&lt;i&gt;In Vivo&lt;/i&gt; Effects of Naringenin and Lead on Rat Erythrocyte Carbonic Anhydrase Enzyme

Kuzu, Müslüm; Özkaya, Ahmet Kağan; Şahin, Zafer; Dağ, Üzeyir; Çomaklı, Veysel; Demirdağ, Ramazan

doi:10.4274/tjps.13008

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…It was reported that CA activity in rat erythrocytes was significantly decreased exposed to naringenin, a natural flavone. In addition, it is known that the extracts containing polyphenols and flavones obtained from various plants and fungal species inhibit the activities of CA I and II (9). Inhibitory effects of the different extracts from Alcea dissecta on hCA I and II were determined for the first time in this study.…”

Section: Esterase Activitymentioning

confidence: 90%

“…Carbonic anhydrases (CAs, EC 4.2.1.1) as ubiquitous widespread metalloenzymes catalyze the crucial reaction for all living organisms: the hydration process of CO 2 to HCO 3 -and H + (8,9). Fifteen isoenzymes of CA encoded from the α-CA gene family have been identified and characterized for human beings as cytosolic forms (hCA I, II, III, VII, and XIII), membrane-bound forms (hCA IV, IX, XII, and XIV), mitochondrial forms (hCA VA and VB).…”

Section: Introductionmentioning

confidence: 99%

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In vitro Biological Activities of Different Extracts from Alcea dissecta

Taşkın

KAHVECİOĞLU

Türkoğlu

et al. 2022

Clinical and Experimental Health Sciences

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Objective: Alcea genus belongs to Malvaceae family and this genus is represented by 85 taxa in the world and 21 taxa in the Flora of Turkey. The flowers of Alcea genus contain plenty of mucilage and are used for the treatment of respiratory diseases such as asthma and cough. Alcea dissecta is known as 'Govik, Hiro, Hero' in Turkey, and the flowers and leaves of this species have been used in the treatment of asthma, injury, colds, and gastrointestinal diseases in Turkey. To the best of our knowledge, there is no report on the effect of extraction methods on the biological activity of this plant. In addition, although this species is being used as a medical plant, there is no study of the antioxidant, antiurease, esterase, and anticholinesterase activity of the plant. Therefore, the aim of this study was to evaluate in vitro antioxidant, anti-urease, esterase, anticholinesterase activities of Alcea dissecta using a variety of extracts. Methods:The antioxidant activities of different extracts were examined by DPPH, ABTS, FRAP, and CUPRAC methods. The total phenolic compounds contained in the extracts were determined using the Folin-Ciocalteu reagent (FCR) method. Anti-urease and anticholinesterase activities of different extracts were evaluated by indophenol and Ellman methods respectively. In addition, esterase activities of plant extracts were determined. Results:In the present study, ethanol:water (1:1, v/v) and chloroform extracts obtained maceration method showed stronger DPPH and ABTS radical scavenging activity than other extracts. The chloroform extract obtained Soxhlet method was found to have higher FRAP and CUPRAC values than other extracts. It was also found that the ethanol extract obtained maceration method showed the most potent anti-urease and anticholinesterase activity. According to the results, the strongest inhibitory effect on both hCA I and II isoenzymes was shown by the petroleum ether extract obtained Soxhlet method. Conclusion:As a result, it was determined that different plant extracts have antioxidant, anti-urease, esterase, anticholinesterase activities. In addition, the data obtained from this study will shed light on future research on the biological activities of this species.

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Section: Esterase Activitymentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

In vitro Biological Activities of Different Extracts from Alcea dissecta

Taşkın

KAHVECİOĞLU

Türkoğlu

et al. 2022

Clinical and Experimental Health Sciences

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“…21,22 Carbonic anhydrase (CA, EC 4.2.1.1) is a metalloenzyme including Zn 2+ in the active region, found in all living species, which catalyzes the reversible conversion of carbon dioxide to bicarbonate and H + . 23 This reaction plays a critical role in many physiological processes associated with metabolic pathways, including carbon dioxide. 24 Therefore, the regulation of enzyme activity has emerged as an approach in the treatment of diseases such as neuropathic pain, 25 glaucoma, edema, idiopathic intracranial hypertension, 26 cerebral ischemia, 27 cancer, 28 and epilepsy.…”

Section: Introductionmentioning

confidence: 99%

Microvawe assisted synthesis of N-(methyl and methoxy) benzylidene-4-fluoroaniline derivatives and their carbonic anhydrase I and II inhibition properties

Çelik¹,

Kuzu²

2019

Org.Commun.

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In this study, some Schiff base derivatives (6a-f) were synthesized using a microwave method. The synthesized compounds were characterized by 1D, 2D NMR and mass spectral data. Their inhibitory effects were studied on carbonic anhydrase isozymes (hCA I and II), purified from human erythrocyte cells by Sepharose-4B-Ltyrosine-sulfanilamide affinity chromatography and the compounds N-3-Methylbenzylidene-4-fluoroaniline (6b), N-4-Methylbenzylidene-4-fluoroaniline (6c), N-2-Methoxybenzylidene-4-fluoroaniline (6d)showed moderate activity on hCAII in the range of IC50 values.

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“…In this condition, fluid secretion into the cysts and a malfunction in proliferation cause kidney cysts to form. 23 About 85% of instances of ADPKD are caused by "TRPP1 (polycystic kidney disease-1)" gene and protein mutations, while 15% are due to TRPP2 (PKD2) protein and gene mutations. 24 We thus investigated the role of TRPP2 in naringenin's As a follow-up to our first findings in Dictyostelium, we examined the influence effects on cyst development and "Madin-Darby canine kidney (MDCK)" cell proliferation.…”

mentioning

confidence: 99%

In-vivo Evaluation of Nephroprotective Activity of Naringenin against ADPKD and MDCK-derived Cysts

Varalakshmi,

Chitra

2023

IJPQA

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Aim: Determining the in-vivo assessment of Naringenin’s nephroprotective efficacy against Autosomal dominant polycystic kidney disease (ADPKD) and Madin-Darby canine kidney (MDCK) derived cysts. Method: Our research on the molecular mechanism of naringenin, a flavonoid present in plants and berries that has been shown to limit cell growth and protect against cancer in in-vitro and animal models, was conducted using dictyostelium, a simple, controllable biomedical model. Cultured MDCK cells were used to generate differentiated tubule cells, and these findings were extrapolated to a human kidney model employing these cells. Results: While naringenin inhibited growth in dictyostelium, it had no effect on development. In a random-gene-knockout screen, a TRPP2 (polycystin-2) knockout mutant was discovered to be resistant to naringenin’s effects on growth and random-cell movement. Changes in the divalent transient receptor cause polycystic kidney disease type 2 potential cation channel TRPP2. We found that the growth of cysts and MDCK cells might be inhibited by naringenin. Partial resistance to naringenin was achieved in this model by lowering TRPP2 levels via siRNA, as evidenced by the presence of larger cysts following treatment with 3 and 10 M naringenin compared to controls. Naringenin had no effect on chloride secretion. Conclusion: Naringenin’s influence on cell proliferation is mediated by TRPP2 in both dictyostelium and mammalian kidney cells, despite their vast evolutionary distance from one another (polycystin-2). Naringenin will be the subject of more research as a possible new therapeutic treatment for ADPKD

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<i>In Vivo</i> Effects of Naringenin and Lead on Rat Erythrocyte Carbonic Anhydrase Enzyme

Cited by 5 publications

References 27 publications

In vitro Biological Activities of Different Extracts from Alcea dissecta

In vitro Biological Activities of Different Extracts from Alcea dissecta

Microvawe assisted synthesis of N-(methyl and methoxy) benzylidene-4-fluoroaniline derivatives and their carbonic anhydrase I and II inhibition properties

In-vivo Evaluation of Nephroprotective Activity of Naringenin against ADPKD and MDCK-derived Cysts

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