Ameliorating effect of selenium nanoparticles on cyclophosphamide-induced hippocampal neurotoxicity in male rats: light, electron microscopic and immunohistochemical study

Ibrahim, Hassan; Zommara, Mohsen; El‐Naggar, Mehrez E.

doi:10.5603/fm.a2020.0117

Cited by 23 publications

(17 citation statements)

References 42 publications

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“…The current study tried to elucidate the histologic changes of rat's hippocampus after postnatal exposure to acetaminophen via light microscope and it showed that hippocampal sections of rats which received 60 mg/kg/day of acetaminophen from PND 7 to PND 14 exhibited several changes in different parts of hippocampus using H and E stains including a reduction in the thickness of the pyramidal layer of Cornue Ammonis, presence of areas that are lacking of cells, presence of Ghost like cells as an indicative features of neural cell death of pyramidal cells in hippocampus, degenerated neurocytes in pyramidal layer among very few healthy cells beside clumping pyramidal cell nuclei. These findings are consistently shown in another work which suggested that these lesions were result from damage to neurons and lead to obvious reduction in the thickness of neurocytic layer (11,21,22). On the other hand, light microscopic evaluation of sections from DG region that are belonged to the rats which received 60 mg/kg /day of acetaminophen from PND 7 to PND 14 subcutaneously revealed different changes including presence of large neurons in the molecular layer, vacuolations in the granular layer of DG with features of disorganization in DG.…”

Section: Discussionsupporting

confidence: 82%

“…Dehydration (by serial dilution of ethyl alcohol), clearing (by xylol), and paraffin embedding of specimens were performed. Parasagittal sections of five microns (μm) were taken for all blocks to be ready for H&E staining (19)(20)(21) to be ready for blinded examination under Bright field Olympus light microscope(Japan)light microscope. The photomicrographs of structural changes were obtained by digital camera attached via plan apochromatic objectives.…”

Section: Study Ending and Histopathological Assessmentmentioning

confidence: 99%

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Histological changes of CA and DG regions of hippocampus of rats’ brain after exposure to Acetaminophen in postnatal period

Hussin¹,

Khalel²

2021

IJVS

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Authors reported that exposure to acetaminophen postnatally may be linked to increasing the risk of ASD. However, the reports on its effects on the brain are scanty, and the knowledge concerning their safety is little as there is a traditional left out of the clinical trials by users. The present work tried to elucidate the histological effects of acetaminophen on the hippocampus of neonate male rats after early postnatal exposure. The pups were categorized into2 groups, the control group, and the acetaminophen treated group. The acetaminophen treated animals were injected subcutaneously with acetaminophen of 60 mg/Kg/day from postnatal day (PND) 7 to PND 14, while the control group treated with normal saline with a similar approach. The histopathologic assessment revealed a diminishing in the pyramidal cells layer thickness of Cornue Ammonis. Some areas are devoid of cells with the appearance of Ghost like cells indicating features of neural cell death, degenerated neurons in the pyramidal layer are noticed. Features of nuclear clumping of pyramidal cell layer were shown. Moreover, several changes including vacuolations in the granular layer of DG with disorganization in DG. Neuronal processes presented with clumping. Apoptosis in the granular cells layer and hilus of a section of DG with the appearance of many astrocytes and microglial cells. Exposures to clinically relevant doses of acetaminophen in the postnatal period were shown to affect the histology of rat hippocampal regions, and a balanced risk assessment based on the best professional judgment must be prioritized.

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Section: Discussionsupporting

confidence: 82%

Section: Study Ending and Histopathological Assessmentmentioning

confidence: 99%

Histological changes of CA and DG regions of hippocampus of rats’ brain after exposure to Acetaminophen in postnatal period

Hussin¹,

Khalel²

2021

IJVS

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“…Although no previous reports have examined the effects of PDGs, either alone or combined with SeNPs, on GFAP expression in brain tissue, Se and SeNPs were found to dampen apoptotic cell death processes in the hippocampus, increase GFAP expression, reduce reactive astrogliosis, increase neuronal survival, and mitigate overall neuronal damage. 85 , 108 …”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Efficiency of Prodigiosins Conjugated with Selenium Nanoparticles in Rats Exposed to Chronic Unpredictable Mild Stress is Mediated Through Antioxidative, Anti-Inflammatory, Anti-Apoptotic, and Neuromodulatory Activities

Albrakati¹,

Alsharif²,

omairi³

et al. 2021

IJN

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Purpose Depression is a mood disorder accompanied by intensive molecular and neurochemical alterations. Currently, available antidepressant therapies are not fully effective and are often accompanied by several adverse impacts. Accordingly, the ultimate goal of this investigation was to clarify the possible antidepressant effects of prodigiosins (PDGs) loaded with selenium nanoparticles (PDGs-SeNPs) in chronic unpredictable mild stress (CUMS)-induced depression-like behavior in rats. Methods Sixty Sprague Dawley rats were randomly allocated into six groups: control, CUMS group (depression model), fluoxetine (Flu, 10 mg/kg)+CUMS, PDGs+CUMS (300 mg/kg), sodium selenite (Na 2 SeO 3 , 400 mg/kg)+CUMS, and PDGs-SeNPs+CUMS (200 mg/kg). All treatments were applied orally for 28 consecutive days. Results PDGs-SeNPs administration prevented oxidative insults in hippocampal tissue, as demonstrated by decreased oxidant levels (nitric oxide and malondialdehyde) and elevated innate antioxidants (glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase, and catalase), in addition to the upregulated expression of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 in rats exposed to CUMS. Additionally, PDGs-SeNPs administration suppressed neuroinflammation in hippocampal tissue, as determined by the decreased production of pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1β, and interleukin-6), increased anti-inflammatory cytokine interleukin-10, and decreased inflammatory mediators (prostaglandin E2, cyclooxygenase-2, and nuclear factor kappa B). Moreover, PDGs-SeNPs administration in stressed rats inhibited neuronal loss and the development of hippocampal apoptosis through enhanced levels of B cell lymphoma 2 and decreased levels of caspase 3 and Bcl-2-associated X protein. Interestingly, PDGs-SeNPs administration improved hormonal levels typically disrupted by CUMS exposure and significantly modulated hippocampal levels of monoamines, brain-derived neurotrophic factor, monoamine oxidase, and acetylcholinesterase activities, in addition to upregulating the immunoreactivity of glial fibrillary acidic protein in CUMS model rats. Conclusion PDGs-SeNPs may serve as a prospective antidepressant candidate due to their potent antioxidant, anti-inflammatory, and neuroprotective potential.

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“…SeNPs have been reported to protect nerve cells from neurological disorders [ 32 ]. In this study, the influence of SeNPs on the proliferation and differentiation of NSCs was further explored.…”

Section: Resultsmentioning

confidence: 99%

Selenium nanoparticles derived from Proteus mirabilis YC801 alleviate oxidative stress and inflammatory response to promote nerve repair in rats with spinal cord injury

Liu

Mao

Huang

et al. 2022

Regenerative Biomaterials

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Microbial biotransformation and detoxification of biotoxic selenite into selenium nanoparticles (SeNPs) has emerged as an efficient technique for the utilization of selenium. SeNPs are characterized by high bioavailability and have several therapeutic effects owing to their antioxidant, anti-inflammatory, and neuroprotective activities. However, their influence on microenvironment disturbances and neuroprotection after spinal cord injury (SCI) is yet to be elucidated. This study aimed to assess the influence of SeNPs on SCI and explore the underlying protective mechanisms. Overall, the proliferation and differentiation of neural stem cells (NSCs) were facilitated by SeNPs derived from Proteus mirabilis YC801 via the Wnt/β-catenin signaling pathway. The SeNPs increased the number of neurons to a greater extent than astrocytes after differentiation and improved nerve regeneration. A therapeutic dose of SeNPs remarkably protected the integrity of the spinal cord to improve the motor function of the hind limbs after SCI, and decreased the expression of several inflammatory factors such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in vivo and enhanced the production of M2-type macrophages by regulating their polarization, indicating the suppressed inflammatory response. Besides, SeNPs reversed the SCI-mediated production of reactive oxygen species. In conclusion, SeNPs treatment holds the potential to improve the disturbed microenvironment and promote nerve regeneration, representing a promising therapeutic approach for SCI.

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Ameliorating effect of selenium nanoparticles on cyclophosphamide-induced hippocampal neurotoxicity in male rats: light, electron microscopic and immunohistochemical study

Abstract: This article has been peer reviewed and published immediately upon acceptance. It is an open access article, which means that it can be downloaded, printed, and distributed freely, provided the work is properly cited. Articles in "Folia Morphologica" are listed in PubMed.

Cited by 23 publications

References 42 publications

Histological changes of CA and DG regions of hippocampus of rats’ brain after exposure to Acetaminophen in postnatal period

Histological changes of CA and DG regions of hippocampus of rats’ brain after exposure to Acetaminophen in postnatal period

Neuroprotective Efficiency of Prodigiosins Conjugated with Selenium Nanoparticles in Rats Exposed to Chronic Unpredictable Mild Stress is Mediated Through Antioxidative, Anti-Inflammatory, Anti-Apoptotic, and Neuromodulatory Activities

Selenium nanoparticles derived from Proteus mirabilis YC801 alleviate oxidative stress and inflammatory response to promote nerve repair in rats with spinal cord injury

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