Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine

Bai, Juan; Chen, Xinhui; Jiang, Kangfu; Zeshan, Basit; Jiang, Ping

doi:10.1186/s12985-014-0226-8

Cited by 6 publications

(4 citation statements)

References 31 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We clearly understand that the application of vaccination is a key component and measurement in the control and prevention of the disease [17]. However, the present traditional vaccines have several shortcomings, including the need for expensive biological containment facilities, the escape of the virus from improper inactivation, which results in vaccinated animals becoming carriers due to residual live virus, and so on [18]. Thus, the subunit vaccine design and vaccination practices against FMDV are recently blooming as an alternative approach to solve the long-lived classical problems associated with the present vaccine.…”

Section: Discussionmentioning

confidence: 99%

“…It has recently been reported that FMDV VP3 inhibits the expression of type I and type II interferons by reducing the mRNA levels of the virus-induced signal adapter (VISA) and degrading the JAK1 protein [2,34]. Likewise, Li et al [18] reported that VP3 could inhibit IFN-γ induced responses and disrupts the assembly of the JAK1 complex and degrades JAK1 via a lysosomal pathway. Generally, our results confirm that the three fusion proteins could induce the secretion of certain cytokines in mice, such as IFN-γ, IL-2, and IL-4, which could promote an effective cellular immunity against the SAT2 FMDV virus.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus

Wubshet

Ding

et al. 2021

Viruses

View full text Add to dashboard Cite

An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with His-SUMO (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus

Wubshet

Ding

et al. 2021

Viruses

View full text Add to dashboard Cite

show abstract

“…Phylogenetic investigation was based on 1D gene, according to the assumption that VP1 might be subjected to selective pressure and variability. In Picornaviruses, VP1 is an external exposed capsid protein and is involved in viral pathogenicity, receptor binding and immune modulation 45 – 47 . Therefore comparing sequences of this region obtained from different isolates might give exhaustive information about evolution of EMCV in the considered period.…”

Section: Discussionmentioning

confidence: 99%

A recent view about encephalomyocarditis virus circulating in compartmentalised animal population in Northern Italy

Foglia

Pezzoni

Bonilauri

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Encephalomyocarditis virus (Picornaviridae, Cardiovirus A) is the causative agent of the homonymous disease, which may induce myocarditis, encephalitis and reproductive disorders in various mammals, especially in swine. Despite the disease occurred endemically in pig farms since 1997, the recent increase of death experimented in Northern Italy prompted to furtherly investigate the evolution of the virus and the actual spread of the infection. Italian EMC viruses, collected between 2013 and 2019, showed an overall antigenic stability. The in-house ELISA Monoclonal Antibodies based, able to reveal changes in seven different antigenic sites, showed only sporadic and occasional mutations in considered samples and the subsequent phylogenetic analysis confirmed antigenic panel’s remarks. All the isolates could be classified within a unique lineage, which comprise other European strains and confirm that the viruses currently circulating in Italy developed from a unique common ancestor. Despite the demonstrated stability of virus, some putative newly emerged variants were detected through antigenic profile analysis and phylogenesis. Finally, the serosurvey proved that spread of EMCV is greater than the diffusion of fatal infections would suggest, due to subclinical circulation of EMCV. It demonstrated an increase in the proportion of seropositive farms, if compared with previous data with no remarkable differences between farms with and without clinical evidence of disease.

show abstract

“…Therefore, an in-depth understanding of EMCV has important implications for public health (Oberste et al, 2009). EMCV life cycle and molecular epidemiology are well studied (Bai et al, 2014; Feng et al, 2015, 2015; Liu et al, 2016; Luo et al, 2017; Zhang et al, 2017). However, little is known about the factors that influence EMCV replication.…”

Section: Introductionmentioning

confidence: 99%

Transmembrane Protein 39A Promotes the Replication of Encephalomyocarditis Virus via Autophagy Pathway

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

Encephalomyocarditis virus (EMCV) causes encephalitis, myocarditis, neuropathy, reproductive disorders, and diabetes in animals. EMCV is known to induce cell autophagy; however, the molecular mechanisms underlying this remain unclear. Here, we show that the type III-transmembrane protein, transmembrane protein 39A (TMEM39A), plays a critical role in EMCV replication. We showed that EMCV GS01 strain infection upregulated TMEM39A expression. Importantly, EMCV induced autophagy in a range of host cells. The autophagy chemical inhibitor, 3-MA, inhibited EMCV replication and reduced TMEM39A expression. This is the first study demonstrating TMEM39A promoting the replication of EMCV via autophagy. Overall, we show that TMEM39A plays a positive regulatory role in EMCV proliferation and that TMEM39A expression is dependent on the autophagy pathway.

show abstract

Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine

Cited by 6 publications

References 31 publications

Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus

Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus

A recent view about encephalomyocarditis virus circulating in compartmentalised animal population in Northern Italy

Transmembrane Protein 39A Promotes the Replication of Encephalomyocarditis Virus via Autophagy Pathway

Contact Info

Product

Resources

About