PROTECTIVE EFFECTS OF THE GLUCOCORTICOID, BUDESONIDE, ON LUNG ANAPHYLAXIS IN ACTIVELY SENSITIZED GUINEA‐PIGS: INHIBITION OF IgE — BUT NOT OF IgG — MEDIATED ANAPHYLAXIS

Abstract

The effect of glucocorticoid pretreatment on antigen‐induced bronchoconstriction was studied in guinea‐pigs actively sensitized to two different ovalbumin regimens (one producing IgE‐ and IgG‐like antibodies and the other exclusively IgG‐like antibodies). Budesonide (50 mg/kg) and hydrocortisone (50 mg/kg) given as one intraperitoneal injection 15–20 h before the anaphylactic test or as two consecutive intraperitoneal injections 5 and 6 days before, led to a decreased bronchial capacity. In this respect glucocorticoid pretreatment was effective only in guinea‐pigs sensitized to produce both IgE‐like and IgG‐like antibodies. Budesonide pretreatment also reduced the capacity of anaphylactically‐challenged chopped lung tissue to release histamine in guinea‐pigs sensitized to produce both IgE‐ and IgG‐like antibodies. Budesonide pretreatment did not change the levels of circulating IgG1a and IgE‐like homocytotropic antibodies as measured by passive cutaneous anaphylaxis; nor did it affect histamine or methacholine‐induced bronchoconstriction in vivo or the capacity of histamine or methacholine to contract the guinea‐pig isolated trachea preparation or the isoprenaline‐induced relaxation of this preparation. The selective inhibitory effects of budesonide and hydrocortisone on IgE‐mediated but not IgG‐mediated anaphylaxis and the relevance to human atopic disease are discussed.

Changes in bronchial anaphylactic reactivity induced in guinea‐pigs by long‐term treatment with histamine H2‐agents

“…Respiratory measurements These were performed as described in detail previously (Andersson, 1980a;Andersson & Bergstrand, 1981;Andersson & Brattsand, 1982). In brief, pulmonary mechanics, i.e.…”

Corticosteroids in management of anaphylaxis; a systematic review of evidence

“…The animal models used included guinea pigs (62)(63)(64) and mice (48,53,65,66). One other study was conducted using A549 human adenocarcinoma cell line (52).…”

Strain‐dependency of leukotriene C4 generation from isolated lungs of immunized mice

“…'." Macmillan Press Ltd, 1994 Methods Animals and sensitization procedure Male Swiss, Balb/C and CBA/J mice aged 8-weeks, weighing approximately 25-30 g raised at the Pasteur Institute (Paris, France) were actively sensitized by a subcutaneous (s.c.) injection of 0.4 ml 0.9% w/v NaCl (saline) containing 100 gg ovalbumin adsorbed in 1.6 mg Al(OH)3 (Andersson & Brattsand, 1982). Seven days later, the animals received the same dose of ovalbumin with Al(OH)3 and were used 7 days thereafter.…”