Protective efficacy of recombinant OmpTS protein of Aeromonas hydrophila in Indian major carp

Enhancement of protective immunity in European eel (<italic>Anguilla anguilla</italic>) against <italic>Aeromonas hydrophila</italic> and <italic>Aeromonas sobria</italic> by a recombinant <italic>Aeromonas</italic> outer membrane protein

“…Each column represents mean log 2 value + SD of the highest dilution/50 of the serum because of the least serum dilution was 1:50 (n ¼ 6). not been done [19]. In this study, we constituted a recombinant OMP (rOmp-G), which provided protective immunogenicity for European eels to defend themselves against one A. sobria and two different serotypes of A. hydrophila strains at the same time [ Fig.…”

“…The OMPs can induce specific bactericidal antibodies [8][9][10], inhibit the bacterial colonization in hosts [11], and induce cell-mediated immunity [12][13][14]. Many OMPs from different pathogenic bacteria, such as Aeromonas salmonicida [8, [15][16][17], Aeromonas hydrophila [9,[18][19][20][21], Vibrio parahaemolyticus [22], Vibrio alginolyticus [23], Vibrio harveyi [12 -13], Edwardsiella ictaluri [10], and Edwardsiella tarda [14,24], have been identified as effective protective antigens. Some OMPs mixtures extracted from one pathogenic bacterial strain have been proved to provide cross protection for fish against several other bacterial strains or species [15,20], suggesting that different pathogenic bacterial species may share some similar or conserved OMP antigens.…”

An outer membrane protein, OmpK, is an effective vaccine candidate for Vibrio harveyi in Orange-spotted grouper (Epinephelus coioides)

“…However, obtaining the OMP in large quantity is labor intensive and costly, while recombinant expression of the gene in vitro can overcome this problem. Recombinant ompTS has already been shown to give partial protection against challenge with A. hydrophila [24,25]. In this work, the OmpK gene of V. harveyi was cloned and expressed in E. coli, and it was shown that the recombinant protein of OmpK was a protective antigen for V. harveyi in Orangespotted groupers.…”

“…Wua chicken [30]. With regard to fish disease organisms, Khushiramani et al reported that recombinant OmpTS of A. hydrophila provided protection for Indian major carp (Labeo rohita) and its RPS was 57 [25]. Recently it was proved that recombinant OmpK of V. parahaemolyticus could provide protection for large yellow croaker and its RPS was 90 [17].…”

Heterologous Soluble Expression of Recombinant OmpR of <i>Aeromonas hydrophila</i> and Its Immunogenic Potential

“…The outer membrane/surface proteins have been considered to be ideal candidates for vaccine development. Recently, recombinant outer membrane proteins OmpTS, OmpA and OmpW of A. hydrophila have been investigated for their immunogenic potential [11]- [13].…”