Protective Roles of N-trans-feruloyltyramine Against Scopolamine-Induced Cholinergic Dysfunction on Cortex and Hippocampus of Rat Brains

Abstract

Objective: To study the protective effects of N-trans-feruloyltyramine (NTF) on scopolamine-induced cholinergic dysfunction, apoptosis, and inflammation in rat brains. Materials and Methods: Treatments were administered intraperitoneally (i.p.). Wistar rats (8-week-old) were allocated into 4 groups (n = 3) as follows: scopolamine-only, NTF-only, NTF + scopolamine and control. Spatial cognition was evaluated by Morris water maze. ROS assay and Western blot analyses were conducted in 3 brain regions: the frontal cortex, hippocampus, and temporal cortex. Results: NTF treatment inhibited scopolamine-induced memory impairment and significantly attenuated scopolamine-induced changes in the three brain regions. Investigated scopolamine-associated changes were as follows: increases in ROS production and BACE1 level, decrease in ChAT level, increases in inflammatory and apoptotic markers, and activation of signaling pathway kinases related to inflammation and apoptosis. Conclusion: With its in vivo antioxidant, cholinergic-promoting, anti-apoptosis, and anti-inflammatory biological activities, NTF is a promising candidate to be further investigated as a potential treatment for Alzheimer’s-associated neurodegeneration.

Metabolite Profiling of Allium hookeri Leaves Using UHPLC-qTOF-MS/MS and the Senomorphic Activity of Phenolamides

“…Consequently, the inhibition of SASP markers could potentially contribute to wound healing, aligning with the traditional use of A. hookeri. Furthermore, this is the first report of the senomorphic activity of N-trans-feruloyltyramine, which has previously been demonstrated to possess a wide range of biological effects, such as protection against scopolamine-induced cholinergic dysfunction on the cortex and hippocampus [45] and β-amyloid peptide-induced neurotoxicity [46], the inhibition of melanin biosynthesis [47], antioxidant, cytotoxic activities, and protection against H 2 O 2 -induced oxidative damage [48].…”