Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress

Li, Yinfeng; Liu, Hua; Jiang, Guilan; Yu, Zhihai; Huang, Mingzheng; Zou, Shiping; Guan, Tianbing; Yan, Zhao; Liu, Xiaozhu

doi:10.3389/fmicb.2022.1057284

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…The resulting paired-end RNA-seq sequencing library was then sequenced using the Illumina HiSeq xten/NovaSeq 6000 sequencer (Illumina, San Diego, CA, USA). We conducted the identification of DEGs, classification analysis of DEGs, and KEGG enrichment analysis of DEGs using the Majorbio Cloud Platform ( https://cloud.majorbio.com ) according to the method of Li and colleagues [ 47 ].…”

Section: Methodsmentioning

confidence: 99%

Contribution of trehalose to ethanol stress tolerance of Wickerhamomyces anomalus

Li,

Jiang,

Long

et al. 2023

BMC Microbiol

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Background The ascomycetous heterothallic yeast Wickerhamomyces anomalus (WA) has received considerable attention and has been widely reported in the winemaking industry for its distinctive physiological traits and metabolic attributes. An increased concentration of ethanol during ethanol fermentation, however, causes ethanol stress (ES) on the yeast cells. Trehalose has been implicated in improving survival under various stress conditions in microorganisms. Herein, we determined the effects of trehalose supplementation on the survival, differentially expressed genes (DEGs), cellular morphology, and oxidative stress tolerance of WA in response to ES. Results The results indicated that trehalose improved the survival and anomalous surface and ultrastructural morphology of WA. Additionally, trehalose improved redox homeostasis by reducing the levels of reactive oxygen species (ROS) and inducing the activities of antioxidant enzymes. In addition, DEGs affected by the application of trehalose were enriched in these categories including in gene expression, protein synthesis, energy metabolism, and cell cycle pathways. Additionally, trehalose increased the content of intracellular malondialdehyde (MDA) and adenosine triphosphate. Conclusions These results reveal the protective role of trehalose in ES mitigation and strengthen the possible uses of WA in the wine fermentation sector.

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Section: Methodsmentioning

confidence: 99%

Contribution of trehalose to ethanol stress tolerance of Wickerhamomyces anomalus

Li,

Jiang,

Long

et al. 2023

BMC Microbiol

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“…Moreover, ethanol also regulates the expression of numerous genes related to energy metabolism, lipid metabolism, amino acid metabolism and transportation of nutrients (Auesukaree et al, 2017). Additionally, ethanol could induce the accumulation of multiple endogenous protective substances, like trehalose (Wang et al, 2014), proline (Takagi et al, 2016) and thiamine (Li et al, 2022a).…”

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confidence: 99%

Exogenous trehalose application promotes survival by alleviating oxidative stress and affecting transcriptome in ethanol-stressed Wickerhamomyces anomalus

Jiang

Liu

et al. 2023

BFJ

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PurposeIncreased ethanol accumulation during ethanol fermentation generates stress in yeast cells, which finally reduces the fermentation performance and efficiency. Trehalose, a potential stress protectant, has been reported to regulate the response of yeast to diverse environmental stresses. This study aimed to explore how exogenous trehalose application affects the survival, transcriptome and antioxidant enzymes of Wickerhamomyces anomalus grown under ethanol stress conditions.Design/methodology/approachExogenous trehalose was applied to the growth condition of W. anomalus, and optical densitometric method was used to detect contents of intracellular trehalose and MDA and activities of CAT and SOD. The survival was evaluated using spot analysis. Differentially expressed genes (DEGs) were identified through transcriptomics analysis.FindingsThe results showed that ethanol stress induced the accumulation of intracellular trehalose, with further exogenous trehalose application improving the survival and alleviating oxidative stress in ethanol-stressed W. anomalus. Transcriptomic results showed that trehalose has pleiotropic regulating effects on ethanol-stressed W. anomalus since most DEGs annotated to energy metabolism, amino acid metabolism, translation, folding, sorting and transport were affected post trehalose addition. Therefore, it is found that trehalose protected W. anomalus against ethanol stress, and these findings provide interesting insights into the mechanistic role of trehalose in improving ethanol stress tolerance of W. anomalus.Originality/value(1) Protective effect of exogenous trehalose addition on the survival of ethanol-stressed W. anomalus was proved. (2) Exogenous trehalose addition could partly alleviate oxidative stress induced by ethanol stress and affect transcriptome in W. anomalus.

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Transcriptomics to evaluate the influence mechanisms of ethanol on the ester production of Wickerhamomyces anomalus with the induction of lactic acid

Cai,

Wan,

Chen

et al. 2024

Food Microbiology

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Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress

Cited by 5 publications

References 29 publications

Contribution of trehalose to ethanol stress tolerance of Wickerhamomyces anomalus

Contribution of trehalose to ethanol stress tolerance of Wickerhamomyces anomalus

Exogenous trehalose application promotes survival by alleviating oxidative stress and affecting transcriptome in ethanol-stressed Wickerhamomyces anomalus

Transcriptomics to evaluate the influence mechanisms of ethanol on the ester production of Wickerhamomyces anomalus with the induction of lactic acid

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