Protective Effects of Total Flavones of <i>Elaeagnus rhamnoides</i> (L.) A. Nelson against Vascular Endothelial Injury in Blood Stasis Model Rats

Wei, Zihao; Zuo, Fang; Wang, Wenqian; Wang, Li; Tong, Dong; Zeng, Yong; Wang, Ping; Meng, Xianli; Zhang, Yi

doi:10.1155/2017/8142562

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…The method is different from the freezing method, which is a local and mild cold stress induction method. The cold stress model has been cited by many researchers and belongs to the recognized method of cold coagulation stasis syndrome model in traditional Chinese medicine [27][28][29]. However, adrenaline, instead of subcutaneous injection of epinephrine, is the cause of blood stasis; thus, we used an ice water bath as a direct cold stimulus to induce reproductive stress in rats and examine whether it could affect body circulation and local reproductive organ structure and function [30].…”

Section: Discussionmentioning

confidence: 99%

Effect of cold stress on ovarian & uterine microcirculation in rats and the role of endothelin system

Wang

Xiao

Fang

et al. 2020

Reprod Biol Endocrinol

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Background: Cold, an environmental factor, induces many reproductive diseases. It is known that endothelin (ET) is a potent vasoconstrictor, and cold stress can increase the expression of ET and its receptors. The cold stress rat model was developed to examine two parameters: (1) the effects of cold stress on ovarian and uterine morphology, function, and microvascular circulation and (2) possible mechanisms of ET and its receptors involved in cold stress-induced menstruation disorders. Methods: The rat cold stress model was prepared with an ice water bath. The estrous cycle was observed by methylene blue and hematoxylin and eosin (H&E) staining. Serum estradiol 2 (E 2), testosterone (T), progesterone (P) were detected by radioimmunoassay. Hemorheology indices were measured. The real-time blood flow of auricle and uterine surfaces was measured. Expressions of CD34 and α-SMA in ovarian and uterine tissues were detected by immunohistochemistry. ET-1 contents in serum were tested, and expressions of ET-receptor types A and B (ET-AR and ET-BR) in ovarian tissues were detected via Western blotting. Results: Cold stress extended the estrous cycle, thereby causing reproductive hormone disorder, imbalance of local endothelin/nitric oxide expression, and microcirculation disturbance. Cold-stress led to up-regulation of ET-AR expression and protein and down-regulation of ET-BR expression in rats. Conclusions: This study suggests that the reason for cold stress-induced dysfunction in reproductive organs may be closely related to the imbalance of ET-1 and its receptor expressions, leading to microvascular circulation disorders in local tissues.

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Section: Discussionmentioning

confidence: 99%

Effect of cold stress on ovarian & uterine microcirculation in rats and the role of endothelin system

Wang

Xiao

Fang

et al. 2020

Reprod Biol Endocrinol

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“…Total flavonoids from TFSB was prepared using a published method (Wei et al, ). In brief, sea buckthorn fruit was firstly dried, crushed, and degreased.…”

Section: Methodsmentioning

confidence: 99%

Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke‐induced airway inflammation

Ren

et al. 2019

Phytotherapy Research

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The total flavonoids from sea buckthorn (TFSB) exhibit a potent anti-inflammatory activity; however, the effect of TFSB on respiratory inflammatory disease is not fully known. The present study evaluated the potential of TFSB to prevent airway inflammation and the underlying mechanism. The results showed that TFSB remarkably inhibited lipopolysaccharide/cigarette smoke extract (LPS/CSE)-induced expression of IL-1β, IL-6, CXCL1, and MUC5AC at both mRNA and protein levels in HBE16 bronchial epithelial cells. TFSB also decreased the production of PGE 2 through inhibition the expression of COX2 in LPS/CSE-stimulated HBE16 cells. Furthermore, bronchoalveolar fluid and histological analyses revealed that LPS/cigarette smoke exposure-induced elevated cell numbers of neutrophils and macrophages in bronchoalveolar fluid, inflammatory cell infiltration, and airway remodeling were remarkably attenuated by TFSB in mice. Immunohistochemical results also confirmed that TFSB decreased the expression of IL-1β, IL-6, COX2, CXCL1, and MUC5AC in LPS/CSexposed mice. Mechanistically, TFSB blocked LPS/CSE-induced activation of ERK, Akt, and PKCα. Molecular docking further confirmed that the main components in TFSB including quercetin and isorhamnetin showed potent binding affinities to MAPK1 and PIK3CG, two upstream kinases of ERK and Akt, respectively. In summary, TFSB exerts a potent protective effect against LPS/CS-induced airway inflammation through inhibition of ERK, PI3K/Akt, and PKCα pathways, suggesting that TFSB may be a novel therapeutic agent for respiratory diseases. KEYWORDS chronic bronchitis, ERK, PI3K/Akt, and PKC pathways, lipopolysaccharide/cigarette smoke, sea buckthorn, total flavonoids

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“…The flavonoid preparation caused a reduction of whole-blood viscosity, lengthening of PT and APTT at the doses of 0.24 and 0.48 g kg −1 b.w., and decreasing of the level of fibrinogen. The reduction of levels of thromboxane B2, 6-keto-prostaglandin F1α, von Willebrand factor (vWF),and thrombomodulin was also observed (Wei et al 2017 ).…”

Section: Pharmacologymentioning

confidence: 97%

Phytochemistry and pharmacology of sea buckthorn (Elaeagnus rhamnoides; syn. Hippophae rhamnoides): progress from 2010 to 2021

Żuchowski

2022

Phytochem Rev

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Sea buckthorn (Elaeagnus rhamnoides; syn. Hippophae rhamnoides) is a thorny shrub or a small tree belonging to the Elaeagnaceae family, native to Eurasia. Sea buckthorn fruit is rich in vitamins and minerals, oils from the seeds and fruit flesh find use in medicine and the cosmetic industry or as nutraceutical supplements. Fruit, leaves and other parts of buckthorn have been used in traditional medicine, especially in China, Tibet, Mongolia, and Central Asia countries, and are a rich source of many bioactive substances. Due to its health-promoting and medicinal properties, the plant has been extensively investigated for several decades, and its phytochemical composition and pharmacological properties are well characterized. The years 2010–2021 brought significant progress in phytochemical research on sea buckthorn. Dozens of new compounds, mainly phenolics, were isolated from this plant. Numerous pharmacological studies were also performed, investigating diverse aspects of the biological activity of different extracts and natural products from sea buckthorn. This review focuses on the progress in research on sea buckthorn specialized metabolites made in this period. Pharmacological studies on sea buckthorn are also discussed. In addition, biosynthetic pathways of the main groups of these compounds have been shortly described. Graphical abstract

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Protective Effects of Total Flavones of Elaeagnus rhamnoides (L.) A. Nelson against Vascular Endothelial Injury in Blood Stasis Model Rats

Cited by 5 publications

References 31 publications

Effect of cold stress on ovarian & uterine microcirculation in rats and the role of endothelin system

Effect of cold stress on ovarian & uterine microcirculation in rats and the role of endothelin system

Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke‐induced airway inflammation

Phytochemistry and pharmacology of sea buckthorn (Elaeagnus rhamnoides; syn. Hippophae rhamnoides): progress from 2010 to 2021

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