Protective effect of vanillic acid against diabetes and diabetic nephropathy by attenuating oxidative stress and upregulation of <scp>NF‐κB</scp>, <scp>TNF</scp>‐α and <scp>COX</scp>‐2 proteins in rats

Singh, B. N.; Kumar, Ajay; Singh, Hardeep; Kaur, Sarabjit; Arora, Saroj; Singh, Balbir

doi:10.1002/ptr.7392

Cited by 45 publications

(30 citation statements)

References 67 publications

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“…Diabetes was induced in laboratory animals either by streptozotocin (STZ) or by alloxan. O. basilicum extracts and EOs revealed several mechanisms of diabetes management, such as modulation of biochemical, morphometric, and histological disorders of colonic tissue, increased activities of plasma ALT, lysozyme, and total protein, decreased levels of triglycerides, plasma glucose, and hepatic glycogen [ 93 , 94 , 95 ]. From the phytochemical section of our work and the results of Mousavi et al [ 96 ], basil showed richness in active polyphenolic components such as rosmarinic acid, azulene, luteolin, kaempferol, genistein, caffeic acid, diosmetin, 3,4-dimethoxycinnamic acid, and apigenin, which are known to control blood glucose in diabetic animals.…”

Section: Discussionmentioning

confidence: 99%

Determination of Chemical Composition and Investigation of Biological Activities of Ocimum basilicum L.

Qasem

Assaggaf

Mrabti³

et al. 2023

Molecules

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This study aimed to determine the chemical composition of the essential oils (EOs) of Ocimum basilicum L., as well as to evaluate the antibacterial, antidiabetic, dermatoprotective, and anti-inflammatory properties, and the EOs and aqueous extracts of O. basilicum. The antibacterial activity was evaluated against bacterial strains, Gram-positive and Gram-negative, using the well diffusion and microdilution methods, whereas the antidiabetic activity was assessed in vitro using two enzymes involved in carbohydrate digestion, α-amylase and α-glucosidase. On the other hand, the dermatoprotective and anti-inflammatory activities were studied by testing tyrosinase and lipoxygenase inhibition activity, respectively. The results showed that the chemical composition of O. basilicum EO (OBEO) is dominated by methyl chavicol (86%) and trans-anethol (8%). OBEO exhibited significant antibacterial effects against Gram-negative and Gram-positive strains, demonstrated by considerable diameters of the inhibition zones and lower MIC and MBC values. In addition, OBEO exhibited significant inhibition of α-amylase (IC50 = 50.51 ± 0.32 μg/mL) and α-glucosidase (IC50 = 39.84 ± 1.2 μg/mL). Concerning the anti-inflammatory activity, OBEO significantly inhibited lipoxygenase activity (IC50 = 18.28 ± 0.03 μg/mL) compared to the aqueous extract (IC50 = 24.8 ± 0.01 μg/mL). Moreover, tyrosinase was considerably inhibited by OBEO (IC50 = 68.58 ± 0.03 μg/mL) compared to the aqueous extract (IC50 = 118.37 ± 0.05 μg/mL). The toxicological investigations revealed the safety of O. basilicum in acute and chronic toxicity. The finding of in silico analysis showed that methyl chavicol and trans-anethole (main compounds of OBEO) validate the pharmacokinetics of these compounds and decipher some antibacterial targets.

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Section: Discussionmentioning

confidence: 99%

Determination of Chemical Composition and Investigation of Biological Activities of Ocimum basilicum L.

Qasem

Assaggaf

Mrabti³

et al. 2023

Molecules

View full text Add to dashboard Cite

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“…In GAME, HPLC-DAD analysis showed the presence of three major phenolic compounds in GAME, namely: quercetin 7-O-glucoside, vanillic acid, and apigenin. Singh et al [ 23 ] have shown that the oral administration of vanillic acid to diabetic rats significantly reduced the hyperglycemia, increased liver enzymes, and normalized lipid profile that was altered in diabetic rats. Moreover, vanillic acid attenuated the impaired renal function as evidenced by a reduction in serum creatinine, urea, uric acid, and urinary microproteinuria levels with a concomitant increase in urinary creatinine clearance in the nephropathic rats [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…Singh et al [ 23 ] have shown that the oral administration of vanillic acid to diabetic rats significantly reduced the hyperglycemia, increased liver enzymes, and normalized lipid profile that was altered in diabetic rats. Moreover, vanillic acid attenuated the impaired renal function as evidenced by a reduction in serum creatinine, urea, uric acid, and urinary microproteinuria levels with a concomitant increase in urinary creatinine clearance in the nephropathic rats [ 23 ]. In addition, apigenin administration to diabetic rats decreased the blood glucose level significantly [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Globularia alypum Extracts Attenuate Hyperglycemia and Protect against Various Organ Toxicities in Alloxan-Induced Experimental Diabetic Rats

Tiss

Hamden

2022

Evidence-Based Complementary and Alternative Medicine

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In this study, we attempted for the first time to determine the phytochemical compositions and biopharmaceutical properties of Globularia alypum methanol extract (GAME) and Globularia alypum water extract (GAWE). High-performance liquid chromatography with diode array detection (HPLC-DAD) analysis was performed to establish the chemical profile of the investigated extracts. Chemical composition analysis was taken in the presence of various bioactive compounds such as quercetin 7-O-glucoside and apigenin 7-O-glucoside in GAME. In GAWE, various abundant compounds were found in the extract such as quercetin 7-O-glucoside, apigenin, quercetin, apigenin 7-O-glucoside, and cinnamic acid. This study showed that the administration of GAWE and GAME to type 1 diabetic rats decreased fasting blood glucose, protected pancreas β-cells from death and injury, increased liver glycogen rate, and ameliorated oral glucose tolerance test. Moreover, GA reduced weight loss, and diabetes decreased basic physical activity. In addition, the administration of GA extracts in diabetic rats protected from diabetes-induced liver, kidney, testes, heart, and bone toxicities. Conclusion. GAWE has possible value for antidiabetic oral medication.

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“…PTGS2 plays an essential role in the process of oxidative stress [ 44 ], and COX-2, encoded by PTGS2, is a key regulator of renal hemodynamics and promoter of inflammation, which also has a role in podocyte injury. It has been documented that the expression of PTGS2 in podocytes increased significantly under high glucose conditions [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Oxidative Stress-Related Biomarkers in Diabetic Kidney Disease

Zhang

Leng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Diabetic kidney disease (DKD) is a leading cause of end-stage renal disease throughout the world. In kidney disease, oxidative stress has been linked to both antioxidant depletions and increased reactive oxygen species (ROS) production. Thus, the objective of this study was to identify biomarkers related to oxidative stress in DKD. Methods. The gene expression profile of the DKD was extracted from the Gene Expression Omnibus (GEO) database. The identification of the differentially expressed genes (DEGs) was performed using the “limma” R package, and weighted gene coexpression network analysis (WGCNA) was used to find the gene modules that were most related to DKD. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was performed using “Org.Hs.eg.db” R package. The protein-protein interaction (PPI) network was constructed using the STRING database. The hub genes were identified by the Molecular Complex Detection (MCODE) plug-in of Cytoscape software. The diagnostic capacity of hub genes was verified using the receiver operating characteristic (ROC) curve. Correlations between diagnostic genes were analyzed using the “corrplot” package. In addition, the miRNA gene transcription factor (TF) network was used to explain the regulatory mechanism of hub genes in DKD. Results. DEGs analysis and WGCNA-identified 160 key genes were identified in DKD patients. Among them, nine oxidative stress-related genes were identified as candidate hub genes for DKD. Using the PPI network, five hub genes, NR4A2, DUSP1, FOS, JUN, and PTGS2, were subsequently identified. All the hub genes were downregulated in DKD and had a high diagnostic value of DKD. The regulatory mechanism of hub genes was analyzed from the miRNA gene-TF network. Conclusion. Our study identified NR4A2, DUSP1, FOS, JUN, and PTGS2 as hub genes of DKD. These genes may serve as potential therapeutic targets for DKD patients.

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Protective effect of vanillic acid against diabetes and diabetic nephropathy by attenuating oxidative stress and upregulation of NF‐κB, TNF‐α and COX‐2 proteins in rats

Cited by 45 publications

References 67 publications

Determination of Chemical Composition and Investigation of Biological Activities of Ocimum basilicum L.

Determination of Chemical Composition and Investigation of Biological Activities of Ocimum basilicum L.

Globularia alypum Extracts Attenuate Hyperglycemia and Protect against Various Organ Toxicities in Alloxan-Induced Experimental Diabetic Rats

Identification of Oxidative Stress-Related Biomarkers in Diabetic Kidney Disease

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