Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain

Cheng, Shuang; Zhang, Weiwei; Zhang, Min; Sun, Li

doi:10.1016/j.vaccine.2009.10.122

Cited by 31 publications

(19 citation statements)

References 45 publications

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“…Whole-cell proteins were prepared by lysing the cells in lysis buffer as described previously [27]. The concentration of the proteins was determined using the Bradford method with bovine serum albumin (BSA) as the standard.…”

Section: Whole-cell Protein Analysismentioning

confidence: 99%

Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus

Deng

Sun

et al. 2012

Fish & Shellfish Immunology

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Section: Whole-cell Protein Analysismentioning

confidence: 99%

Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus

Deng

Sun

et al. 2012

Fish & Shellfish Immunology

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“…Fish were fed daily with commercial dry pellets (purchased from Shandong Sheng-suo Fish Feed Research Center, Shandong, China). Before experimental manipulation, the health status of the fish was examined by randomly sampling five fish from each tank and examined for bacterial recovery from blood, liver, spleen, and kidney as described previously [24]. Fish were considered healthy and used for experiments only when no bacteria could be detected from any of the sampled fish.…”

Section: Fishmentioning

confidence: 99%

Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus

Zhang¹,

Xiao²,

Sun³

2011

Fish & Shellfish Immunology

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“…Whole-cell proteins were prepared by lysing the cells in lysis buffer as described previously (Cheng et al 2010). The concentration of the proteins was determined using the Bradford method with bovine serum albumin (BSA) as the standard.…”

Section: Whole-cell Protein Productionmentioning

confidence: 99%

C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine

Yu¹,

Hu²,

Sun³

et al. 2012

Dis. Aquat. Org.

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Vibrio anguillarum, a Gram-negative bacterial pathogen, is the causative agent of vibriosis that affects a wide range of aquatic animals. In this study, we obtained a mutant V. anguillarum, C312M, derived from the pathogenic V. anguillarum C312 by selection of rifampicin resistance. C312M was slower in growth than the wild type C312, particularly under conditions of iron depletion. Compared to C312, C312M was altered in protein production profile and exhibited a dramatically increased median lethal dose. Safety analysis showed that C312M was stable in virulence in the absence of selective pressure. To examine the potential of C312M as a live attenuated vaccine, Japanese flounder Paralichthys olivaceus were vaccinated with C312M via oral, immersion, and oral plus immersion routes. Microbiological analysis showed that C312M was recovered from the gut, liver, kidney, and spleen of the vaccinated fish in 1 to 14 d post-vaccination. When the fish were challenged with C312 at 1 mo post-vaccination, C312M-vaccinated fish exhibited relative percent survival rates of 60 to 84%. Comparable protection was observed when the fish were challenged with a heterologous V. anguillarum strain. Further analysis showed that C312M-vaccinated fish produced specific serum antibodies which enhanced serum bactericidal activity in a manner that is probably complement-dependent. These results indicate that C312M is highly attenuated in virulence but still retains residual infectivity, and that C312M is an effective vaccine when delivered alive via immersion and oral feeding.

show abstract

Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain

Cited by 31 publications

References 45 publications

Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus

Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus

Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus

C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine

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