Protective effects of saffron (<i>Crocus sativus</i> Linn.) on genotoxins‐induced oxidative stress in Swiss albino mice

Premkumar, Kumpati; Abraham, Suresh K.; Santhiya, S.; Ramesh, A.

doi:10.1002/ptr.1209

Cited by 113 publications

(75 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The modifying effects of saffron and its carotenoids on antioxidant enzymatic activities have also been shown in other experimental models (Premkumar et al, 2001;Premkumar et al, 2003;Shen and Qian, 2006). Moreover, crocin and other carotenoids from saffron prevent the death of rat pheochromocytoma (PC-12) cells, apoptotic morphological changes, and DNA fragmentation induced by tumor necrosis factor (TNF)-a and serum=glucose deprivation by its antioxidant effects and increasing GSH synthesis (Soeda et al, 2001;Ochiai et al, 2004aOchiai et al, , 2004bOchiai et al, , 2007.…”

Section: Effect Of Saffron and Crocin On Mms-induced Dna Damagementioning

confidence: 67%

“…Radical scavenging, antioxidant activity, and promotion of the diffusivity of oxygen in different tissues were also reported for saffron extract or its bioactive constituents (Rios et al, 1996;Verma and Bordia, 1998;Assimopoulou et al, 2005;Kanakis et al, 2007a). Saffron extract also has chemopreventive and genoprotective effects and protects from genotoxin-induced oxidative stress in mice (Nair et al, 1995;Premkumar et al, 2001;Abdullaev et al, 2002;Premkumar et al, 2003).…”

Section: Introductionmentioning

confidence: 89%

“…It has been reported that saffron or its ingredients such as crocin and dimethylcrocin are not mutagenic or genotoxic (Salomi et al, 1991a;Abdullaev et al, 2002Abdullaev et al, , 2003. Premkumar et al also showed that saffron aqueous extract protects from genotoxicity as well as genotoxins-induced oxidative stress in mice (Premkumar et al, 2001(Premkumar et al, , 2003(Premkumar et al, , 2006. In these studies, oral pretreatment with aqueous saffron extract (20, 40, and 80 mg=Kg) for 5 consecutive days significantly inhibited the genotoxicity of antitumor drugs (cyclophosphamide, mitomycin C, and cisplatin), in vivo, as revealed by micronucleus and comet assay.…”

Section: Effect Of Saffron and Crocin On Mms-induced Dna Damagementioning

confidence: 99%

See 2 more Smart Citations

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Hosseinzadeh

Abootorabi

Sadeghnia

2008

DNA and Cell Biology

View full text Add to dashboard Cite

This study was designed to examine the effect of aqueous extract of Crocus sativus stigmas (CSE) and crocin (trans-crocin 4) on methyl methanesulfonate (MMS)-induced DNA damage in multiple mice organs using comet assay. Adult male NMRI mice in different groups were treated with either physiological saline (10 mL=Kg, intraperitoneal [ip]), CSE (80 mg=Kg, ip), crocin (400 mg=Kg, ip), MMS (120 mg=Kg, ip), and CSE (5, 20, and 80 mg=Kg, ip) 45 min prior to MMS administration or crocin (50, 200, and 400 mg=Kg, ip) 45 min prior to MMS administration. Mice were scarified about 3 h after each different treatment, and the alkaline comet assay was used to evaluate the effect of these compounds on DNA damage in different mice organs. The percent of DNA in the comet tail (% tail DNA) was measured. A significant increase in the % tail DNA was seen in nuclei of different organs of MMS-treated mice. In control groups, no significant difference was found in the % tail DNA between CSE-or crocin-pretreated and saline-pretreated mice. The MMS-induced DNA damage in CSEpretreated mice (80 mg=Kg) was decreased between 2.67-fold (kidney) and 4.48-fold (lung) compared to those of MMS-treated animals alone ( p < 0.001). This suppression of DNA damage by CSE was found to be depended on the dose, which pretreatment with CSE (5 mg=Kg) only reduced DNA damage by 6.97%, 6.57%, 7.27%, and 9.90% in liver, lung, kidney, and spleen, respectively ( p > 0.05 as compared with MMS-treated group). In the same way, crocin also significantly decreased DNA damage by MMS (between 4.69-fold for liver and 6.55-fold for spleen, 400 mg=Kg), in a dose-dependent manner. These data indicate that there is a genoprotective property in CSE and crocin, as revealed by the comet assay, in vivo.

show abstract

Section: Effect Of Saffron and Crocin On Mms-induced Dna Damagementioning

confidence: 67%

Section: Introductionmentioning

confidence: 89%

Section: Effect Of Saffron and Crocin On Mms-induced Dna Damagementioning

confidence: 99%

See 1 more Smart Citation

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Hosseinzadeh

Abootorabi

Sadeghnia

2008

DNA and Cell Biology

View full text Add to dashboard Cite

show abstract

“…According the free radicals theory, imbalance among pro oxidants and anti-oxidants eventually causes oxidative damages on cellular processes and steroidogenesis in lydic cells (Luo et al, 2006). Based on Premkumar et al (2003) report, saffron due to its carotenoid compounds results in regulating the peroxidase activity of lipids, antioxidants and detoxification. Ochiai et al (2004) have reported that saffron's crocines through indirect increasing of m-RNA enzyme, gamma glotamilsyteienilsyntethaseincrease the restored glutathione amount inside the cell and have a considerable importance in regulating the peroxidase activity of lipids and anti-oxidants.…”

Section: Resultsmentioning

confidence: 99%

Effect of Saffron on Histomorphometric Changes of Testicular Tissue in Rat

2011

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

Problem statement: Saffron is widely used as a food flavor and has well known medicinal effects. This drug has an effect on steroid and sexual hormones. The aim of this study was investigation of Saffron orally administration on histomorphometric change of testicular tissue in rat. In this experimental study, 40 male rats are used (4 groups and 10 rats in each group). Approach: Animals were received Saffron (50, 100 and 200 mg kg −1 ) daily orally administration, during 28 days. As one group was control (normal saline) and three groups was drug consumer. The testis tissue was sampled after passing of above time and section providing, was stained by H and E. Results: The histomorphometric changes of testicular tissue which include, seminiferous tubules diameter, seminiferous epithelial thickness, interstitial tissue and testicular capsule thickness were studied. Results showed that seminiferous tubules diameter, seminiferous epithelial thickness had significantly decreased (p<0.05) but interstitial tissue thickness had significantly increased (p<0.05) compared with control group. Testicular capsule thickness had not significantly changed. Conclusion: Our results showed that seminiferous tubules diameter, seminiferous epithelial thickness and interstitial tissue thickness in rat testis were significantly changed after Saffron orally administration. This results probably via the reducing of serum testosterone concentration. Effect of Saffron on spermatogenesis index and unfertilization in human needed to be more investigation.

show abstract

“…The previous studies have indicated that saffron elevates SOD, CAT and GSH-Px activities and inhibits the lipid peroxidation (Premkumar et al 2003). Recently, it has been reported that safranal, an active content of saffron, treats the cerebral ischemia-reperfusion induced oxidative stress and decreases the increased MDA levels (Hosseinzadeh et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Neuro-protective effects of Crocin on brain and cerebellum tissues in diabetic rats

Altinzo¹,

Oner²,

Elbe³

et al. 2015

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

Background: Increase in free oxygen radicals and the disruption of defense system make the neurons and astrocytes more sensitive against oxidative damage. Materials and Methods: Rats were divided into three groups containing 10, rats in each group namely: control (C) group, Diabetes Mellitus (DM) group, and Diabetes Mellitus + crocin (DM+crocin) group. Tissue samples were processed by routine histological and biochemical procedures. The sections were stained with Hematoxylen-eosin. Malondialdehyde (MDA), glutathione (GSH), blood glucose, HbA1c levels and xanthine oxidase (XO) activities were assayed. Results: The histological appearence of the cerebrum and cerebellum were normal in the control group. DM group showed some histopathological changes including congestion, perivascular and perineuronal edema in cerebrum. In DM + crocin group, histopathological changes in cerebrum and cerebellum markedly reduced. MDA level and XO activities increased significantly in DM group (P<0.01), but decreased significantly in DM + crocin group when compared to DM group (P<0.01). Blood glucose concentrations increased significantly (p<0.01) in DM group), but decreased significantly in DM + crocin group when compared with DM group (p<0.05). Blood HbA1c levels were normal in control group. But there were significant differences between control and DM groups (p<0.01). On the other hand, blood HbA1c levels decreased in DM+crocin group when compared with the DM group, but it was not statistically significant (p > 0.05). Conclusion: Due to the fact that crocin has an antioxidant and anti-hyperglycemic effects, it can protect the brain and cerebellum tissue against the complications of oxidative stress.

show abstract

Protective effects of saffron (Crocus sativus Linn.) on genotoxins‐induced oxidative stress in Swiss albino mice

Cited by 113 publications

References 27 publications

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Effect of Saffron on Histomorphometric Changes of Testicular Tissue in Rat

Neuro-protective effects of Crocin on brain and cerebellum tissues in diabetic rats

Contact Info

Product

Resources

About