Protective Effects of the Potent Na/H Exchange Inhibitor Methylisobutyl Amiloride Against Post-ischemic Contractile Dysfunction in Rat and Guinea-pig Hearts

Ultrastructural Evidence of the Protective Effect of Na+/H+ Exchange Inhibition on the in vitro Damage Induced by Ischaemia Reperfusion in the Interventricular Septum of the Rabbit Heart

“…ischaemia after cardioplegic arrest of the whole heart (Koike et al 1996), and decreases the magnitude of cell death in the isolated arterially perfused rabbit papillary muscles when evaluated by the trypan blue uptake method (Kaplan et al 1995). There are several publications that this drug also exhibits myocardial protective effects on models of ischaemia and reperfusion in other animal species such as rat (Meng & Pierce 1990), guinea-pig (Moffat & Karmazyn 1993) and pig (Klein et al 1995). We found the maximal cardioprotective effects at a concentration of 10 mM, which has been proven as effective in the blockade of the Na π /H π exchanger in the rabbit heart without affecting cellular energy (Koike et al 1996).…”

Role of endothelin‐1, sodium hydrogen exchanger‐1 and mitogen activated protein kinase (MAPK) activation in glucose‐induced cardiomyocyte hypertrophy

“…For ET peptide treatment, cells were exposed to 5 nmol/L ET-1 (Peninsula Laboratories, Belmont, CA) [23]. The specificity and concentrations of these agents have been previously established [23][24][25]. All experiments were carried out after 2-4 days of incubation unless otherwise indicated.…”

“…L-glucose was used as a control. In all experiments, dual ET-receptor antagonist bosentan (courtesy of Dr M Clozel, Acetelion ltd, Allschwill, Switzerland) [23], NHE inhibitor cariporide (Courtesy of Dr M Karmazyn) [24], and mitogen activated protein kinase (MAPK) inhibitor U0126 (Promega Corporation, Pittsburgh, PA) [25], were used at 10 µmol/L. Protein kinase C (PKC) inhibitor chelerythrine was used at 1 µmol/L [25].…”

Na+/H+‐Exchange Inhibition by Cariporide (Hoe 642): A New Principle in Cardiovascular Medicine

“…Amiloride derivatives, administered prior to regional ischemia, prevent ischemia-associated arrhythmias and reduce ischemic damage (22,33,35,43,49). However, these amiloride derivatives are not specific for the cardiac Na + /H + -exchanger.…”