Protective effects of tocotrienols against lipid-induced nephropathy in experimental type-2 diabetic rats by modulation in TGF-β expression

Siddiqui, Shahla; Ahsan, Haseeb; Khan, Mohammad Rashid; Siddiqui, Waseem Ahmad

doi:10.1016/j.taap.2013.09.004

Cited by 52 publications

(60 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this line, there are previous documented reports which signify that HFD-STZ-induced diabetic rats exhibit pathological features including hyperglycemia, insulin resistance and oxidative stress [47,48]. Oral administration of RA to the diabetic rats improves ISI 0-120 and protects pancreatic beta cell mass thereby increasing insulin levels and reduces hyperglycemia via its ability to improve insulin sensitivity; the effect of which was similar to that observed on metformin administration.…”

Section: Discussionsupporting

confidence: 65%

Rosmarinic acid modulates the antioxidant status and protects pancreatic tissues from glucolipotoxicity mediated oxidative stress in high-fat diet: streptozotocin-induced diabetic rats.

Govindaraj

Pillai

2015

Mol Cell Biochem

View full text Add to dashboard Cite

Persistent hyperglycemia and elevated levels of free fatty acids (FFA) contribute to oxidative stress, a proximate cause for the onset and progression of diabetes and its complications. The present study was hypothesized to evaluate the anti-diabetic potential of Rosmarinic acid (RA) during high-fat diet (HFD)-streptozotocin (STZ)-induced type 2 Diabetes (T2D) in wistar albino rats. Oral administration of RA (100 mg/kg b.w) significantly (p < 0.05) increased the insulin sensitivity index (ISI0,120), while the levels of blood glucose, HbA1c, advanced glycation end products (AGE), TNF-α, IL-1β, IL 6, NO, p-JNK, P38 MAPK and NF-κB were significantly reduced, with a concomitant elevation in the plasma insulin levels in diabetic rats. Furthermore, RA treatment significantly (p < 0.05) reduced the levels of triglycerides, FFA and cholesterol in serum, and reduced the levels of lipid peroxides, AOPP's and protein carbonyls in the plasma and pancreas of diabetic rats. The diminished activities of pancreatic superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) and the decreased levels of plasma ceruloplasmin, vitamin C, vitamin E and reduced glutathione (GSH) in diabetic rats were also significantly (p < 0.05) recovered upon RA treatment denoting its antioxidant potential which was confirmed by Nrf-2, hemeoxyenase (HO-1) levels. Histological, ultrastructural and immunohistochemical data demonstrate that oral administration of RA protects pancreatic β-cells from oxidative niche in HFD-STZ-induced experimental diabetes. Our findings suggest that the oral treatment with RA alleviates pancreatic β-cell dysfunction and glucolipotoxicity-mediated oxidative stress during HFD-STZ-induced T2DM, perhaps through its antioxidant potential.

show abstract

Section: Discussionsupporting

confidence: 65%

Rosmarinic acid modulates the antioxidant status and protects pancreatic tissues from glucolipotoxicity mediated oxidative stress in high-fat diet: streptozotocin-induced diabetic rats.

Govindaraj

Pillai

2015

Mol Cell Biochem

View full text Add to dashboard Cite

show abstract

“…Following SCI, the lipid peroxidation process can inhibit the activity of Ca 2+ -ATPase and interfere with the movement of Ca 2+ out of the cell membrane, causing intracellular Ca 2+ overload and ionic imbalance (4). The present results suggest that the neuroprotective effect of tocotrienol reduced the serum MDA level and increased the serum SOD, CAT and GSH-PX levels in SCI rats (21). Siddiqui et al showed that protective effects of tocotrienol may protect against nephropathy in experimental type-2 diabetic rats by suppression of oxidative stress (21).…”

Section: Discussionsupporting

confidence: 52%

“…Post-SCI oxidative stress damages the ion steady state inside and outside the mitochondrial membrane, Ca 2+ accumulates inside the mitochondria, causing mitochondrial damage and aerobic energy metabolic disorders and inhibiting the synthesis of ATP (4). Following SCI, excessive activity of free radicals may be applied to the postsynaptic neuron, activating nearby astrocytes and microglia, causing ionic imbalance at the neuronal cell membrane (21). In addition, Ca 2+ -ATPase of the plasma Figure 6.…”

Section: Discussionmentioning

confidence: 99%

“…The present results indicate that treatment with tocotrienols significantly inhibited TGF-β protein expression in SCI rats. Siddiqui et al (21) reported that tocotrienol mitigates the damage of lipid-induced nephropathy through suppression of TGF-β and collagen type IV. Fibronectin is a multifunctional glycoprotein found in the extracellular matrix and plasma (35).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Tocotrienol alleviates inflammation and oxidative stress in a rat model of spinal cord injury via suppression of transforming growth factor-β

Xun

Mamat

Guo

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. In recent years accumulating evidence has indicated that tocotrienol exhibits an oxidation resistance function, decreased cholesterol function, inhibits cancer function and has unique physiological functions, including anti-inflammatory, anti-apoptotic and anti-oxidative properties. The present study investigated the effect of tocotrienols on spinal cord injury (SCI) by evaluating oxidative stress, inflammation and inducible nitric oxide synthase (iNOS) in rats. A rat model of SCI was induced by operation. SCI rats were treated with 120 mg/kg/day tocotrienol once a day for eight consecutive weeks. Functional recovery following SCI was measured by using the Basso Beattie Bresnahan (BBB) locomotor rating scale. Then the volume of spinal cord contusions was measured following induction of SCI in the rats. In SCI rats, serum malondialdehyde, superoxide dismutase, catalase, glutathione peroxidase, nuclear factor-κB p65 unit, tumor necrosis factor-α, interleukin (IL)-1β and IL-6 levels were analyzed using respective commercial immunoassay kits. Firstly, iNOS, transforming growth factor (TGF)-β, collagen type IV and fibronectin protein expression levels, in addition to iNOS activity and plasma nitric oxide (NO) production in SCI rats was analyzed using western blot analysis, commercial kits and Griess reagent, respectively. Tocotrienol treatment elevated BBB scores and contused volume in the SCI rats. Tocotrienol protected against SCI with reduced oxidative stress and inflammation, and inhibited iNOS protein expression iNOS activity and plasma NO production in rats. In addition, treatment with tocotrienols suppressed TGF-β, collagen type IV and fibronectin protein expression levels in SCI rats. These results suggest that tocotrienols protect SCI, and suppress oxidative stress, inflammation and iNOS in this model of SCI through TGF-β, collagen type IV and fibronectin signaling pathways.

show abstract

“…Tocopherols and tocotrienols are vitamin E isomers which are present in high amount (800-1270 mg kg -1 ) in PO (Puah et al, 2007). Palm vitamin E possesses unique properties such as antioxidant (Cerecetto and López, 2007), cardioprotective (Heng et al, 2013), neuroprotective (Mangialasche et al, 2010), anti-cancer and cancer suppression (Wong and Radhakrishnan, 2012;Nesaretnam et al, 1998), nephroprotective (Siddiqui et al, 2010), anti-diabetic (Siddiqui et al, 2013), gastroprotective (Azlina et al, 2005), hepatoprotective (Ngah et al, 1991) and neurologic and brain development effects (Traber, 2014).…”

Section: Dietary Supplementmentioning

confidence: 99%

Palm Oil and Palm Kernel Oil: Versatile Ingredients for Food Applications

Dian¹

2018

JOPR

View full text Add to dashboard Cite

show abstract

Protective effects of tocotrienols against lipid-induced nephropathy in experimental type-2 diabetic rats by modulation in TGF-β expression

Cited by 52 publications

References 70 publications

Rosmarinic acid modulates the antioxidant status and protects pancreatic tissues from glucolipotoxicity mediated oxidative stress in high-fat diet: streptozotocin-induced diabetic rats.

Rosmarinic acid modulates the antioxidant status and protects pancreatic tissues from glucolipotoxicity mediated oxidative stress in high-fat diet: streptozotocin-induced diabetic rats.

Tocotrienol alleviates inflammation and oxidative stress in a rat model of spinal cord injury via suppression of transforming growth factor-β

Palm Oil and Palm Kernel Oil: Versatile Ingredients for Food Applications

Contact Info

Product

Resources

About