrease.…”

The Use of Chromatography to Manufacture Purer and Safer Plasma Products

“…What do we know about viruses then that can help predict chromatographic interactions? We know that electrostatic forces steer the protein towards the charged resin (Sadana, 1992). We know that viruses are generally negatively charged at neutral pH, and it has been frequently noted viruses will bind to some filter membranes as a result of electrostatic attraction (Brock, 1983).…”

Closing the gap: accelerating the translational process in nanomedicine by proposing standardized characterization techniques

“…Almost all administration routes available for nanocarriers require them to be in aqueous solution; hence, it is imperative to determine the hydrodynamic size of a nanocarrier, which changes once it is in the bloodstream due to the phenomenon of adsorption and desorption of proteins. 7 Consequently, physicochemical characterization of the nanocarrier before, during, and after administration is essential. A summary of the key physicochemical characterization parameters of nanomaterials that interact with the immune system is also given in the second chapter of the "Handbook of Immunological Properties of Engineered Nanomaterials".…”

“…87 There has been much debate in the scientific literature regarding the occurrence and significance of the "Vroman effect", which postulates that proteins at lower concentrations in serum replace proteins at higher concentrations over time. 7,84,88,89 If this effect is indeed present with nanomaterials, it seems that the rate of desorption and adsorption is very fast, and beyond the detection limit of many of the kinetic experiments that have been performed. Newer nanomaterials with complex morphologies and physicochemical characteristics are rapidly being synthesized, so it is more relevant to consider the nanocarrier-protein interactions than to confirm the extent to which the Vroman effect is taking place.…”

“…Surface adhesion of nonpolar proteins results from both an interaction between different nonpolar groups and destruction of the water clathrate that forms around the protein. 7,106 Many peer-reviewed papers have discussed a change in the conformation of proteins as they undergo this adhesion process, which fundamentally affects their function in the blood. 84,98,108 Two studies were conducted using five types of latex particles with different surface chemistries, and found that each different arrangement of ionic charges or hydroxyl groups on the surface of the particle induced different types of protein adsorption.…”