Protective immunity against Eimeria maxima induced by vaccines of Em14-3-3 antigen

Liu, Tingqi; Huang, Jingsheng; Ehsan, Muhammad; Wang, Shuai; Hong, Fei; Zhou, Zhouyang; Song, Xiaoling; Ren, Yan; Xu, Lixin; Li, Xiangrui

doi:10.1016/j.vetpar.2018.02.027

Cited by 20 publications

(14 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Localisation of the proteins is critical to understanding the role which they play in parasite binding and the invasion of the host cell [ 61 , 62 ]. Previous studies reported that monoclonal antibodies were able to detect proteins on the parasite surface, such as EtSAG1 and the micronemes of the sporozoites and merozoites [ 63 – 65 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

et al. 2018

Self Cite

View full text Add to dashboard Cite

BackgroundCoccidiosis is recognised as a major parasitic disease in chickens. Eimeria maxima is considered as a highly immunoprotective species within the Eimeria spp. family that infects chickens. In the present research, the surface antigen gene of E. maxima (EmSAG) was cloned, and the ability of EmSAG to stimulate protection against E. maxima was evaluated.MethodsProkaryotic and eukaryotic plasmids expressing EmSAG were constructed. The EmSAG transcription and expression in vivo was performed based on the RT-PCR and immunoblot analysis. The expression of EmSAG in sporozoites and merozoites was detected through immunofluorescence analyses. The immune protection was assessed based on challenge experiments. Flow cytometry assays were used to determine the T cell subpopulations. The serum antibody and cytokine levels were evaluated by ELISA.ResultsThe open reading frame (ORF) of EmSAG gene contained 645 bp encoding 214 amino acid residues. The immunoblot and RT-PCR analyses indicated that the EmSAG gene were transcribed and expressed in vivo. The EmSAG proteins were expressed in sporozoite and merozoite stages of E. maxima by the immunofluorescence assay. Challenge experiments showed that both pVAX1-SAG and the recombinant EmSAG (rEmSAG) proteins were successful in alleviating jejunal lesions, decreasing loss of body weight and the oocyst ratio. Additionally, these experiments possessed anticoccidial indices (ACI) of more than 170. Higher percentages of CD4+ and CD8+ T cells were detected in both EmSAG-inoculated birds than those of the negative control groups (P < 0.05). The EmSAG-specific antibody concentrations of both the rEmSAG and pVAX1-EmSAG groups were much higher than those of the negative controls (P < 0.05). Higher concentrations of IL-4, IFN-γ, TGF-β1 and IL-17 were observed more in both the rEmSAG protein and pVAX1-SAG inoculated groups than those of negative controls (P < 0.05).ConclusionsOur findings suggest that EmSAG is capable of eliciting a moderate immune protection and could be used as an effective vaccine candidate against E. maxima.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this case, CD8α was significantly downregulated in the intestine of Ross 308 broilers at 6d pi with both infectious doses and CD4 expression was upregulated significantly less, at 13d pi, compared to Ranger Classic broilers. A study by Hong et al reported that E. maxima infection increased both CD8 and CD4 expression at 6‐8 days pi and both CD4 and CD8 lymphocytes numbers, which may have occurred via recognition of antigens such as those encoded by E. maxima surface antigen genes or Eimeria glyceraldehyde‐3‐phosphate dehydrogenase . However, the genotype of these chickens was not reported but the result probably reflects the use of a different genotype to the ones we have used and further highlights the importance of genetic background when extrapolating data between different studies of Eimeria infection in chickens.…”

Section: Discussionmentioning

confidence: 79%

Differential immune response to Eimeria maxima infection in fast‐ and slow‐growing broiler genotypes

Giles

Sakkas

Belkhiri

et al. 2019

Parasite Immunology

View full text Add to dashboard Cite

Very little has been reported comparing resistance to coccidiosis in fast or slow growing broilers, the latter of which are becoming more prevalent in the broiler industry. We examined mRNA expression in the intestines of fast and slow growing broilers following Eimeria infection. We show that by day 13 post‐infection (d pi) with 2500 or 7000 oocysts of Eimeria maxima, slower‐growing (Ranger Classic) broilers significantly (P < 0.01) upregulated expression of proinflammatory cyclooxygenase genes (LTB4DH, PTSG1 and PTSG2) above that detected in fast growing (Ross 308) broilers. Expression of CD8α mRNA was downregulated in Ross 308 at day 6d pi with either 2500 or 7000 oocysts of E maxima (P < 0.05), compared to uninfected controls, but was not differentially expressed in Ranger Classic. CD4 genes were not differentially expressed in either chicken line infected with either infectious oocyst dose at d6 pi, compared to uninfected controls. However, at d13 pi, CD4 expression was significantly upregulated in both chicken lines infected with either infectious oocyst dose, compared to uninfected controls (P < 0.05) but this was significantly greater in Ranger Classic broilers compared to Ross 308 (P < 0.05). At d13 pi, expression of CD3 chains (required for T lymphocyte activation) was significantly increased in Ranger Classic compared to Ross 308, infected with either oocyst dose (P < 0.05‐0.01). Expression of IL‐2 and IL‐15 mRNA, required for T lymphocyte proliferation was also significantly upregulated, or maintained longer, in Ranger Classic broilers compared to Ross 308. These differences in immune response to E maxima corresponded with a reduction in E maxima genome detected in the intestines of Ranger Classic compared to Ross 308.

show abstract

“…Therefore, subunit coccidiosis vaccines, produced using recombinant DNA technology and having the advantage of being safe, cost-effective and efficacious, are urgently needed. In recent years, DNA-based vaccines against coccidiosis have been reported with some protection [10][11][12][13][14]. However, it is well known that field coccidiosis is often caused by co-infections with multiple pathogenic strains of Eimeria [15,16].…”

Section: Introductionmentioning

confidence: 99%

Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina, E. tenella or E. necatrix

Liu

Tuo

et al. 2020

Parasites Vectors

View full text Add to dashboard Cite

Background: Coccidiosis is caused by Eimeria spp. and can result in severe economic losses to the global poultry industry. Due to anticoccidial drug resistance rapidly developing in the parasites and drug residues in poultry products, efficacious and safe alternative coccidia control measures are needed. The objective of the present study was to identify common protective antigens which may be used as vaccine candidates in the development of subunit, multivalent, cross-protective vaccines against most of the economically important Eimeria species.Methods: Whole sporozoite proteins of Eimeria acervulina were prepared and analyzed by 2-dimensional gel electrophoresis (2-DE) followed by western blotting using immune sera specific to E. tenella, E. acervulina, or E. necatrix. The protein spots detected by all three immune sera were then excised from the preparative gel and protein ID was performed by MALDI-TOF-MS/MS. Results:Approximately 620 E. acervulina sporozoite protein spots were demonstrated by 2-DE with silver staining, among which 23 protein spots were recognized by immune sera specific to all three Eimeria species. The results showed that 21 putative E. acervulina proteins were identified, which include proteins with known enzymatic properties, and those which are involved in protein translation, transport and trafficking, and ribosomal biogenesis and functions. There is one protein which may be involved in transcription and one heat-shock protein. Two proteins contain predicted domains, but with no apparent functions known. There were 2 protein spots which had no detectable proteins. None of the proteins has a predicted signal peptide or a transmembrane domain; however, 6 of the 21 putative proteins were predicted to be potentially secretory through the non-classical pathway. Conclusions:Our study identified a diverse group of antigens immunologically common to all three Eimeria species, none of which was previously characterized and tested as a vaccine candidate. Further research on immunogenicity and cross-protective potential of these individual proteins as vaccine candidates will aid the development of vaccines against the most common and pathogenic Eimeria spp.

show abstract

Protective immunity against Eimeria maxima induced by vaccines of Em14-3-3 antigen

Cited by 20 publications

References 50 publications

Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

Differential immune response to Eimeria maxima infection in fast‐ and slow‐growing broiler genotypes

Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina, E. tenella or E. necatrix

Contact Info

Product

Resources

About