Protein accumulation in the maize endosperm: role of polyribosomes and the cytoskeleton

Abe, Shunnosuke; Azama, Kishu; Sugimoto, Hideki; Davies, Eric

doi:10.1016/s0981-9428(02)00016-5

Cited by 12 publications

(10 citation statements)

References 38 publications

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“…Several RNA binding activities have been identified in an enriched cytoskeleton PB fraction that may serve as this RNA-anchoring activity (SamiSubbu et al, 2000(SamiSubbu et al, , 2001. Second, actin filaments also have been suggested to serve as a scaffold for polysomes or as sites enriched for translational factors (Lenk et al, 1977;Hesketh, 1994;Abe et al, 2003). If the physical association of translational complexes with actin filaments is necessary for efficient protein synthesis, this requirement would be fulfilled by the cortical region, which contains the highest density of cytoskeletal elements in plant cells (Kost and Chua, 2002).…”

Section: Discussionmentioning

confidence: 99%

Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm

Hamada

Ishiyama

Sakulsingharoj

et al. 2003

THE PLANT CELL ONLINE

108

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Prolamine and glutelin RNAs are localized to two subdomains of the cortical endoplasmic reticulum (ER), the protein body ER and the cisternal ER, in developing rice seeds. The addition of nearly full-length prolamine sequences at the 3 untranslated region of a reporter RNA redirects its localization from the cisternal ER to the protein body ER. Deletion analysis of prolamine RNA sequences indicates the presence of two partially redundant cis elements required for protein body ER targeting. The addition of glutelin 3 untranslated region to protein body ER cis sequences, however, redirects RNA localization to the cisternal ER. These results indicate that there are at least two regulated RNA transport pathways as well as a constitutive pathway to the cortical ER.

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Section: Discussionmentioning

confidence: 99%

Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm

Hamada

Ishiyama

Sakulsingharoj

et al. 2003

THE PLANT CELL ONLINE

108

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“…This results in an endosperm with less zein (which is low in lysine) and the production of more high-lysine proteins, thus enhancing the nutritional value of maize. Stankovic´et al (2000) showed that actin was increased in the opaque-2 mutant and also associated with protein body fractions when separated on sucrose gradients, while Abe et al (2003) showed that polyribosomes from opaque-2 were more efficient in translation. These observations indicate that zein deposition in protein bodies is closely related to actin and translational activity represented by the elongation factor, eEF1a (Clore et al 1996;Davies et al 1998Davies et al , 2001.…”

Section: Introductionmentioning

confidence: 99%

Lysine-containing proteins in maize endosperm: a major contribution from cytoskeleton-associated carbohydrate-metabolizing enzymes

Azama

Abe

Sugimoto

et al. 2003

Planta

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We measured fresh weight, dry weight, total protein, and the amounts of several individual proteins during endosperm development in three varieties of maize ( Zea mays L.): W64A wild-type (WT) and opaque-2 (o2), and sweet corn (SW). By 28 days after pollination (DAP), fresh weight was much higher in WT and SW than in o2, but o2 had a higher dry weight and thus a much lower water content. By 28 DAP, protein concentration [mg (g tissue(-1))] was highest in o2 and lowest in WT, while the protein content (microg seed(-1)) was lowest in o2. The storage proteins, alpha- and gamma-zeins, were low initially, but by 28 DAP they comprised over 50% of the total protein in WT and SW, but only about 30% in o2. In all varieties, the cytoskeleton proteins, actin, tubulin and eEF1alpha, sedimented with the protein bodies at 30 g to 27,000 g in tissue homogenized in cytoskeleton-stabilizing buffer. Other cytoskeleton-associated proteins increased during development, including UDP-glucose starch glucosyltransferase (UDP-GSGT, EC 2.4.1.11), sucrose synthase 1 (SuSy-1, EC 2.4.1.13) and fructose-1,6 bisphosphate aldolase (FBA, EC 4.1.2.13). At 28 DAP, these cytoskeleton-associated proteins combined make up 27% (WT), 23% (SW) and 33% (o2) of the total protein. These proteins are all rather high (5-11%) in lysine, and so they contribute about 75% (WT), 67% (o2), and 51% (SW) of the total endosperm lysine. We conclude that efforts to elevate the levels of these proteins could make a significant contribution to the nutritional value of corn.

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“…Changes in the profiles of polysomes, which contain mRNAs undergoing translation, enable us to follow very precisely the rate of in vivo synthesis of proteins in cells [40].…”

Section: Discussionmentioning

confidence: 99%

Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.)

Brosowska-Arendt

Weidner

2012

Acta Soc Bot Pol

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IntroductionChanges in the environment, such as drought, salinity, high or low temperature, are an important factor which affects the growth of crops and the volume of crop yields [1][2][3]. Very important legume grown and consumed extensively worldwide is pea [4]. As a rich source of proteins, carbohydrates, fibre, vitamins and minerals, peas are important in human nutrition [5]. Pea is the fourth leading legume in terms of consumption in the world after soybean, peanuts and bean [6]. One of the major abiotic stress often occurs in Europe is osmotic stresses induced with polyethylene glycol (within −0.5 MPa). The earliest metabolic change caused by water stress is a decreased amount of polysomes [7]. Once the polysomes have been disaggregated, the plant growth is inhibited due to a slower protein synthesis rate [7][8][9]. Considerable reduction in the amount of polysomes in plant tissues is observable after osmotic stress lasting for just 20-30 minutes [10]. Decrease of the content of polysomes in response to abiotic stresses is connected with the process of "switching" the expression of genes from those participating in the growth and development of plants under unstressed conditions to the ones active in response to stress [11].In plant tissues, polysomes can occur as free polysomes (FP), endoplasmic reticulum membrane-bound polysomes (MBP) [12], cytoskeleton-bound polysomes (CBP) [13,14] and cytoskeleton-membrane-bound polysomes (CMBP) [15]. Changes in the distribution of polysomes between the particular fractions reflect changes in the complement of proteins, as each population of polysomes is engaged in the synthesis of specific proteins [16]. Thus, the FP population is mainly involved in the synthesis of soluble proteins of the cytoplasm, cellular nucleus, mitochondria and peroxisomes [17]; the MBP population is engaged in the production of secretory proteins, lysosome proteins and the proteins which are an integral component of the plasmatic membrane and intracellular membranes, including the endoplasmic reticulum [16,17]; finally, the CBP and CMBP populations are responsible for the synthesis of cytoskeleton and stress proteins [16,[18][19][20][21]. The largest share in the total polysome pool consists of the CBP population, which sometimes reaches 70% of the total polysome content [22]. Polysomes contain mRNAs, which may undergo selective translation, result in modification of protein synthesis in response to stress conditions in plants [23,24].The objective of this study was to indicate differences in the composition of polysome-bound proteins and in the products of in vitro translation in pea (Pisum sativum L.) seeds germinating under unstressed conditions and under long-and short-term osmotic stress (−0.5 MPa) followed by post-stress recovery. AbstractPlant growth throughout the world is often limited by unfavourable environmental conditions. This paper reports results of a study on long-and short-term osmotic stress (−0.5 MPa) followed by a recovery on in vitro translational capacity of polysomes a...

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Protein accumulation in the maize endosperm: role of polyribosomes and the cytoskeleton

Cited by 12 publications

References 38 publications

Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm

Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm

Lysine-containing proteins in maize endosperm: a major contribution from cytoskeleton-associated carbohydrate-metabolizing enzymes

Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.)

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