Closing the gap: accelerating the translational process in nanomedicine by proposing standardized characterization techniques

Khorasani, Ali A.; Weaver, James L.; Salvador-Morales, Carolina

doi:10.2147/ijn.s72479

Cited by 12 publications

(5 citation statements)

References 116 publications

(209 reference statements)

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“…Nanomedicine is one of the most exciting fields of research in the branch of nanotechnology as it has the potential to generate practical and effective solutions to tackle chronic diseases and to solve unmet clinical challenges. However, a tremendous gap exists between the number of numerous formulation types synthesised in research laboratories and those approved for clinics [1], mainly due to the lack of understanding on the nanoparticles (NPs) behaviour in complex media that can affect their efficacy and their biocompatibility [2].…”

Section: Introductionmentioning

confidence: 99%

Identification of physicochemical properties that modulate nanoparticle aggregation in blood

Soddu¹,

Trinh²,

Dunne³

et al. 2020

Beilstein J. Nanotechnol.

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Inorganic materials are receiving significant interest in medicine given their usefulness for therapeutic applications such as targeted drug delivery, active pharmaceutical carriers and medical imaging. However, poor knowledge of the side effects related to their use is an obstacle to clinical translation. For the development of molecular drugs, the concept of safe-by-design has become an efficient pharmaceutical strategy with the aim of reducing costs, which can also accelerate the translation into the market. In the case of materials, the application these approaches is hampered by poor knowledge of how the physical and chemical properties of the material trigger the biological response. Hemocompatibility is a crucial aspect to take into consideration for those materials that are intended for medical applications. The formation of nanoparticle agglomerates can cause severe side effects that may induce occlusion of blood vessels and thrombotic events. Additionally, nanoparticles can interfere with the coagulation cascade causing both pro- and anti-coagulant properties. There is contrasting evidence on how the physicochemical properties of the material modulate these effects. In this work, we developed two sets of tailored carbon and silica nanoparticles with three different diameters in the 100–500 nm range with the purpose of investigating the role of surface curvature and chemistry on platelet aggregation, activation and adhesion. Substantial differences were found in the composition of the protein corona depending on the chemical nature of the nanoparticles, while the surface curvature was found to play a minor role. On the other hand, large carbon nanoparticles (but not small carbon nanoparticles or silica nanoparticles) have a clear tendency to form aggregates both in plasma and blood. This effect was observed both in the presence or absence of platelets and was independent of platelet activation. Overall, the results presented herein suggest the existence of independent modes of action that are differently affected by the physicochemical properties of the materials, potentially leading to vessel occlusion and/or formation of thrombi in vivo.

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Section: Introductionmentioning

confidence: 99%

Identification of physicochemical properties that modulate nanoparticle aggregation in blood

Soddu¹,

Trinh²,

Dunne³

et al. 2020

Beilstein J. Nanotechnol.

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“…14 In this review, we focus on the control and characterization of biomolecule adsorption from a liquid solution to a surface, either bare or functionalized. Thus, we will not address the case of nanoparticles in solution, 15 for which the analytical techniques are different. We are also not presenting techniques that are specific to the field of biosensing since here we do not aim to quantify the concentration of analytes in a solution but rather quantify the amount of adsorbed biomolecules on a given surface and how they interact with this surface (kinetics, conformational state).…”

Section: Tissue Engineeringmentioning

confidence: 99%

Comment on “Tuning the bioactivity of bone morphogenetic protein-2 with surface immobilization strategies” by Chen et al.

Cavalcanti‐Adam

Migliorini

2019

Acta Biomaterialia

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The control over the adsorption or grafting of biomolecules from a liquid to a solid interface is of fundamental importance in different fields, such as drug delivery, pharmaceutics, diagnostics, and tissue engineering. It is thus important to understand and characterize how biomolecules interact with surfaces and to quantitatively measure parameters such as adsorbed amount, kinetics of adsorption and desorption, conformation of the adsorbed biomolecules, orientation, and aggregation state. A better understanding of these interfacial phenomena will help optimize the engineering of biofunctional surfaces, preserving the activity of biomolecules and avoiding unwanted side effects. The characterization of molecular adsorption on a solid surface requires the use of analytical techniques, which are able to detect very low quantities of material in a liquid environment without modifying the adsorption process during acquisition. In general, the combination of different techniques will give a more complete characterization of the layers adsorbed onto a substrate. In this review, the authors will introduce the context, then the different factors influencing the adsorption of biomolecules, as well as relevant parameters that characterize their adsorption. They review surface-sensitive techniques which are able to describe different properties of proteins and polymeric films on solid two-dimensional materials and compare these techniques in terms of sensitivity, penetration depth, ease of use, and ability to perform "parallel measurements."

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“…There are three basic steps in the standard nanomedicine evaluation process: a planar cell culture model for evaluating potency and toxicity of nanomedicines; preclinical animal models such as mice or rats, for evaluating the release, absorption, interaction, pharmacokinetics, pharmacodynamics and clearance of nanomedicines in complex organisms; finally, clinical trials for evaluating the safety and efficacy of nanomedicine for patients [141,142]. This whole process is expensive and laborious and has high failure rates, leading to the slow clinical translation of nanomedicines [143,144].…”

Section: Organs-on-chips For Nanomedicine Evaluationmentioning

confidence: 99%

Evaluating nanomedicine with microfluidics

Ranganathan

2018

Nanotechnology

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Nanomedicines are engineered nanoscale structures that have an extensive range of application in the diagnosis and therapy of many diseases. Despite the rapid progress in and tremendous potential of nanomedicines, their clinical translational process is still slow, owing to the difficulty in understanding, evaluating, and predicting their behavior in complex living organisms. Microfluidic techniques offer a promising way to resolve these challenges. Carefully designed microfluidic chips enable in vivo microenvironment simulation and high-throughput analysis, thus providing robust platforms for nanomedicine evaluation. Here, we summarize the recent developments and achievements in microfluidic methods for nanomedicine evaluation, categorized into four sections based on their target systems: single cell, multicellular system, organ, and organism levels. Finally, we provide our perspectives on the challenges and future directions of microfluidics-based nanomedicine evaluation.

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Closing the gap: accelerating the translational process in nanomedicine by proposing standardized characterization techniques

Cited by 12 publications

References 116 publications

Identification of physicochemical properties that modulate nanoparticle aggregation in blood

Identification of physicochemical properties that modulate nanoparticle aggregation in blood

Comment on “Tuning the bioactivity of bone morphogenetic protein-2 with surface immobilization strategies” by Chen et al.

Evaluating nanomedicine with microfluidics

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