Protective effects of unsaponifiable matter from rice bran on oxidative damage by modulating antioxidant enzyme activities in HepG2 cells

Ham, Hyeonmi; Yoon, Sung Won; Kim, In Hwan; Kwak, Jieun; Lee, Jeom Sig; Jeong, Heon Sang; Lee, Junsoo

doi:10.1016/j.lwt.2014.12.047

Cited by 30 publications

(33 citation statements)

References 49 publications

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“…Alteration in the activity of antioxidant enzymes can be regarded as a sensitive marker of the response of cells to oxidative stress (35). Aiming to disclose the effects of furan and lycopene, we determined alterations in intracellular antioxidant enzyme activities.…”

Section: Figure 6 (A) Liver Section Of Control Rats Cv: Central Veinmentioning

confidence: 99%

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Furan-induced hepatotoxic and hematologic changes in diabetic rats: the protective role of lycopene

Baş

Pandır

Kalender

2016

Archives of Industrial Hygiene and Toxicology

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Furan forms as a result of thermal treatment of food and induces harmful effects on organisms. In our work, lycopene, furan, and a combination of the two were given to diabetic male rats for 28 days. Hematological changes, total protein and cholesterol, triglyceride, and albumin levels, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and alkaline phosphatase activities of the serum, malondialdehyde levels, glutathione peroxidase, catalase, glutathione-S-transferase, superoxide dismutase activities, DNA damage in liver tissues and hepatic histopathological alterations were compared to a control group. There were significant changes in the liver function tests, DNA damage, activities of antioxidant enzymes, and malondialdehyde levels between diabetic control and non-diabetic control groups, between diabetic control and diabetic lycopene groups, and also between diabetic furan and diabetic control groups. In diabetic lycopene and diabetic furan + lycopene treated groups we designated the preventive effects of lycopene against diabetes and furan, however, on the analysed parameters only. In spite of some pathological alterations designated in diabetic furan treated group's liver, fewer pathological alterations were observed in furan+lycopene treated groups at the end of week 4. Consequently, lycopene significantly reduced furan-and diabetes-induced toxicity in rat liver.

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Section: Figure 6 (A) Liver Section Of Control Rats Cv: Central Veinmentioning

confidence: 99%

“…Even so, these protective systems cannot exactly oppose the destructive effects of ROS (35). Additional antioxidants can advance to increase the defence systems of cells.…”

Section: Figure 6 (A) Liver Section Of Control Rats Cv: Central Veinmentioning

confidence: 99%

Furan-induced hepatotoxic and hematologic changes in diabetic rats: the protective role of lycopene

Baş

Pandır

Kalender

2016

Archives of Industrial Hygiene and Toxicology

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“…Typically, HepG2 cells were transfected with vectors containing human CAT cDNA, which had a mitochondrial leader sequence to make comparison of the effectiveness of cytosolic or mitochondrial CAT overexpression for protection against oxidant‐induced injury (Bai and others ). HepG2 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) contained heat‐inactivated FBS (10 mL FBS/100 mL DMEM) and the following antibiotics of penicillin, gentamicin, and streptomycin (Martin and others ; Ham and others ).…”

Section: Evaluation Of Antioxidant Capacity In Cultured Cellsmentioning

confidence: 99%

Evaluation of the Cellular and Animal Models for the Study of Antioxidant Activity: A Review

Zhang

Yin

Huang

et al. 2017

Journal of Food Science

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The mechanisms of antioxidant activities of phytochemicals are highly complex, so various methods to study them have been developed. However, the diverse available methods show inconsistent results. Different stressors, cell models, and animal models have been used to evaluate the antioxidant properties of phytochemicals. However, the literature still lacks a summary of the effects of different stressors, cell models, and animal models on the evaluation of antioxidant activities. Therefore, the mechanisms of action of different oxidative stimuli and the characteristics of the available cell models and animal models are summarized in this review.

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“…USM was prepared from rice bran by saponification; the composition of USM has been described previously (Ham et al 2015a). USM contained 1,635 mg of tocopherols and tocotrienols, 5,514 mg of policosanols, 36,425 mg of phytosterols, and 2,968 mg of oryzanols per 100 g of USM.…”

Section: Preparation Of Usm From Rice Branmentioning

confidence: 99%

“…These health benefits of rice bran have been attributed to the presence of unsaponifiable compounds . We have previously reported that rice bran USM has significant levels of phytochemicals, including tocopherols, tocotrienols, policosanols, phytosterols and oryzanols (Ham et al 2015a). We have previously reported that rice bran USM has significant levels of phytochemicals, including tocopherols, tocotrienols, policosanols, phytosterols and oryzanols (Ham et al 2015a).…”

Section: Introductionmentioning

confidence: 99%

Unsaponifiable Matter from Rice Bran Attenuates High Glucose-Induced Lipid Accumulation by Activating AMPK in HepG2 Cells

Ham

Woo

Lee

et al. 2016

Journal of Food Biochemistry

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Rice bran, a major by‐product of rice milling, has a variety of health benefits, including antioxidant, anti‐inflammatory, and cholesterol‐lowering effects. However, the effects of rice bran unsaponifiable matter (USM) on hepatic lipid metabolism remain unclear. In this study, the effects of rice bran USM in the prevention of high glucose‐induced lipid accumulation and its putative mechanism in HepG2 cells were investigated. USM significantly inhibited high glucose‐induced lipid accumulation and suppressed fatty acid synthase and sterol regulatory element‐binding protein‐1c expression in HepG2 cells. It also increased the phosphorylation of AMP‐activated protein kinase (AMPK) and acetyl coenzyme A carboxylase. Moreover, a specific inhibitor of AMPK attenuated the effects of USM on lipid accumulation. These results demonstrate that rice bran USM prevents hepatic lipid accumulation via an AMPK‐dependent signaling pathway and provides a promising approach for novel lipid‐lowering therapies. Practical Application Rice bran is known to be rich in phytosterols, tocopherols, tocotrienols, and γ‐oryzanol, all of which have health benefits. In recent years, the recognition of the health benefits of rice bran has resulted in its increased use in a range of food products, which far exceeds its usage as a dietary supplement. This study provides biological evidence that supports the use of rice bran unsaponifiable matter in the prevention of hepatic lipid accumulation.

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Protective effects of unsaponifiable matter from rice bran on oxidative damage by modulating antioxidant enzyme activities in HepG2 cells

Cited by 30 publications

References 49 publications

Furan-induced hepatotoxic and hematologic changes in diabetic rats: the protective role of lycopene

Furan-induced hepatotoxic and hematologic changes in diabetic rats: the protective role of lycopene

Evaluation of the Cellular and Animal Models for the Study of Antioxidant Activity: A Review

Unsaponifiable Matter from Rice Bran Attenuates High Glucose-Induced Lipid Accumulation by Activating AMPK in HepG2 Cells

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