Protective role of mucilage against Al toxicity to root apex of pea (Pisum sativum)

Geng, Meiyu; Xu, Mengxia; Xiao, Hongdong; Wang, Huizhen; He, Li; Zhao, Zhong-Qiu; Yu, Min

doi:10.1007/s11738-011-0919-1

Cited by 9 publications

(4 citation statements)

References 33 publications

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“…These findings are in line with previous works that show the capacity of P. harmala to thrive in the presence of a wide range of metals while developing a resilient and deeply rooted system [ 60 ]. This adaptability might be attributed to its ability to release high- and low-molecular-weight root exudates, which shield the root apex from metal toxicity during severe environmental stresses [ 61 , 62 , 63 ]. These exudates, coupled with modifications in cell wall and plasma membrane properties, provide mechanical strength to the root system, further enhancing its ability to withstand harsh abiotic stressors.…”

Section: Discussionmentioning

confidence: 99%

Enhancing Native Plant Establishment in Mine Tailings under Drought Stress Conditions through the Application of Organo-Mineral Amendments and Microbial Inoculants

Atika,

Leila,

Pereira

et al. 2024

Plants

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The implementation of phytoremediation strategies under arid and semiarid climates requires the use of appropriate plant species capable of withstanding multiple abiotic stresses. In this study, we assessed the combined effects of organo-mineral amendments and microbial inoculants on the chemical and biological properties of mine tailings, as well as on the growth of native plant species under drought stress conditions. Plants were cultivated in pots containing 1 kg of a mixture of mine tailings and topsoil (i.e., pre-mined superficial soil) in a 60:40 ratio, 6% marble sludge, and 10% sheep manure. Moreover, a consortium of four drought-resistant plant growth-promoting rhizobacteria (PGPR) was inoculated. Three irrigation levels were applied: well-watered, moderate water deficit, and severe water deficit, corresponding to 80%, 45%, and 30% of field capacity, respectively. The addition of topsoil and organo-mineral amendments to mine tailings significantly improved their chemical and biological properties, which were further enhanced by bacterial inoculation and plants’ establishment. Water stress negatively impacted enzymatic activities in amended tailings, resulting in a significant decrease in acid and alkaline phosphatases, urease, and dehydrogenase activities. Similar results were obtained for bacteria, fungi, and actinomycete abundance. PGPR inoculation positively influenced the availability of phosphorus, total nitrogen, and organic carbon, while it increased alkaline phosphatase, urease (by about 10%), and dehydrogenase activity (by 50%). The rhizosphere of Peganum harmala showed the highest enzymatic activity and number of culturable microorganisms, especially in inoculated treatments. Severe water deficit negatively affected plant growth, leading to a 40% reduction in the shoot biomass of both Atriplex halimus and Pennisetum setaceum compared to well-watered plants. P. harmala showed greater tolerance to water stress, evidenced by lower decreases observed in root and shoot length and dry weight compared to well-watered plants. The use of bioinoculants mitigated the negative effects of drought on P. harmala shoot biomass, resulting in an increase of up to 75% in the aerial biomass in plants exposed to severe water deficit. In conclusion, the results suggest that the combination of organo-mineral amendments, PGPR inoculation, and P. harmala represents a promising approach to enhance the phytoremediation of metal-polluted soils under semiarid conditions.

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Section: Discussionmentioning

confidence: 99%

Enhancing Native Plant Establishment in Mine Tailings under Drought Stress Conditions through the Application of Organo-Mineral Amendments and Microbial Inoculants

Atika,

Leila,

Pereira

et al. 2024

Plants

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“…Last but not the least, previous studies have shown that the RBCs and their mucilage could form a protective sheath at the root tip, which prevented Al from diffusing into the root tip, thereby alleviating Al stress in plant root tip (7,28,45,46). The mucilage consists of a variety of molecules including cell wall polysaccharides and proteoglycans, secondary metabolites, anti-microbial proteins and peptides, and extracellular DNA (13,(47)(48)(49)(50).…”

Section: The Ideal Plant Single Cell System Of Rbcsmentioning

confidence: 99%

“…The mucilage consists of a variety of molecules including cell wall polysaccharides and proteoglycans, secondary metabolites, anti-microbial proteins and peptides, and extracellular DNA (13,(47)(48)(49)(50). Mucilage can strongly bind Al in the rhizosphere (46). The mucilage can protect root tips from Al toxicity by tightly binding Al and sequestrating Al in mucilage, which consequently reduced the inhibition of root growth induced by Al and the absorption of Al by roots (51).…”

Section: The Ideal Plant Single Cell System Of Rbcsmentioning

confidence: 99%

Root Border Cells as a Convenient Single Cell System to Study Plant-Environmental Interactions: A Case Study for Aluminum Tolerance

Ying

Chen

et al. 2022

Front. Soil Sci.

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Root border cells (RBCs) are a group of cells that originated from the root cap meristem, which are developed by genetic regulation and play a variety of biological functions. Being composed of a homologous single cell population with high metabolic activity and intact cell walls, RBCs represent a highly useful tool for studying various aspects of plant mineral absorption and utilization, as well as plant-soil-microbiome interactions in the rhizosphere. Research on RBCs also promise to become a hotspot in the context of understanding root adaptive responses to hostile environments. In order to take advantage of RBCs as an ideal single cell system in plant-environmental interactions, we summarized the production and function of RBCs and built-up the methodology for RBCs culturing, purification, and quantity control for plant research. The latter is done by using a case study of the application of RBCs to study mechanisms of Al toxicity in plants. This work offers plant scientists a new cognition of adopting RBCs as a convenient single cell system for the multidisciplinary research including (but not limited to) plant physiology, development and genetics, nutrition, and stress and adaptation. Root border cells (RBCs) are derived from the root cap and represent a population of living cells with special physiological activity and biological roles that are different from the root cap cells per se. After being separated from the root cap, RBCs become more active in metabolism than the progenitor root cap cells; for example, they incorporate labeled amino acids into protein 2.6-fold more efficiently than the cells of the root cap. In addition, mRNA and protein were differentially expressed between root cap cells and RBCs. Since the production of RBCs is genetically regulated and RBCs played a variety of biological functions in resistance to biotic and abiotic stresses occurred in the rhizosphere, RBCs were suggested as an ideal single cell system for the study the response of plant root cells to nutrient availability, environmental stresses, and in plant-microbial interactions. Some studies revealed that RBCs, which development is regulated by endogenous and exogenous signals, are biologically viable in the majority of higher plant species. This work reviews the research on RBCs in plant environment interaction and describes the case study of RBCs as a convenient single cell system to study plant responses to Al toxicity.

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“…Root border cells, living cells surrounding root apices of most plant species ( Hawes et al, 2000 ; Yu et al, 2009 ), are considered to perform pivotal roles in protecting root apices from Al toxicity ( Miyasaka and Hawes, 2001 ; Tamás et al, 2005 ; Xing et al, 2008 ; Yu et al, 2009 ; Geng et al, 2011 ; Shu et al, 2011 ; Cai et al, 2012 , 2013 ). Significantly more Al was found in RBCs than in root apices when RBCs were kept intact under mist culture ( Yu et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Alkali-Soluble Pectin Is the Primary Target of Aluminum Immobilization in Root Border Cells of Pea (Pisum sativum)

Yang

Fang

et al. 2016

Front. Plant Sci.

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We investigated the hypothesis that a discrepancy of Al binding in cell wall constituents determines Al mobility in root border cells (RBCs) of pea (Pisum sativum), which provides protection for RBCs and root apices under Al toxicity. Plants of pea (P. sativum L. ‘Zhongwan no. 6’) were subjected to Al treatments under mist culture. The concentration of Al in RBCs was much higher than that in the root apex. The Al content in RBCs surrounding one root apex (104 RBCs) was approximately 24.5% of the total Al in the root apex (0–2.5 mm), indicating a shielding role of RBCs for the root apex under Al toxicity. Cell wall analysis showed that Al accumulated predominantly in alkali-soluble pectin (pectin 2) of RBCs. This could be attributed to a significant increase of uronic acids under Al toxicity, higher capacity of Al adsorption in pectin 2 [5.3-fold higher than that of chelate-soluble pectin (pectin 1)], and lower ratio of Al desorption from pectin 2 (8.5%) compared with pectin 1 (68.5%). These results indicate that pectin 2 is the primary target of Al immobilization in RBCs of pea, which impairs Al access to the intracellular space of RBCs and mobility to root apices, and therefore protects root apices and RBCs from Al toxicity.

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Protective role of mucilage against Al toxicity to root apex of pea (Pisum sativum)

Cited by 9 publications

References 33 publications

Enhancing Native Plant Establishment in Mine Tailings under Drought Stress Conditions through the Application of Organo-Mineral Amendments and Microbial Inoculants

Enhancing Native Plant Establishment in Mine Tailings under Drought Stress Conditions through the Application of Organo-Mineral Amendments and Microbial Inoculants

Root Border Cells as a Convenient Single Cell System to Study Plant-Environmental Interactions: A Case Study for Aluminum Tolerance

Alkali-Soluble Pectin Is the Primary Target of Aluminum Immobilization in Root Border Cells of Pea (Pisum sativum)

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