Protective efficacy of adenovirus-mediated small interfering RNAs against encephalomyocarditis virus challenge in mice

Bai, Juan; Jiang, Kangfu; Zhang, Lili; Wang, Xianwei; Li, Yufeng; Jiang, Ping

doi:10.1016/j.jviromet.2012.07.004

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…13 However, antiviral strategies against EMCV are very limited. [15][16][17] Our previous research demonstrated that bamboo vinegar from Moso bamboo showed strong antiviral activity against EMCV because of the synergistic effect of phenol with acetic acid. 18 The composition and antiviral activity of WV depend on a number of factors that include the plant species and the pyrolysis conditions.…”

Section: Introductionmentioning

confidence: 99%

Structure-dependent antiviral activity of catechol derivatives in pyroligneous acid against the encephalomycarditis virus

Narita

Ouda

et al. 2018

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Structure-dependent antiviral activity of catechol derivatives in pyroligneous acid against the encephalomycarditis virus

Narita

Ouda

et al. 2018

RSC Adv.

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“…VP1 is one of the most important structures proteins of EMCV, which can stimulate the organism to produce neutralization antibody [18]. Immunization of mice with recombinant VP1 protein (plasmids or recombinant adenoviruses expressing small hairpin RNAs targeted to VP1 protein genes of EMCV), which could provide protective efficacy against EMCV [26,27]. DNA vaccines encoding an EMCV-D VP1 antigen confer protective immunity against heterologous challenge with EMCV-K, indicating that VP1 is a potential vaccine candidate for controlling EMCV infection [28].…”

Section: Discussionmentioning

confidence: 99%

Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine

Bai

Chen

Jiang

et al. 2014

Virol J

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BackgroundEncephalomyocarditis virus (EMCV) can cause myocarditis, respiratory failure, reproductive failure, and sudden death in pre-weaned piglets, which has been isolated in China. EMCV VP1 protein was one of the most important structural proteins and played an important role in the protective immunity. In this study, 10 monoclonal antibodies (McAbs) against EMCV VP1 were screened and identified.ResultsEpitope mapping results indicated that McAbs (6E11, 7A7, 7C9) specifically recognized the linear epitopes V(2)ENAEK(7), McAbs (1D1, 2A2, 5A1, 5A11, 5G1) recognized the epitope F(19)VAQPVY(25), and McAbs 1G8 and 3A9 recognized P(42)IGAFTVK(49). Protein sequence alignment of VP1 with 16 EMCV isolates indicated that the epitope F(19)VAQPVY(25) was conserved in all the reference strains. The epitopes P(42)IGAFTVK(49) and V(2)ENAEK(7) only had 1 or 2 variable amino acid among the reference strains. The 3D model analysis results showed that these epitopes presented as spheres were shown within the context of the complete particle.ConclusionsIn this study, ten McAbs against EMCV VP1 were developed and three B-cells epitopes (2-7aa, 19-25aa and 42-49aa) were defined in VP1. All the results herein will promote the future investigations into the function of VP1 of EMCV and development of diagnostic methods of EMCV.

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Construction and characterization of an infectious cDNA clone of encephalomyocarditis virus from pigs in China

et al. 2014

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Encephalomyocarditis virus (EMCV) infects animals of various species and causes a variety of clinical symptoms. In this study, an infectious full-length cDNA clone was constructed, and the characteristics of the rescued virus were investigated in vitro and in vivo. Our data demonstrated that the growth kinetics in vitro and plaque morphology of the rescued EMCV rNJ08 strain were similar to those of the parental strain. Although rNJ08 infected BALB/c mice, none of the mice died during the observation period of 14 days post-inoculation. The availability of the infectious cDNA clone provides a genetic platform for studying gene function and for the rational design of vaccines.

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Protective efficacy of adenovirus-mediated small interfering RNAs against encephalomyocarditis virus challenge in mice

Cited by 4 publications

References 31 publications

Structure-dependent antiviral activity of catechol derivatives in pyroligneous acid against the encephalomycarditis virus

Structure-dependent antiviral activity of catechol derivatives in pyroligneous acid against the encephalomycarditis virus

Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine

Construction and characterization of an infectious cDNA clone of encephalomyocarditis virus from pigs in China

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