Polyelectrolyte multilayer films with pegylated polypeptides as a new type of anti-microbial protection for biomaterials

Abstract

Adhesion of bacteria at the surface of implanted materials is the first step in microbial infection, leading to post-surgical complications. In order to reduce this adhesion, we show that poly(L-lysine)/poly(L-glutamic acid) (PLL/PGA) multilayers ending by several PLL/PGA-g-PEG bilayers can be used, PGA-g-PEG corresponding to PGA grafted by poly(ethylene glycol). Streaming potential and quartz crystal microbalance-dissipation measurements were used to characterize the buildup of these films. The multilayer films terminated by PGA and PGA-g-PEG were found to adsorb an extremely small amount of serum proteins as compared to a bare silica surface but the PGA ending films do not reduce bacterial adhesion. On the other hand, the adhesion of Escherichia coli bacteria is reduced by 72% on films ending by one (PLL/PGA-g-PEG) bilayer and by 92% for films ending by three (PLL/PGA-g-PEG) bilayers compared to bare substrate. Thus, our results show the ability of PGA-g-PEG to be inserted into multilayer films and to drastically reduce both protein adsorption and bacterial adhesion. This kind of anti-adhesive films represents a new and very simple method to coat any type of biomaterials for protection against bacterial adhesion and therefore limiting its pathological consequences.

Multilayer Polyelectrolyte Films Functionalized by Insertion of Defensin: a New Approach to Protection of Implants from Bacterial Colonization

“…3B). The zeta potential alternates between positive and negative values for the PEI-(PSS-PAH) 2 -(PGA-PLL)-PGA film, confirming the buildup of the film. After the deposition of the second PGA layer, defensin was adsorbed and the absolute value of the zeta potential increased by 15 mV; however, the potential remained negative.…”

“…2A). PEI-(PSS-PAH) 2 -(PGA-PLL) 2 -PGA films had a thickness of about 40 nm. The changes in the resonance frequency (⌬f) monitored by the QCM technique (Fig.…”

“…In fact, one of the major advantages of the multilayer technique is precisely that it allows the deposition of multiple layers of the proteins or the peptides tested. The number of peptide layers was increased by embedding the peptide in the following architecture: PEI-(PSS-PAH) 2 -(PGAdefensin-PLL) n , with n equal 1, 2, 5, or 10. In these experiments, the defensin concentration in solution was kept constant at 10 M. As seen in Fig.…”

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

“…It is also applicable to substrates of any shape, it is environmentally-friendly and it allows room temperature processing and low-cost manufacturing [25]. Polyelectrolyte multilayered films have therefore been considered for biomedical application such as capsules for drug delivery [26,27], immunosensing [28], regenerative neurobiology [29], antibacterial and anti fungal protection [30,31]. Despite its potential, LbL has not yet been translated for routine application in the manufacture of medical devices, and further research is required to demonstrate its practical value.…”

Electrochemical Biosensors - Sensor Principles and Architectures

“…Besides their importance in characterization, these techniques also provide unique possibilities for research. It is now possible to manipulate interfaces with electric fields, to study the effect of applied fields onto binding affinities of charged molecules and to control the release of drugs from polyelectrolyte layers or other embedded systems by electronic means [114,291].…”