Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

Liu, Tingqi; Huang, Jingsheng; Liu, Yanlin; Ehsan, Muhammad; Wang, Shuai; Zhou, Zhouyang; Song, Xiaoling; Ren, Yan; Xu, Lixin; Li, Xiangrui

doi:10.1186/s13071-018-2906-5

Cited by 23 publications

(20 citation statements)

References 64 publications

(63 reference statements)

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“…The reduction in oocyst production was 30–90% using E. tenella or E. acervulina antigens in recombinant protein, DNA or live-vector formulations with or without cytokines as adjuvants [27, 28]. Moreover, the protection against E. tenella , E. necatrix , E. maxima or E. acervulina infection was also reported to be enhanced using multivalent epitope DNA vaccines from multiple antigens compared to a single antigen [29–34]. Although those subunit or recombinant vaccines achieved promising successes in the laboratory and are much safer and less expensive than live anticoccidial vaccines, no large-scale trials have been reported [27].…”

Section: Discussionmentioning

confidence: 99%

Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection

et al. 2019

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Background Live anticoccidial vaccines have been a tremendous success for disease prevention. The establishment of the reverse genetic manipulation platform has enabled the development of Eimeria parasites, the live anticoccidial vaccine strains, as vaccine vectors. In our previous study, recombinant E. tenella expressing a single immunodominant antigen of E. maxima (Et-EmIMP1) was able to protect chickens against challenge infection with E. maxima . This promising result encouraged us to further explore strategies to improve the protection efficacy of recombinant Eimeria and develop it as a vaccine vector. Results We constructed a novel recombinant Eimeria line expressing apical membrane antigen 1 of E. maxima (Et-EmAMA1) and then immunized chickens with Et-EmAMA1 and/or Et-EmIMP1. We found that the E. maxima soluble antigen-specific cell-mediated immunity was much stronger in the birds that were co-immunized with Et-EmAMA1 and Et-EmIMP1 than in those that were immunized with Et-EmAMA1 or Et-EmIMP1 alone. The oocyst production after E. maxima infection was significantly reduced in the recombinant Eimeria -immunized birds compared with the wild-type-immunized and naïve birds. The oocyst production in the birds co-immunized with Et-EmAMA1 and Et-EmIMP1 was consistently the lowest among the treatment groups after E. maxima infection. Conclusions These results demonstrated that Eimeria is an effective vaccine vector that can carry and deliver heterologous Eimeria antigens to the host immune system and trigger specific immune responses. Our results also suggested that increasing the number of recombinant Eimeria lines is an effective approach to enhance protective immunity against infections with heterologous pathogens.

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Section: Discussionmentioning

confidence: 99%

Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection

et al. 2019

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“…Previous studies of Eimeria spp. proteins have shown that a similar effect can be produced after immunization with recombinant protein or recombinant plasmid [ 25 , 26 ]. The data presented here showed that immunization with r Et CHP18905 could produce partial protection against live E. tenella infection.…”

Section: Discussionmentioning

confidence: 99%

“…The concentration of soluble sCD4 and sCD8 in serum is consistent with the number of CD4+and CD8+lymphocytes that produce them [ 41 , 46 ]. Previous studies reported that levels of sCD4 and sCD8 were higher in experimental groups immunized with r Em SAG and r Em MIC7 compared with the control [ 16 , 26 ]. However, the results presented here showed that serum sCD4 and sCD8 were not significantly different in immunized chickens compared to non-immunized controls.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and protective efficacy of a new conserved hypothetical protein ofEimeria tenella

et al. 2021

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Eimeria tenella is an obligate intracellular parasite that actively invades cecal epithelial cells of chickens. This parasite encodes a genome of more than 8000 genes. However, more than 70% of the gene models for this species are currently annotated as hypothetical proteins. In this study, a conserved hypothetical protein gene of E. tenella, designated EtCHP18905, was cloned and identified, and its immune protective effects were evaluated. The open reading frame of EtCHP18905 was 1053bp and encoded a protein of 350 amino acids with a molecular weight of 38.7kDa. The recombinant EtCHP18905 protein (rEtCHP18905) was expressed in E. coli. Using western blot, the recombinant protein was successfully recognized by anti GST-Tag monoclonal antibody and anti-sporozoites protein rabbit serum. Real-time quantitative PCR analysis revealed that the EtCHP18905 mRNA levels were higher in sporozoites than in unsporulated oocysts, sporulated oocysts and second-generation merozoites. Western blot analysis showed that EtCHP18905 protein expression levels were lower in sporozoites than in other stages. Immunofluorescence analysis indicated that the EtCHP18905 protein was located on the surface of sporozoites and second-generation merozoites. Inhibition experiments showed that the ability of sporozoites to invade host cells was significantly decreased after treatment with the anti-rEtCHP18905 polyclonal antibody. Vaccination with rEtCHP18905 protein was able to significantly decrease mean lesion scores and oocyst outputs as compared to non-vaccinated controls. The results suggest that the rEtCHP18905 protein can induce partial immune protection against infection with E. tenella and could be an effective candidate for the development of new vaccines.

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“…The immunofluorescence assay was performed as previously described with several improvements ( Liu et al., 2018 ). Briefly, the purified sporozoites and merozoites were adhered to coverslips precoated with poly-lysine, fixed with 4% (w/v) paraformaldehyde, and permeabilized with 0.1% Triton.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of 4 merozoite antigens as candidate vaccines against Eimeria tenella infection

Song

Zhang

et al. 2021

Poultry Science

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Coccidiosis, caused by parasites of the genus Eimeria , is one of the most widespread and economically detrimental diseases in the global poultry industry. Because the merozoite stage of Eimeria tenella is immunologically vulnerable, motile, and functionally important for the parasites, the proteins expressed in these stages are considered to be potentially immunoprotective antigens, especially the secreted antigens and surface antigens. Here, we detected a previously unidentified MIC2-associated protein (Et-M2AP) from E. tenella and determined its localization. An immunofluorescence assay revealed that Et-M2AP was distributed in the apical part of second generation merozoites and sporozoites. In addition, an expression profile analysis revealed that the transcriptional level of Et-M2AP is significantly higher in the merozoite stage. To assess the potential of Et-M2AP protein as a coccidiosis vaccine, we expressed recombinant Et-M2AP ( rEt-M2AP ) and compared the immune protective efficacy of rEt-M2AP with 3 surface antigens that are highly expressed by merozoites (rEt-SAG23, rEt-SAG16, and rEt-SAG2 proteins). The immune protective efficacy of these vaccine candidates was assessed based on survival rate, lesion score, BW gain, relative BW gain, and oocyst output. The results show that the survival rate was 90%, which are significantly higher than those in the challenge control group. The BW gain rate was 42% ( P < 0.001) in rEt-M2AP–immunized chickens, which are significantly higher than those in the challenge control group and rEt-SAG23, rEt-SAG16, and rEt-SAG2 proteins–immunized chickens. In addition, chickens immunized with rEt-M2AP (88% oocyst output decrease rate, P < 0.001) had the least oocyst output, compared with those immunized with rEt-SAG16 (59.2% oocyst output decrease rate, P < 0.001), rEt-SAG23 (22% oocyst output decrease rate), and rEt-SAG2 (1.36% oocyst output decrease rate). These results demonstrate that rEt-M2AP provided effective protection against challenge with E. tenella , suggesting that rEt-M2AP is a promising candidate antigen gene for development as a coccidiosis vaccine.

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Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

Cited by 23 publications

References 64 publications

Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection

Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection

Molecular characterization and protective efficacy of a new conserved hypothetical protein ofEimeria tenella

Evaluation of 4 merozoite antigens as candidate vaccines against Eimeria tenella infection

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