Ultrastructural Evidence of the Protective Effect of Na+/H+ Exchange Inhibition on the in vitro Damage Induced by Ischaemia Reperfusion in the Interventricular Septum of the Rabbit Heart

Salinas, Pedro; Gil-Loyzaga, Pablo; Barrigón, Santos

doi:10.1034/j.1600-0773.2000.pto860505.x

Cited by 1 publication

(2 citation statements)

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“…In accordance with previous reports, 4,5,23 the addition of DMA appeared to be associated with the preservation of cell viability and plasma membrane integrity. Because it is unlikely that lethally injured myocytes would be able to retain [ 3 H]‐2‐DG, increased retention of [ 3 H]‐2‐DG in the ischaemic–reperfused DMA‐treated hearts was evidence that DMA directly or indirectly provided protection against plasma membrane injuries.…”

Section: Discussionsupporting

confidence: 92%

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Protection of Ischaemic‐reperfused Rat Heart by Dimethylamiloride Is Associated With Inhibition of Mitochondrial Permeability Transition

Prendes

Torresín²,

González³

et al. 2007

Clin Exp Pharma Physio

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1. The aim of the present study was to assess whether protection afforded by the Na(+)/H(+) exchanger blocker dimethylamiloride (DMA) is associated with inhibition of mitochondrial permeability transition (MPT). The effects of DMA were compared with those of cyclosporine (Cs) A, an inhibitor of MPT. 2. Rat hearts were Langendorff perfused with Krebs'-bicarbonate medium containing 10 mmol/L glucose and were subjected to 25 min no-flow global ischaemia and 30 min reperfusion in the presence or absence of 10 micromol/L DMA or 0.2 micromol/L CsA. Cell viability was measured using tetrazolium stain. The MPT was determined by loading hearts with 2-deoxy-[(3)H]-glucose (2DG), which enters mitochondria only during MPT. Total heart 2DG content as an estimation of the extent of tissue damage was also measured. To assess whether DMA has any direct effect on glycolysis, a cell-free heart extract containing all the glycolytic enzymes was used. 3. Dimethylamiloride improved functional recovery (rate-pressure product) from 24 +/- 7 to 68 +/- 11% (P < 0.01) at reperfusion end, attenuated the increase in left ventricular end-diastolic pressure (from 29 +/- 7 to 6 +/- 3% 10 min after reperfusion onset; P < 0.01), improved cell viability (from 21.2 +/- 6.6 to 69.6 +/- 7.1% at reperfusion end; P < 0.05) and lessened lactate accumulation at the end of ischaemia (119 +/- 15 vs 163 +/- 14 micromol/g dry weight; P < 0.05). Dimethylamiloride limited MPT: 2DG mitochondrial entrapment, being 33.1 +/- 14.2 and 96.3 +/- 14.0 at reperfusion end in the treated and control hearts, respectively (P < 0.05), and concomitantly raised total 2DG content (51.3 +/- 4.4 vs 86.8 +/- 1.7 x 10(3) d.p.m./g wet weight in control and treated groups, respectively; P < 0.05). Cyclosporine A improved functional recovery and attenuated the amplitude of ventricular diastolic pressure in ischaemic-reperfused hearts. It also reduced mitochondrial entrapment (67.3 +/- 7.7%; P < 0.05 vs control) and increased total cell 2DG content (162.3 +/- 1.3 x 10(3) d.p.m./g wet weight; P < 0.01 vs control) at the end of reperfusion. Dimethylamiloride did not affect glucose consumption and lactate production in the cell-free heart extract. 4. In conclusion, DMA protects against the noxious effects of ischaemia-reperfusion and inhibits MPT, coinciding with present and previous findings concerning the effects of CsA. Dimethylamiloride also diminished lactate accumulation, although it did not exhibit any direct effect on glycolysis. These data suggest that blockade of Na(+)/H(+) exchange by DMA attenuates the extent of MPT in ischaemic-reperfused rat heart.

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Section: Discussionsupporting

confidence: 92%

“…Inhibition of the Na + /H + exchanger (NHE) has been demonstrated to protect against ischaemia–reperfusion injury, enhancing contractile recovery, reducing the incidence of arrhythmias and delaying cell death. However, the mechanisms responsible for these protective effects have yet to be fully understood 1–8 …”

Section: Introductionmentioning

confidence: 99%