Protective mechanism in UV-B treated Crotalaria juncea L. seedlings

Balakrishnan, V.; Venkatesan, K.; Ravindran, K. C.; Kulandaivelu, G.

doi:10.17221/2727-pps

Cited by 17 publications

(5 citation statements)

References 25 publications

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“…The peroxidase enzymes are reported to utilise phenolic compounds as their co-substrate and this fact was further confirmed in the present work by the presence of high phenolic content in the sprouts treated with UV 365 nm. Elevation in the catalase and peroxidase activity has also been observed by other workers in different plant samples under UV irradiation [40]. It can be suggested that the excess H2O2 production might have resulted in induced catalase as well as peroxidase activity in response to UV exposure.…”

Section: Discussionsupporting

confidence: 65%

Elicitation of Therapeutic Potential and Oxidative Stress Assessment of Fenugreek Sprouts Under Uv Irradiation

Gupta

Islam

Choudhuri

et al. 2017

Int J Pharm Pharm Sci

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Objective: To evaluate the effect of ultraviolet (UV) radiation on the therapeutical and oxidative stress status of fenugreek seedlings during the post-germination phase. Methods:The seeds of Trigonella foenum-graecum L. were subjected to UV radiation of different wavelengths UV 254 nm, UV 365 nm and the combination of 254 nm and 365 nm UV during the course of germination up to 96 h. Free radicals scavenging activity, in vitro antidiabetic activity as well as total phenol (TPC) and flavonoid (TFC) contents were evaluated up to four days of germination stages. Analysis of total activities of superoxide dismutase (SOD), peroxidase, catalase and NADPH oxidase (NOX) was conducted using non-denaturing polyacrylamide gel electrophoresis along with histochemical detection of H2O2 and superoxide radical localisation, lipid peroxidation and plasma membrane integrity was also performed. Results:Overall it was observed that the sprouts exposed to UV 365 nm exhibited a significant enhancement in free radical scavenging as well as antidiabetic activity at the 48h stage of germination. Interestingly highest phenol and flavonol content were also stimulated at the same stage by UV 365 nm. The effect of UV irradiation was evident on the localisation of H2O2 and superoxide radical. The on gel assay revealed that the total activities of the antioxidant enzymes were extensively elevated by UV irradiation. Conclusion:The results suggest that the UV irradiation technology can be implemented for the enhancement of nutraceutical properties of sprouts also UV exposure helps in the induction of the antioxidant enzyme system which may be beneficial for oxidative stress management in plants during germination phases.

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Section: Discussionsupporting

confidence: 65%

Elicitation of Therapeutic Potential and Oxidative Stress Assessment of Fenugreek Sprouts Under Uv Irradiation

Gupta

Islam

Choudhuri

et al. 2017

Int J Pharm Pharm Sci

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“…Polyphenoloxidase is also responsible for the oxidation of phenolic compound as reported by Jansen et al (2001). Several studies have shown increased polyphenoloxidase due to UV-B irradiation (Balakrishnan et al, 2005;Santos et al, 1999). Enhanced UV-B radiation activates antioxidant enzymes and induces polyamines and causes leaf damage exemplified by an increase in polyphenoloxidase activity and a decrease in chlorophyll concentration.…”

Section: Antioxidant Enzymesmentioning

confidence: 91%

Effect of ultraviolet-B radiation on biochemical and antioxidant defence system in <i>Indigofera tinctoria</i> L. seedlings

Ravindran¹,

Indrajith²,

Pratheesh³

et al. 2010

Int. J. Eng. Sci. Tech

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The stratospheric ozone depletion and enhanced solar ultraviolet-B (UV-B) irradiance may have adverse impact on living organisms. The impact of UV-B radiation (UV-B, 280~320nm) on growth, biochemical and antioxidant enzyme activity was studied in Indigofera tinctoria (L.) seedling, commonly used as a green manure. The supplementary UV-B radiation significantly decreased the growth, development and changes in UV-B absorbing compounds such as anthocyanin and flavonoids. The antioxidant enzymes were unaffected and showed an enhanced activities of peroxidase, superoxide dismutase, polyphenoloxidase and phenylalanine ammonia-lyase except catalase in UV-B irradiated seedling. Indigofera tinctoria seedling tries to counteract high level of reactive oxygen species produced under UV-B stress through the increased activities of antioxidant enzyme. The results suggest that Indigofera tinctoria is resistant to UV-B radiation damage and the possible negative effect of additional UV-B radiation on the growth of seedling may have been effectively balanced by the UV-B radiation stress through increase in UV-absorbing compound and antioxidant enzymes.

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“…UV damage can be prevented permanently by changing the genetic architecture of genomes, or temporally by regulating the expression of existing genes [ 21 ]. For example, plants can prevent and alleviate UV damage by changing their petiole angle, leaf shape (e.g., curling leaves and shiny wax coating), and leaf size [ 22 ], or by synthesizing particular compounds, such as anthocyanins, antioxidant enzymes, flavonoids and phenolics [ 22 , 23 ]. When exposed to high UV irradiation, fungal species can produce specific pigments, such as melanin [ 24 ], carotenoids and mycosporines [ 25 ] to shield themselves, delay germination, inhibit germ-tube extension [ 26 ], and/or change their ecological niches, such as invading hosts to avoid light [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Altitudinal Heterogeneity of UV Adaptation in Phytophthorainfestans Is Associated with the Spatial Distribution of a DNA Repair Gene

Wang

Waheed

Liu

et al. 2021

JoF

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Climate change is considered a major threat to society and nature. UV irradiation is the most important environmental genotoxic agent. Thus, how elevated UV irradiation may influence human health and ecosystems has generated wide concern in the scientific community, as well as with policy makers and the public in general. In this study, we investigated patterns and mechanisms of UV adaptation in natural ecosystems by studying a gene-specific variation in the potato late blight pathogen, Phytophthora infestans. We compared the sequence characteristics of radiation sensitive 23 (RAD23), a gene involved in the nucleotide excision repair (NER) pathway and UV tolerance, in P. infestans isolates sampled from various altitudes. We found that lower genetic variation in the RAD23 gene was caused by natural selection. The hypothesis that UV irradiation drives this selection was supported by strong correlations between the genomic characteristics and altitudinal origin (historic UV irradiation) of the RAD23 sequences with UV tolerance of the P. infestans isolates. These results indicate that the RAD23 gene plays an important role in the adaptation of P. infestans to UV stress. We also found that different climatic factors could work synergistically to determine the evolutionary adaptation of species, making the influence of climate change on ecological functions and resilience more difficult to predict. Future attention should aim at understanding the collective impact generated by simultaneous change in several climate factors on species adaptation and ecological sustainability, using state of the art technologies such as experimental evolution, genome-wide scanning, and proteomics.

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Protective mechanism in UV-B treated Crotalaria juncea L. seedlings

Cited by 17 publications

References 25 publications

Elicitation of Therapeutic Potential and Oxidative Stress Assessment of Fenugreek Sprouts Under Uv Irradiation

Elicitation of Therapeutic Potential and Oxidative Stress Assessment of Fenugreek Sprouts Under Uv Irradiation

Effect of ultraviolet-B radiation on biochemical and antioxidant defence system in <i>Indigofera tinctoria</i> L. seedlings

Altitudinal Heterogeneity of UV Adaptation in Phytophthorainfestans Is Associated with the Spatial Distribution of a DNA Repair Gene

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