Protective efficacy of crocetin and its nanoformulation against cyclosporine A-mediated toxicity in human embryonic kidney cells

Pradhan, Jyotsnamayee; Mohanty, Chandana; Sahoo, Sanjeeb K.

doi:10.1016/j.lfs.2018.11.027

Cited by 10 publications

(6 citation statements)

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“…In a previous study, pre-treatment of bovine aortic endothelial cells with crocetin (1 M) for 12 h revealed that it reduced ROS formation (Xiang et al 2006). Additionally, crocetin (0.1, 0.5, and 1 µM, for 24 h) was found to enhance antioxidant enzymes, leading to the detoxi cation of free radicals in cyclosporine A-mediated cytotoxic in HEK-2 cells (Pradhan et al 2019). According to previous research projects, we realize that oxidative stress has a basic and important gure in MB toxicity in HEK-2 cells.…”

Section: Discussionmentioning

confidence: 98%

“…RM leads to the release of cellular contents including enzymes, MB, and electrolytes into the extracellular uid ( TSC is an effective antioxidant that reduces intracellular ROS (Stennett et al 2007). It enhances the activity of the internal antioxidant enzymes and clears free radicals (Pradhan et al 2019). In a previous study, pre-treatment of bovine aortic endothelial cells with crocetin (1 M) for 12 h revealed that it reduced ROS formation (Xiang et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Also, in HEK-2 cells, crocetin repaired the antioxidant system and prevented ROS production. Likewise, it prevented the ROS release from mitochondria into the cytoplasm through the steadiness of the mitochondrial membrane potential (Pradhan et al 2019). Numerous lines of documents reported the advantageous effects of crocetin as a potent antioxidant in the protection of kidney diseases (Bandegi et al 2014).…”

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confidence: 99%

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Trans-sodium crocetinate suppresses apoptotic and oxidative response following myoglobin-induced cytotoxicity in HEK-2 cells

Aminifard

Mehri

Rahbardar

et al. 2023

Preprint

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Purpose: Rhabdomyolysis (RM) is a serious fatal syndrome. The RM leads to acute kidney injury (AKI) as a fatal complication. The belief is that the RM-induced AKI is triggered by myoglobin (MB). MB activates oxidative and apoptotic pathways.Trans-sodium crocetinate (TSC) isobtained from saffron and it has antioxidant andrenoprotective effects. This research was designed to assess the mechanisms of MB-induced cytotoxicity in HEK-2 cells (human embryonic kidney cells) as well as the possible effects of TSC against MB-induced cytotoxicity. Methods: HEK-2 cells were exposed to diverse concentrations of TSC (2.5, 5, 10, 20, 40, 80, and 100 µM) for 24 h. Then, MB (9 mg/ml) was added to the cells. After 24 h, cell viability was measured through MTT, and The values of ROS generation were calculated using DCFH-DA assay. Besides, using western blot autophagy and apoptosis markers in cells were assessed. Results: MB decreased viability and increased ROS levels in HEK-2 cells. Furthermore, MB enhanced the apoptosis markers (cleaved caspase-3 and Bax/Bcl-2 ratio) and autophagy markers (LC3II/I ratio and Beclin-1) in HEK-2 cells. However, pretreatment of HEK-2 cells with TSC for 24 h reduced the cytotoxicity and ROS production caused by MB. Additionally, TSC pretreatment condensed the levels of autophagy and apoptosis markers in response to MB cytotoxicity. Conclusion:TSC has a positive effect in preventing MB-induced cytotoxicity in HEK-2 cells by increasing antioxidant activity and regulation of apoptotic and autophagy signaling pathways.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Trans-sodium crocetinate suppresses apoptotic and oxidative response following myoglobin-induced cytotoxicity in HEK-2 cells

Aminifard

Mehri

Rahbardar

et al. 2023

Preprint

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“…The reactive ‧OH radicals produced by Fenton's reagent during the in vitro DNA nicking assay can mimic biological systems involving DNA damage. Protective effects of antioxidants against DNA strand breakage can be useful for attenuating oxidative DNA damage and help to protect from various human diseases (Pradhan, Mohanty, & Sahoo, 2019). In the current experiment, it was explored that vanillic acid possesses the effective anti‐oxidant potential to avoid DNA damage against hydroxyl radicals.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of vanillic acid against diabetes and diabetic nephropathy by attenuating oxidative stress and upregulation of NF‐κB, TNF‐α and COX‐2 proteins in rats

Singh

Kumar

Singh

et al. 2022

Phytotherapy Research

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Diabetes is the most prevalent disorder in the world characterized by uncontrolled high blood glucose levels and nephropathy is one of the chief complications allied with hyperglycemia. Vanillic acid; the main bioactive compound derived from natural sources such as vegetables, fruits and plants possesses various pharmacological activities such as antioxidant, anti-inflammatory and anti-proliferative. The current study was designed to investigate the antidiabetic and renoprotective effects of vanillic acid by its various pharmacological activities. Streptozotocin (50 mg/kg)/nicotinamide (110 mg/ kg) was used to induce diabetes in rats. Oral administration of vanillic acid once daily for 6 weeks (25, 50 and 100 mg/kg) significantly reduced the hyperglycemia, increased

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“…However, crocetin is insoluble in water [ 27 ], limiting the oral bioavailability and therapeutic application. Recently, different nanoparticles were studied to improve crocetin’s solubility and efficacy [ 28 , 29 , 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Crocetin as New Cross-Linker for Bioactive Sericin Nanoparticles

et al. 2021

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The nose-to-brain delivery route is used to bypass the blood–brain barrier and deliver drugs directly into the brain. Over the years, significant signs of progress have been made in developing nano-drug delivery systems to address the very low drug transfer levels seen with conventional formulations (e.g., nasal solutions). In this paper, sericin nanoparticles were prepared using crocetin as a new bioactive natural cross-linker (NPc) and compared to sericin nanoparticles prepared with glutaraldehyde (NPg). The mean diameter of NPc and NPg was about 248 and 225 nm, respectively, and suitable for nose-to-brain delivery. The morphological investigation revealed that NPc are spherical-like particles with a smooth surface, whereas NPg seem small and rough. NPc remained stable at 4 °C for 28 days, and when freeze-dried with 0.1% w/v of trehalose, the aggregation was prevented. The use of crocetin as a natural cross-linker significantly improved the in vitro ROS-scavenging ability of NPc with respect to NPg. Both formulations were cytocompatible at all the concentrations tested on human fibroblasts and Caco-2 cells and protected them against oxidative stress damage. In detail, for NPc, the concentration of 400 µg/mL resulted in the most promising to maintain the cell metabolic activity of fibroblasts higher than 90%. Overall, the results reported in this paper support the employment of NPc as a nose-to-brain drug delivery system, as the brain targeting of antioxidants is a potential tool for the therapy of neurological diseases.

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Protective efficacy of crocetin and its nanoformulation against cyclosporine A-mediated toxicity in human embryonic kidney cells

Cited by 10 publications

References 50 publications

Trans-sodium crocetinate suppresses apoptotic and oxidative response following myoglobin-induced cytotoxicity in HEK-2 cells

Trans-sodium crocetinate suppresses apoptotic and oxidative response following myoglobin-induced cytotoxicity in HEK-2 cells

Protective effect of vanillic acid against diabetes and diabetic nephropathy by attenuating oxidative stress and upregulation of NF‐κB, TNF‐α and COX‐2 proteins in rats

Crocetin as New Cross-Linker for Bioactive Sericin Nanoparticles

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