An outer membrane protein, OmpK, is an effective vaccine candidate for Vibrio harveyi in Orange-spotted grouper (Epinephelus coioides)

Identification of novel vaccine candidates againstAcinetobacter baumanniiusing reverse vaccinology

“…38 The Nlp gene of Yersinia pestisnlp D (similar to ABAYE1048) encoding for outer membrane lipoprotein could elicit a protective immunity against Y. pestis.…”

“…Therefore, 3 potential proteins OmpK, FKIB and Ompp1 corresponding to ABAYE0130, ABAYE3820 and ABAYE0711, respectively which were selected had the highest antigenicity scores 0.73, 0.66 and 0.65, respectively ( Table 2). These antigens also are potential vaccine candidates in other bacterial pathogens 38,44,50,51 and should be evaluated for their protective ability in mouse immunogenicity studies. The protein sequence of each antigen from 14 published A. baumannii genomes was aligned by BlastP to obtain a consensus sequence, and the gene fragment of the identified consensus sequences was synthesized using the codon-optimization for E. coli expression.…”

Selection of specific cell wall antigen for rapid detection of fish pathogenic Vibrio parahaemolyticus by enzyme immunoassay

“…However it is necessary, to select appropriate antigens for developing immunoassays, in order to avoid cross reactions. For Gram negative bacteria, the selection of appropriate antigen is somewhat easier, as different proteins from the outer membranes are known to be common antigens for a genus or a species (Fofstra and Dankert, 1979;Inoue et al, 1995;Ningqiu et al, 2008). The outer membranes of Gram negative bacteria contain several distinct proteins, with molecular weights in the range of 30 to 40 kDa (Manning et al, 1982;Li et al, 2010).…”

Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain

“…Several V. harveyi recombinant subunit vaccine candidates have been reported by different research groups and demonstrated to be effective in laboratory trials. These protein-based vaccines include the outer membrane protein OmpK, glyceraldehyde-3-phosphate dehydrogenase, and haemolysin [28][29][30]. In addition, our recent study has identified a chaperon protease from V. harveyi and found it highly immunoprotective when used as a secreted vaccine [31].…”