Protective effects of selenium and nano-selenium on bisphenol-induced reproductive toxicity in male rats

Abstract

<jats:p> Bisphenol A (BPA) is a widespread compound associated with the manufacture of many consumer products. The BPA-induced reproductive toxicity was reported to be mainly attributed to oxidative stress. However, the role of antioxidants usage to decrease the injurious effects of BPA, on male reproductive functions, remains to unveil. The present research is established to evaluate the role of selenium (Se) and its nano form (NSe) as protective agents to alleviate BPA-induced testicular toxicity. Ninety mature albino male rats were assigned into six equal groups: negative control; orally BPA 150 mg/kg; Se 3 mg/kg; NSe 2 mg/kg; both BPA 150 mg/kg and Se 3 mg/kg; and BPA 150 mg/kg + NSe 2 mg/kg. The experiment lasted for 70 consecutive days, and then serum was collected for estimation of prostatic acid phosphatase. Testicular tissues were subjected to measurement of antioxidant status, lipid peroxidation, DNA damage, and expression of some apoptotic genes. Our results reported that BPA-induced marked testicular damage evidenced by significant elevations in serum prostatic acid phosphatase activity, malondialdehyde levels, a decrease in testicular catalase activity and reduced glutathione level. Moreover, marked DNA internucleosomal fragmentation pattern as well as upregulation of cyclooxygenase-2 and estrogen receptor-2 NSe genes were detected. Coadministration of Se and NSe attenuated the reproductive toxicity induced by BPA via improvement of the antioxidant activity, genetic changes, and restoration of testicular tissue nearly as control one. These results indicated that both Se and NSe forms could be used as reproductive protective agents against the detrimental effect induced by BPA. However, the NSe surpassed the selenium in modulating the DNA laddering, and the studied gene expression levels, and offered a potent reproductive protection. </jats:p>

Studies on the phytomodulatory potential of fenugreek ( Trigonella foenum‐graecum ) on bisphenol‐A induced testicular damage in mice

“…Similar results were detected by Khalaf et al (2019) where it was reported that BPA exposed group could promote apoptosis of germ cells. Data are analysed by one-way ANOVA followed by post hoc test (LSD), 1 p ≤ .001, 2 p ≤ .01, 3 p ≤ .05.…”

“…Similar results were detected by Khalaf et al (2019) where it was reported that BPA exposed group could promote apoptosis of germ cells.…”

“…Ultimately, these signals result in changes in gene expression, which influence the ability of the cell survival and apoptosis (Khalaf, Ahmed, Moselhy, Abdel-Halim, & Ibrahim, 2019). It has been also delineated that apoptosis of germ cells as well as generation of oxidative stress is a result of ROS generation by BPA which further compromises the capacity of leydig cells to be steroidogenic and ability to differentiate the germinal epithelium into normal spermatozoa (Hales et al, 2005;Skinner, Manikkam, & Guerrero-Bosagna, 2010).…”

Ameliorating effect of selenium nanoparticles on cyclophosphamide-induced hippocampal neurotoxicity in male rats: light, electron microscopic and immunohistochemical study

“…Nano Se played important roles in the enhancement of the antioxidant defense system and scavenging free radicals by increasing activities of both and glutathione Stransferase and Glutathione peroxidase [20,40,58] In the present study, the thickness of the neurocyte cell layers (pyramidal and granule layers) was significantly reduced in the group treated with CPH. This reduction occurred due to the decrease in the neurocyte population owing to the cellular damage (necrosis and/or apoptosis).…”

Antioxidant and Anti-Inflammatory Effects of Nano-Selenium against Cypermethrin-Induced Liver Toxicity

“…Se plays a significant role in antioxidant defense systems and it is important for fertility, growth, and immunity in human and animals [4]. Nano-selenium (NSe) showed high biological efficiency due to its good absorptive ability, high bioavailability, high surface activity and low toxicity comparing to other forms of Se [7]. NSe displays potent effects on upregulation of GPx and induction of glutathione S-transferase which resulted in less oxidative stress [8].…”