Protective effects of tripterygium glycoside-loaded solid lipid nanoparticles on male reproductive toxicity in rats

Abstract

The protective effects of tripterygium glycoside-loaded solid lipid nanoparticles (TG-SLNs) on male reproductive toxicity were investigated in rats. Thirty-six male Sprague-Dawley rats were randomly divided into three groups of 12: control group, tripterygium glycoside (TG) group, and TG-SLN group. After the animals had been orally administered with the substances for 28 consecutive days, their sperm count and sperm motility, organ coefficients, serum testosterone levels, testicular ultrastructure, and reproductive ability were observed. The results showed that the sperm motility rate in the TG group was only 3%, whereas the rates in the TG-SLN and control groups were 33% and 71%, respectively. Compared with those in the control group, the motion counts of path velocity, track speed, progressive velocity, straightness, linearity, beat cross frequency, amplitude of lateral head displacement, and sperm concentrations in the TG-SLN group were not significantly different while those in the TG group significantly decreased (p < 0.01). TG-SLNs did not cause testicular atrophy and instead maintained normal serum testosterone levels. The effect of TG-SLNs on the testicular ultrastructure was very evident; the morphologies of Sertoli, spermatogonial, mitochondrial, and sperm cells were normal. In terms of reproductive ability, one rat (17%) from the TG-SLN group and five rats (83%) from the control group became pregnant, whereas none of the rats from the TG group became pregnant. These data indicate that TG-SLNs have potentially protective effects on male reproductive toxicity in rats.

Impact of Moriamin Forte on Testicular and Epididymal Damage in Rats with Oligoasthenospermia

“…In the present experiment, GTW did not affect either of these parameters; in contrast, testicular index values decreased significantly, suggesting that GTW may cause testicular damage. This finding is similar to that previously reported by Mei et al in a study that involved 28 days of continuous gavage of GTW at the dose of 30 mg (kg bw) −1 [ 36 ]. In MF treatment groups, testicular index values increased, suggesting that MF may reverse testicular damage caused by GTW.…”

“…is finding is similar to that previously reported by Mei et al in a study that involved 28 days of continuous gavage of GTW at the dose of 30 mg (kg bw) −1 [36]. In MF treatment groups, testicular index values increased, suggesting that MF may reverse testicular damage caused by GTW.…”

Case report: A rare case of death due to end-stage renal disease caused by Tripterygium wilfordii-induced myelosuppression

“…Wang et al attempted a transdermal microemulsion drug delivery system for T. wilfordii Hook f. to ameliorate its toxic effects on the male reproductive system ( 18 ). Xue et al reported the protective effects of Tripterygium glycoside-loaded solid lipid nanoparticles against toxicity to the male reproductive system ( 19 ). However, these drug delivery methods neither demonstrate protection of the kidney or hematopoietic system nor are widely used for now.…”

Effects of progesterone nanoparticles on the sperm capacitation and acrosome reaction in asthenozoospermia men

“…Given that sperm motility requires energy and energy supply to the body cells, especially sperms, it is the responsibility of mitochondria, and the role of mitochondria is very important for sperm motility and in fact sperm capacity (Xue et al, 2011). Therefore, any factor that can increase mitochondrial activity in energy production, especially oxidative phosphorylation, oxygen consumption and cell respiration, and finally ATP production has been able to increase sperm capacity (Asghar et al, 2016).…”

“…SLNs are nanoparticles that are synthesized from solid lipids with the diameter of particles of about 50-100 nm (Xue et al, 2011). In recent decades, SLNs are expanded carriers that have been used in kinds of emulsions, liposomes and polymeric nanoparticles (Abd-Allah et al, 2017).…”