Protective effects of selenium on mercury-induced DNA damage in mussel haemocytes

Tran, Damien; Moody, A. John; Fisher, Andrew; Foulkes, Mike E.; Jha, Awadhesh N.

doi:10.1016/j.aquatox.2007.05.009

Cited by 77 publications

(39 citation statements)

References 34 publications

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“…We demonstrated that the mercury chloride does not generate the damage on mtDNA of Drosophila melanogaster in 24 hours treatment. There is some evidence of mercury toxicity on DNA (Chen et al, 2005;Al-azzawie et al, 2013;Costa et al, 1991;Tran et al, 2007;Pereira et al, 2010;Barcelos et al, 2011) but there are few studies on the effect on mtDNA in literature. Wyatt and his team have come to the conclusion that, in their study on C. elegans, both inorganic and organic mercury have damaged the mtDNA (Wyatt et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Civa Klorürün mtDNA'da Akut Etkisi

Mutlu¹,

Yildiz²

2017

Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Mercury is a highly toxic environmental pollutant. Molecular mechanisms of mercury toxicity are assorted. Basically, they block essential functional groups in biomolecules and also displace essential metal ions from them. Mercuric ion is known as one of the strongest thiol-binding agents. Although the oxidative properties of mercury have been studied and accepted the actual process of ROS generation is still unclear. The aim of the current study is to examine the acute effects of mercury chloride exposure on mtDNA damage and mtDNA copy number in Drosophila. Quantitative PCR method was used to measure mtDNA damage. In the mercury chloride application groups, mtDNA damage and mtDNA copy number were slightly greater than the control group but the difference was not statistically significant. We demonstrated that the mercury chloride application does not generate the damage on mtDNA of Drosophila melanogaster in 24 hours treatment.Keywords: Mercury, mtDNA damage, mtDNA copy number, Drosophila Civa Klorürün mtDNA'da Akut Etkisi ÖZ Civa çok zehirli bir çevresel kirleticidir. Civa toksisitesinin moleküler mekanizmaları çeşitlidir. Esasen, biyomolekül-lerdeki temel fonksiyonel grupları bloke eder ve temel metal iyonlarını onlardan uzaklaştırır. Civa iyonu, en güçlü tiol bağlayıcı ajanlardan biri olarak bilinir. Civanın oksidatif özellikleri araştırılmış ve kabul edilmiş olsa da, ROS oluşu-munun gerçek süreci hala belirsizdir. Bu çalışmanın amacı, civa klorür maruziyetinin, Drosophila'daki mtDNA hasarı ve mtDNA kopya sayısı üzerindeki akut etkilerini incelemektir. Çalışmamızda mtDNA hasarını ölçmek için kantitatif PCR yöntemi kullanıldı. Sonuçlara göre, civa klorür uygulama gruplarında mtDNA hasarı ve mtDNA kopya sayısı kontrol grubundan biraz daha fazlaydı, ancak bu fark istatistiksel olarak anlamlı değildi. Buna dayanarak, civa klorür uygulamasının Drosophila melanogaster'in mtDNA'sında, akut hasar oluşturmadığı sonucuna varılmıştır.

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Section: Resultsmentioning

confidence: 99%

Civa Klorürün mtDNA'da Akut Etkisi

Mutlu¹,

Yildiz²

2017

Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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“…[17] In 1997, Yoneda and Kazuo showed that toxicity of mercury can be reduced by coadministration with selenium. [13] Our strategy is to take advantage of the potential protective effect of Se against Hg toxicity [14] and design and synthesize a novel organoselenium fluorescent probe (FSe-1), which is nonfluorescent in aqueous solution but gives a fluorescence turn-on response upon Hg II addition. Unlike conventional chemosensors that operate by binding to Hg 2 + ions, FSe-1, which utilizes a deselenation reaction, features ultrahigh sensitivity and selectivity for mercury over other metal ions and related bioanalytes.…”

Section: IImentioning

confidence: 99%

“…Thus, we report a new route for improving sensitivity and selectivity in the detection of Hg 2 + ions in biological and environmental systems. [12] Selenium is an essential element in vivo [13] and mainly functions through selenoprotein, a vitally important protein in biological defense systems due to its antioxidative [14] and antitoxic actions towards heavy-metal ions. [13] Ebselen (2-phenyl-1,2-benzisoselenazol-3(2H)-one), a known mimetic Abstract: Inspired by the antitoxic function of selenium towards heavymetal ions, we designed an organoselenium fluorescent probe (FSe-1) for mercury.…”

Section: IImentioning

confidence: 99%

Use of Selenium to Detect Mercury in Water and Cells: An Enhancement of the Sensitivity and Specificity of a Seleno Fluorescent Probe

Tang

Ding

et al. 2009

Chemistry A European J

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Seleno fluorescent probe: An organoselenium fluorescent probe (FSe-1) for mercury was designed based on the irreversible deselenation mechanism. FSe-1 exhibits an ultrahigh selectivity and sensitivity for Hg(2+) detection only for reactive selenium atom sites, due the strong affinity between Se and Hg. Furthermore, the new probe has been successfully used for imaging mercury ions in RAW 264.7 cells (a mouse macrophage cell line; see figure).Inspired by the antitoxic function of selenium towards heavy-metal ions, we designed an organoselenium fluorescent probe (FSe-1) for mercury. The reaction of FSe-1 and Hg(2+) is an irreversible deselenation mechanism based on the selenophilic character of mercury. FSe-1 exhibits an ultrahigh selectivity and sensitivity for Hg(2+) detection only for reactive selenium atom sites due to the strong affinity between Se and Hg. The experimental results proved that FSe-1 was selective for Hg(2+) ions over other relevant metal ions and bioanalytes, and also showed an enhancement in sensitivity of up to 1.0 nM, which is lower than the current Environmental Protection Agency standard for drinking water. Furthermore, the new probe has been successfully applied to the imaging of mercury ions in RAW 264.7 cells (a mouse macrophage cell line) with high sensitivity and selectivity.

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“…The haemolymph of bivalve molluscs, like mammalian blood, is an attractive tissue to use in assessing biomarker responses to environmental stressors, such as hypoxia (Monari et al 2005), parasite infestation (Muñoz et al 2006), radionuclides (Jha et al 2005), metals (Tran et al 2007) and organic pollutants (Galloway et al 2002;Pan et al 2006;Rickwood and Galloway 2004). Both haemocytes and cellfree haemolymph (plasma) have been used in such investigations, e.g.…”

Section: Introductionmentioning

confidence: 99%

Contamination of bivalve haemolymph samples by adductor muscle components: implications for biomarker studies

2008

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Haemolymph samples and haemocytes collected via the adductor muscles of bivalve molluscs are extensively used in ecotoxicological studies. Withdrawal of haemolymph from mussels, Mytilus edulis, via the posterior adductor muscle, may lead to contamination with the intracellular contents of adductor myocytes. Lysopine dehydrogenase (LyDH) activity, an adductor myocyte marker, was used to investigate the impact of this potential contamination on levels of total glutathione, glutathione peroxidase (GPx) and acetylcholinesterase (AChE) measured in cell-free haemolymph. The mean glutathione content of cell-free haemolymph from 28 mussels was 3.2 +/- 1.8 microM (mean +/- SD). There was a linear relationship (slope = 0.28 +/- 0.03 min; mean +/- SE; P < 0.0001, n = 28) with haemolymph LyDH levels suggesting that at least some of the glutathione measured in cell-free haemolymph had arisen from contamination. Haemolymph LyDH activity was significantly higher in samples extracted using larger diameter needles, and also in samples where there had been some difficulty in the extraction. Exposure of mussels to oxidative stress using 40 microg l(-1) Cu for 5 days resulted in a 1.7 fold increase in glutathione (P = 0.033), but no increase (P = 0.810) in LyDH activity in adductor muscle. This was reflected in a similar increase in the slope of a plot of cell-free haemolymph glutathione versus LyDH activity (P = 0.011), consistent with both of these having originated from the adductor muscle. Cell-free haemolymph GPx and AChE activities also correlated with LyDH activity (Spearman rank correlation coefficients of 0.531 (P = 0.0068) and 0.537 (P = 0.0062), respectively, n = 27) suggesting that these also arise from contamination of the haemolymph. For GPx there was a significant linear relationship (P = 0.025) with haemolymph LyDH levels consistent with both enzymes originating from the myocytes. However, there was hyperbolic relationship (P = 0.0004) between haemolymph AChE and LyDH activities. It appears that this is because the AChE originates from a different compartment to the LyDH, i.e. cholinergic neuromuscular junctions in the adductor muscle. We conclude that it would be prudent, when considering the possibility of using a biomarker in cell-free haemolymph from bivalve molluscs, to check whether contamination could be an issue.

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Protective effects of selenium on mercury-induced DNA damage in mussel haemocytes

Cited by 77 publications

References 34 publications

Civa Klorürün mtDNA'da Akut Etkisi

Civa Klorürün mtDNA'da Akut Etkisi

Use of Selenium to Detect Mercury in Water and Cells: An Enhancement of the Sensitivity and Specificity of a Seleno Fluorescent Probe

Contamination of bivalve haemolymph samples by adductor muscle components: implications for biomarker studies

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