Protective role of puerarin on lead-induced alterations of the hepatic glutathione antioxidant system and hyperlipidemia in rats

Liu, Chan-Min; Ma, Jie-Qiong; Sun, Yangying

doi:10.1016/j.fct.2011.09.007

Cited by 102 publications

(77 citation statements)

References 73 publications

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“…[3435] In addition, flavonoids, via suppression of LDL oxidation, may also induce endothelium-dependent vasorelaxation and may increase reverse cholesterol transport. [34] The reason why flavonoids inhibit LDL oxidation could be their chelation of copper ions, as has been demonstrated in the case of quercetin.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of LDL oxidation and oxidized LDL-induced foam cell formation in RAW 264.7 cells show anti-atherogenic properties of a foliar methanol extract of Scoparia dulcis

et al. 2014

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Background:Oxidation of low density lipoproteins and their further uptake by macrophages is known to result in the formation of foam cells, which are critical in the initiation of atherosclerosis through activation of inflammatory signalling cascades. Thus, powerful dietary antioxidants are receiving attention for the reversal of such pathological states.Materials and Methods:Extracts of Scoparia dulcis have been used as tea and health drinks with various health promoting effects. In the present study, we examined the reactive oxygen scavenging potential as well as anti-inflammatory and anti-atherogenic efficacies, using leaf extracts obtained after successive extraction with various solvents.Results:A methanol extract showed potent antioxidant activity with an IC50 value of 570 μg/ml, caused hydrogen peroxide scavenging (28.9 µg/ml) and anti-inflammatory effects by improving human erythrocyte membrane stabilisation (about 86%). The methanol extract also efficiently inhibited lipid peroxidation and oxidation of low density lipoproteins, thus preventing foam cell formation in cultured RAW 264.7 cells. Furthermore, phytochemical screening of the extracts showed high accumulation of flavonoids.Conclusions:The foliar methanol extract of Scoparia dulcis has a strong anti-atherogenic potential and this property could be attributed maybe due to presence of flavonoids since HPLC analysis showed high concentrations of myricetin and rutin in the methanol extract.

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Section: Discussionmentioning

confidence: 99%

Inhibition of LDL oxidation and oxidized LDL-induced foam cell formation in RAW 264.7 cells show anti-atherogenic properties of a foliar methanol extract of Scoparia dulcis

et al. 2014

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“…It is found that puerarin, a flavonoid derivative, reduces the growth of radicals and MDA levels in liver tissues of rats administered lead. 20 Moreover, it is observed that lipid peroxidation products and oxidative stress increased by a considerable amount in the brain tissues of rats administered 500 ppm lead. 12 In addition, researchers have frequently reported the effects of heavy metal ions on different enzymes in previous years and inhibition effect of heavy metals on metabolically imported enzyme such as CA 21 , glucose 6-phopsphate dehydrogenase 22 , cytochrome P450 reductase 23 , glutathione S-transferase 24 have been determined.…”

Section: Discussionmentioning

confidence: 99%

<i>In Vivo</i> Effects of Naringenin and Lead on Rat Erythrocyte Carbonic Anhydrase Enzyme

Kuzu¹,

Özkaya²,

Şahin³

et al. 2017

tjps

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ÖZAmaç: Karbonik anhidraz (CA) enzimi CO 2 'nin su ile tersinir reaksiyonunu katalizler ve sistemik asit baz dengesi, solunum gibi metabolik açıdan oldukça önemli olaylarda görev alır. Bu çalışmada canlıların farklı yollarla maruz kaldığı bir ağır metal olan kurşun ile bir flavanon olan naringeninin enzim üzerine in vivo etkisi araştırıldı. Gereç ve Yöntemler: Bu amaçla, dört farklı sıçan grubu oluşturuldu ve bunlardan biri kontrol grubu olarak belirlendi. Diğer üç gruba ise kurşun, naringenin ve kurşun+naringenin maddeleri verildi ve eritrositlerdeki CA enzim aktivitesindeki değişimler incelendi. Bulgular: Araştırma bulguları kontrol grubundaki enzim aktivitesinin diğer gruplardan daha fazla olduğunu gösterdi. Kurşun grubu en düşük inhibisyon etkisini gösterirken, narigenin grubu en yüksek inhibisyon etkisini gösterdi. Sonuç: Bu nedenle naringeninin CA enzimi için kuvvetli bir inhibitör olduğu söylenebilir. Anahtar kelimeler: Karbonik anhidraz, inhibisyon, kurşun, naringenin Objectives: Carbonic anhydrase (CA) enzyme catalyses the reversible reactions of CO 2 with water and takes part in metabolically important events such as systemic acid-base regulation and respiration. In this study, in vivo effects of lead, which is a heavy metal and to which living beings are exposed by different ways, with naringenin, a flavanone, were investigated. Materials and Methods: For this purpose, four different rat groups were established and one of them was chosen as the control group. The other three groups were given lead, naringenin and lead+naringenin substances to analyze the changes in the CA enzyme of rat erythrocytes. Results:The research findings showed that the enzyme activity in the control group was higher than that in the other groups. The naringenin group showed the highest inhibition effect, while the lead group showed the lowest inhibition. Conclusion:Therefore, it can be said that naringenin is a strong inhibitor of the CA enzyme.

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“…Well documents are available about this pollutant causes neurological deterioration like is brain injury, mental breakdown and many behavioural problems (Radad 2014). Numerous studies showed that phenolic compounds could decrease tissues loading of heavy metal (Xia et al 2010, Liu et al 2011, Aksu et al 2012, Radad et al 2014. In this study, the neuroprotective potential of polyphenolics enriched PJ on the brain damage and antioxidant system were investigated in rats against low level Pb exposure.…”

Section: Discussionmentioning

confidence: 99%

The investigation of neuroprotective effects of pomegranate juice on oxidative damage in brain caused by lead in rats

Aksu¹,

Sağlam²,

Aksu³

2016

EurasianJVetSci

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ÖzAmaç: Bu çalışmada, düşük düzeyde kurşun (Pb) maruziyetinin sıçanlarda beyin dokusu ve antioksidan sistem üzerin-de oluşturduğu hasara karşı nar suyunun (NS) nöroprotektif potansiyelinin araştırılması amaçlandı. Gereç ve Yöntem:Araştırmada kullanılan toplam 40 adet rat, her birinde 10'ar rat bulunan 4 gruba ayrıldı. Kontrol grubu (1), standart rat yemi ve içme suyuyla beslendi. Pozitif kontrol grubuna içme suyuyla günlük 2000 ppm kurşun (kurşun asetat); düşük tedavi grubuna (3), içme suyuyla günlük 2000 ppm kurşun ve gavajla 30 µL NS; yüksek tedavi grubuna (4) ise içme suyuyla günlük 2000 ppm kurşun ve gavajla 60 µL NS verildi. Araştırma 5 hafta sürdürüldü. Sıçan beyinlerinde malondialdehide (MDA) ve glutatyon (GSH) seviyeleri ile süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri belirlendi. Ayrıca beyin dokusunda histopatolojik incelemeler yapıldı. Bulgular: Nar suyu kurşunun sebep olduğu lipid peroksidasyonu azaltarak (düşük MDA düzeyi) antioksidan enzim (SOD ve CAT) aktivitelerini ve GSH düzeyini artırarak oksidatif stresi hafifletti. Sadece kurşun alan grupta şiddetli nörode-jeneratif değişiklikler görüldü. Nar suyunun, kurşunun beyin dokusunda oluşturduğu hücresel hasarı kısmen önlediği belirlendi.Öneri: Düzenli nar suyu tüketimi artan yoğun sanayileşme-den kaynaklanan kronik kurşun maruziyetine karşı faydalı olabilir.Anahtar kelimeler: Kurşun, nar suyu, fenolik bileşenler, oksidatif stres, antioksidan Abstract Aim: The aim of the study was to investigate the neuroprotective potential of pomegranate juice (PJ) against the damage of brain tissue and antioxidant system induced low level lead (Pb) exposure in rats. Materials and Methods:A total of 40 rats were divided into four groups containing 10 rats in each. The control group (1) was fed standart rat feed and daily water. A positive control group (2) received a daily dose of 2000 ppm lead (lead acetate) in drinking water; a low treatment group (3) that received a daily dose of 2000 ppm lead together with 30 µL PJ by oral gavage; and a high treatment group (4) that received 2000 ppm lead and 60 µL PJ by oral gavage daily. The experiment was lasted for 5 weeks. Levels of malondialdehyde (MDA) and glutathione (GSH) were determined as well as the activities of superoxide dismutase (SOD) and catalase (CAT). Morever, histopathological examination was also performed in the brain of the rats.Results: Pomagranete juice alleviated oxidative stress by decreasing lipid peroxidation ( low MDA level) and increasing the activities of antioxidant enzymes (SOD and CAT) and GSH level in the rats exposed to lead. Severe neurodejenerative changes were observed in only groups received lead. Cellular damage of the brain was partially prevented by PJ. Conclusion:Regular consumption of pomegranate juice may provide significant benefits against the threat of chronic heavy metal exposure due to increasing intensive industrialization.Keywords: Lead, pomegranate juice, phenolic compounds, oxidative stress, antioxidant. RESEARCH ARTICLE

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Protective role of puerarin on lead-induced alterations of the hepatic glutathione antioxidant system and hyperlipidemia in rats

Cited by 102 publications

References 73 publications

Inhibition of LDL oxidation and oxidized LDL-induced foam cell formation in RAW 264.7 cells show anti-atherogenic properties of a foliar methanol extract of Scoparia dulcis

Inhibition of LDL oxidation and oxidized LDL-induced foam cell formation in RAW 264.7 cells show anti-atherogenic properties of a foliar methanol extract of Scoparia dulcis

<i>In Vivo</i> Effects of Naringenin and Lead on Rat Erythrocyte Carbonic Anhydrase Enzyme

The investigation of neuroprotective effects of pomegranate juice on oxidative damage in brain caused by lead in rats

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