Protective Effects of Squid Ink Extract Towards Hemopoietic Injuries Induced by Cyclophosphamine

Abstract

Abstract:To investigate the protective effects of squid ink in chemotherapy, BALB/c mice were used as animal models of injuries induced by cyclophosphamine, a well known chemotherapeutic drug. The mice were randomly divided into five groups with the same number of males and females in each group. At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells. Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05). In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals. The data suggest that squid ink extract can protect the hemopoietic system from chemotherapeutic injury and could be employed to develop cell-protective drugs for use in clinical treatment of tumours.

Therapeutic effect of low molecular weight chitosan containing sepia ink on ethanol-induced gastric ulcer in rats

“…In the present study, we evaluated the therapeutic effect of LMCS on ethanol-induced gastric ulcer in rats, and results manifested that oral administration of LMCS (7.6 mg/kg) has obviously promoting healing effect. Our previous work had illuminated that chitosan and sepia ink has good biocompatibility and regulation effect for gastrointestinal diseases 12 . But the underlying therapeutic mechanism of compound LMCS in gastric ulcer is still unclear.…”

“…Also, sepia ink is a traditional medicine used in the treatment of hemostasis for centuries in China 12 , and some researchers have founded that sepia ink has anti-radiation activity, antioxidant activity, antitumor activity, immunomodulatory activity, procoagulant function, etc 13 . It can improve the functional status of the whole body 14 .…”

Antibacterial Activity of Melanin from Cuttlefish and Squid Ink

“…Tinta cumi-cumi dapat berperan sebagai obat pelindung sel pada pengobatan kanker dengan cara kemoterapi, melalui peningkatan jumlah sel leukosit dan sel nucleat sumsum tulang, yang jumlahnya menurun akibat penggunaan obat pembunuh sel tumor tersebut. Melanin dari tinta cumicumi mempunyai aktivitas anti-tumor dengan menghambat aktivitas plasmin untuk meningkatkan thromboxan dan meningkatkan sistem imun untuk membunuh sel kanker (Zhong et al 2009). Melanin juga berperan sebagai antioksidan (Lei et al 200 7a ), anti-radiasi (Lei et al 2007 b ), dan antirotavirus (Rajaganapathi et al 2007).…”

Mediterranien cuttlefish sepia officinalis squid ink is cytotoxic but does not inhibit Glioblastoma U87 tumor cells proliferation, with high nutritional values of edible viscera

“…Currently the co-products, poorly valued are essentially processed into meal and oil, intended primarily for animal feed [3][4]. Working on capelin fish [5], on the shark [6], these others showed that heads, bones, guts and skins are an important source of proteins and essential amino acids and have a significant nutritional value. Food markets, pharmaceutical or cosmetics could accommodate such as fishery products.…”

“…Some researchers are interested in the development of squid hepatopancreas [7], [8], other in the quality of viscera of cuttlefish Sepia officinalis and its digestive gland [9]. Squid ink (liquid co-product), is also a traditional medicine reamed used often as a treatment for heart disease, gynecology and surgery [6]. It contains alarm indices: uridine, cytidine and uracil [7].…”