Protective Effects of Total Flavonoids fromLitsea coreanaon Alcoholic Fatty Liver in Rats Associated with Down-Regulation Adipose Differentiation-Related Protein Expression

Abstract

Alcoholic fatty liver (AFL) is a reversible condition, but it can potentiate the development of alcoholic hepatitis and even cirrhosis by increasing oxidant generation, which is one of the key pathogenic factors and could result in alcoholic liver disease (ALD). Total flavonoids from Litsea coreana (TFLC), an active component extracted from Litsea coreana leve, have been shown to have therapeutic effects on hyperlipidemia. The present study was to evaluate the protective effects of TFLC on alcoholic fatty liver (AFL) in rats, and investigate the potential mechanism. An AFL model in rats was established by intaking different doses of alcohol (concentration from 5% to 40%) over 12 weeks. Serum levels of TG, TC, LDL-C, HDL-C, TNF-α, insulin, and glucose were measured, histopathologic changes were determined, and expression of adipose differentiation-related protein (ADRP) in the liver were evaluated by Western blotting and immunohistochemistry, respectively. The results showed that treatment with TFLC resulted in decreased serum levels of TG, TC, LDL-C, TNF-α, glucose and insulin, as well as improved liver index. Morphological evaluation revealed rats in model group developed a severe steatosis, but the severities of liver steatosis were effectively ameliorated in TFLC (200 and 400 mg/kg) treated groups. Expression of hepatic ADRP were increased in model group, and suppressed in TFLC treated groups. These results suggest that TFLC had a protective effect on AFL rats; the mechanism may be involved in regulation serum lipid profiles via down-regulation of hepatic expression of ADRP in AFL rats.

Grape-Leaf Extract Attenuates Alcohol-Induced Liver Injury via Interference with NF-κB Signaling Pathway

“…In the current study, the identified compounds in GLE belong to different classes of natural products, e.g., anthocyanins, phenolic acids and derivatives, flavonoids, stilbenoids, and organic acids. Flavonoids were previously reported to exert a protective effect against alcohol-induced liver damage [57][58][59]. Anthocyanins, as a subclass of flavonoids, were previously found to play a role in the prevention of oxidative damage in living systems.…”

A review on phytochemical and pharmacological properties of Litsea coreana

“…Moreover, TFLC showed a protective effect on alcoholic fatty liver in rats. In an alcoholic fatty liver rat model, treatment with 200 mg/kg TFLC reduced the serum levels of triglycerides, total cholesterol, low-density lipoprotein (LDL)-cholesterol, TNF-a, glucose and insulin, and down-regulated the expression of hepatic adipose differentiation-related protein (Hu et al 2012). TFLC (100 mg/L) also reduced the activities of ALT and AST and the level of triglycerides in steatotic hepatocytes, and decreased the mRNA expressions of adipose differentiationrelated protein and peroxisome proliferator-activated receptor c (PPARc) (Hu et al 2013).…”

Prevention and treatment effect of total flavonoids in Stellera chamaejasme L. on nonalcoholic fatty liver in rats

“…Among these, the total flavonoids in Stellera chamaejasme L. (TFSC) and its derivatives with its unique physical and properties has been research hotspot of the world since 1980s, and has made big progress in medicine, food and chemical industry, environmental protection, agriculture and many other fields [ 19 , 20 ]. Recent studies have shown that treatment with flavonoids also benefits patients with NASH and NAFLD [ 21 ], However, the mechanisms responsible for lipid-lowering actions of total flavonoids in Stellera chamaejasme L. (TFSC) on liver remains unclear. In this study, we explored the efficacy of the different doses of TFSC supplements treatment on the HFD-induced hepatic steatosis and hyperlipidemia in rats.…”