Protein Adhesion at Poly(ethylene glycol) Modified Surfaces

Snellings, Geert; Vansteenkiste, Stefan; Corneillie, Siska; Davies, Martyn C.; Schacht, E.

doi:10.1002/1521-4095(200012)12:24<1959::aid-adma1959>3.3.co;2-m

Cited by 19 publications

(26 citation statements)

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“…PEG is an ideal anti-fouling material with excellent segment mobility and strong hydrogen bonds in water environment, and numerous studies have demonstrated the excellent antifouling effect. [7][8][9] Because the linear backbone structure of C-O-C is easily oxidized and broken, it remains a challenge to ensure the persistent antifouling effect of PEG. On the other hand, the modication of separation membrane requires suitable implement method.…”

Section: Introductionmentioning

confidence: 99%

Enhanced separation and antifouling properties of PVDF ultrafiltration membranes with surface covalent self-assembly of polyethylene glycol

Zhao

Xuan

2015

RSC Adv.

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8The branched polyethylene glycol (PEG) was anchored onto membrane surface by 9 efficient covalent self-assembly to optimize the separation and antifouling properties of 10 polyvinylidene fluoride (PVDF) ultrafiltration membranes based on its excellent hydrophilic 11 and antifouling properties. The results showed that the surface self-assembly method could 12 effectively improve the stability of PEG and the surface wetting ability of PVDF membrane, 13 and simultaneously enhanced the water flux and rejection of modified membranes. 14 Meanwhile, the fouling results showed the anchored PEG segments on the separation 15 interface were contributed to improve the antifouling performance of PVDF ultrafiltration 16 membrane with significantly improved anti-adsorption capacity for bovine serum albumin 17 (BSA) and expectant water flux recover ratios for the BSA and humic acid (HA) two typical 18 pollutants. This paper provided a sample modification idea and demonstrated covalent 19 self-assembly was an effective method for surface modification. 20 21backbone structure of C-O-C is easily oxidized and broken, it remains a challenge to ensure 1 the persistent antifouling effect of PEG. On the other hand, the modification of separation 2 membrane requires suitable implement method. Blending as a common approach requires 3 specially designed and functional additives to ensure the compatibility with matrix 4 materials, 10,11 and the additives are easily lost in the separation process. Surface grafting is 5 still at the experimental stage due to the harsh reaction conditions and multi-step reaction 6 mode. The surface self-assembly technology is simple and easy to implement, and provides a 7 potential application with the desired effect. As we all know, the inefficiency is a 8 disadvantage for the practical application of self-assembly due to the dozens of assembly 9 layers. Furthermore, there is a big risk for the stability of assembled layer, charge and 10 hydrogen bond interactions will be destroyed easily in a violent environment. 12-14 As a 11 comparison, the covalent bonds are more stable, so the covalent self-assembly with less 12 assembly numbers will provide an efficient surface modification method with available 13 application.14 In this paper, covalent self-assembly technology is employed to modify the hydrophobic 15 surface of PVDF UF membranes by directed reaction of trimesoyl chloride (TMC) and PEG. 16The active sites are generated on the membrane surface with the aid of plasma technology to 17 introduce PEG layer by self-assembly at the separation interface, the physical and chemical 18 properties of the modified membrane surface were investigated, and the separation 19 performance and antifouling property of modified membranes were evaluated. This paper 20 aims to provide an efficient modification method to improve the interfacial physicochemical 21 property. 1 PEG-assembly number). The plasma treated PVDF membrane was named P-PVDF. 2 2.3 Characterization 3The chemical composition of membrane surface was tested u...

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Section: Introductionmentioning

confidence: 99%

Enhanced separation and antifouling properties of PVDF ultrafiltration membranes with surface covalent self-assembly of polyethylene glycol

Zhao

Xuan

2015

RSC Adv.

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“…These interactions are widely used in colloidal stabilization (4)(5)(6) and in the last few years have found application on the development of biocompatible materials and drug carriers (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25). The basic idea is that the grafted polymer layer prevents nonspecific adsorption of proteins on the surface of the biocompatible material or drug carrier, reducing the immunological response (7,8,15,(26)(27)(28)(29). The understanding of the kinetics of protein adsorption and its reduction/prevention by grafted polymer layers is therefore very important for the design of materials interacting with biological systems.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of Protein Adsorption and Desorption on Surfaces with Grafted Polymers

Fang

Satulovsky

Szleifer

2005

Biophysical Journal

151

156

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The kinetics of protein adsorption are studied using a generalized diffusion approach which shows that the time-determining step in the adsorption is the crossing of the kinetic barrier presented by the polymers and already adsorbed proteins. The potential of mean-force between the adsorbing protein and the polymer-protein surface changes as a function of time due to the deformation of the polymer layers as the proteins adsorb. Furthermore, the range and strength of the repulsive interaction felt by the approaching proteins increases with grafted polymer molecular weight and surface coverage. The effect of molecular weight on the kinetics is very complex and different than its role on the equilibrium adsorption isotherms. The very large kinetic barriers make the timescale for the adsorption process very long and the computational effort increases with time, thus, an approximate kinetic approach is developed. The kinetic theory is based on the knowledge that the time-determining step is crossing the potential-of-mean-force barrier. Kinetic equations for two states (adsorbed and bulk) are written where the kinetic coefficients are the product of the Boltzmann factor for the free energy of adsorption (desorption) multiplied by a preexponential factor determined from a Kramers-like theory. The predictions from the kinetic approach are in excellent quantitative agreement with the full diffusion equation solutions demonstrating that the two most important physical processes are the crossing of the barrier and the changes in the barrier with time due to the deformation of the polymer layer as the proteins adsorb/desorb. The kinetic coefficients can be calculated a priori allowing for systematic calculations over very long timescales. It is found that, in many cases where the equilibrium adsorption shows a finite value, the kinetics of the process is so slow that the experimental system will show no adsorption. This effect is particularly important at high grafted polymer surface coverage. The construction of guidelines for molecular weight/surface coverage necessary for kinetic prevention of protein adsorption in a desired timescale is shown. The time-dependent desorption is also studied by modeling how adsorbed proteins leave the surface when in contact with a pure water solution. It is found that the kinetics of desorption are very slow and depend in a nonmonotonic way in the polymer chain length. When the polymer layer thickness is shorter than the size of the protein, increasing polymer chain length, at fixed surface coverage, makes the desorption process faster. For polymer layers with thickness larger than the protein size, increases in molecular weight results in a longer time for desorption. This is due to the grafted polymers trapping the adsorbed proteins and slowing down the desorption process. These results offer a possible explanation to some experimental data on adsorption. Limitations and extension of the developed approaches for practical applications are discussed.

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“…When PEG molecules are adsorbed on a surface, a heavily hydrated polymer layer is formed, rendering adsorption of proteins entropically and enthalpically unfavorable [11]. In order to cover different types of surfaces with PEG, the molecule was directly grafted onto the substrate [15,24,25] or coupled to polyelectrolytes, such as poly(l-lysine) (PLL) [26] or poly(acrylic acid) [19], which were subsequently adsorbed as a monolayer.…”

Section: Introductionmentioning

confidence: 99%

Polyelectrolyte multilayer films with pegylated polypeptides as a new type of anti-microbial protection for biomaterials

et al. 2004

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Adhesion of bacteria at the surface of implanted materials is the first step in microbial infection, leading to post-surgical complications. In order to reduce this adhesion, we show that poly(L-lysine)/poly(L-glutamic acid) (PLL/PGA) multilayers ending by several PLL/PGA-g-PEG bilayers can be used, PGA-g-PEG corresponding to PGA grafted by poly(ethylene glycol). Streaming potential and quartz crystal microbalance-dissipation measurements were used to characterize the buildup of these films. The multilayer films terminated by PGA and PGA-g-PEG were found to adsorb an extremely small amount of serum proteins as compared to a bare silica surface but the PGA ending films do not reduce bacterial adhesion. On the other hand, the adhesion of Escherichia coli bacteria is reduced by 72% on films ending by one (PLL/PGA-g-PEG) bilayer and by 92% for films ending by three (PLL/PGA-g-PEG) bilayers compared to bare substrate. Thus, our results show the ability of PGA-g-PEG to be inserted into multilayer films and to drastically reduce both protein adsorption and bacterial adhesion. This kind of anti-adhesive films represents a new and very simple method to coat any type of biomaterials for protection against bacterial adhesion and therefore limiting its pathological consequences.

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Protein Adhesion at Poly(ethylene glycol) Modified Surfaces

Cited by 19 publications

References 0 publications

Enhanced separation and antifouling properties of PVDF ultrafiltration membranes with surface covalent self-assembly of polyethylene glycol

Enhanced separation and antifouling properties of PVDF ultrafiltration membranes with surface covalent self-assembly of polyethylene glycol

Kinetics of Protein Adsorption and Desorption on Surfaces with Grafted Polymers

Polyelectrolyte multilayer films with pegylated polypeptides as a new type of anti-microbial protection for biomaterials

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